SpringerLink
Log in

A comparative study on effect of heat stress on physiological and cellular responses of crossbred cattle and riverine buffalo in subtropical region of India

  • Original Paper
  • Published:
International Journal of Biometeorology Aims and scope Submit manuscript

Abstract

The study was carried out to compare the in vitro and in vivo heat shock responses of cattle and buffaloes. The expression of heat responsive genes (HSP70 and HSF family) were studied in vitro in peripheral blood mononuclear cells (PBMCs) of cattle and buffalo. In vivo observations on animals were carried out to investigate the physiological responses of cattle and buffalo at different THI over a period of 14 months. The study indicated that onset and severity of heat stress at different THI varied significantly between cattle and buffalo. Rectal temperature (RT) showed a significant (p < 0.05) increase at THI 67 in buffaloes and at THI 68 in cattle. Significant (p < 0.01) differences in RT between the species were observed at THI 71, 72, and 73. Respiration rate (RR) significantly (p < 0.05) increased at THI 70 in both the species and significant (p < 0.05) differences in RR were observed between the species at THI 65, 68, 69, and 74. THI had significant (p < 0.05) effect on blood glucose and blood electrolytes of the species with increased levels at higher THI. Serum AST and ALT levels showed less pronounced changes over increasing THI. Heat stress-associated expressions of HSP 70 genes followed temporal changes with incremental THI. The expression of HSPA8 was consistent at lower THI whereas upregulation of HSPA1A and HSPA1L was evident at higher THI. The study concludes that changes in physiological parameters such as RT and RR occur in a phasic pattern in both species and onset of heat stress was early in buffalo as compared to cattle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Aggarwal A, Singh M (2008) Changes in skin and rectal temperature in lactating buffaloes provided with showers and wallowing during hot-dry season. Trop Anim Health Prod 40(3):223–228. https://doi.org/10.1007/s11250-007-9084-3

    Article  Google Scholar 

  • Akerfelt M, Morimoto RI, Sistonen L (2010) Heat shock factors: integrators of cell stress, development and lifespan. Nat Rev Mol Cell Biol 11(8):545–555

    Article  CAS  Google Scholar 

  • Ammer S, Lambertz C, von Soosten D, Zimmer K, Meyer U, Dänicke S, Gauly M (2018) Impact of diet composition and temperature-humidity index on water and dry matter intake of high-yielding dairy cows. J Anim Physiol Anim Nutr (Berl) 102(1):103–113

    Article  CAS  Google Scholar 

  • Banerjee D, Ashutosh (2011) Effect of thermal exposure on diurnal rhythms of physiological parameters and feed, water intake in Tharparkar and Karan Fries heifers. Biol Rhythm Res 42(1):39–51

  • Bernabucci U, Lacetera N, Baumgard LH, Rhoads RP, Ronchi B, Nardone A (2010) Metabolic and hormonal acclimation to heat stress in domesticated ruminants. Animal 4(7):1167–1183

    Article  CAS  Google Scholar 

  • Bertoni A, Fabio N, Daniel MR, Emilio S, Adolfo ÁM, Patricia MM, Armando MC, Jesús BG, Isabel GL (2020) Similarities and differences between river buffaloes and cattle: health, physiological, behavioral and productivity aspects. J Buffalo Sci 9:92–109

    Article  CAS  Google Scholar 

  • Bhan C, Singh SV, Hooda OK, Upadhyay RC, Beenam MV (2012) Influence of temperature variability on physiological, hematological and biochemical profile of growing and adult sahiwal cattle. J Environ Res Dev 7(2):986–994

    Google Scholar 

  • Chaiyabutr N, Buranakarl C, Loypetjra P, Chanpongsang S (1990) Effects of prolonged exposure to solar radiation on body water turnover and volume of the blood of non-shaded swamp buffaloes. Am J Anim Sci 3(1):47–52

    Google Scholar 

  • Chaiyabutr N, Burassakurl C, Moanbchurogen V, Loypetjra P, Pichaichrnatong A (1987) Effect of acute heat stress on changes in the rate of liquid flow from the rumen and turnover of swamp buffalo. J Agric 108:549–553

    Google Scholar 

  • Cincovic MR, Belic B, Toholj B, Potkonjak A, Stevancevic M, Lako B, Radovic I (2013) Metabolic acclimation to heat stress in farm housed Holstein cows with different body condition scores. Afr J Biotechnol 10(50):10293–10303

    Google Scholar 

  • Coles EH (1986) Veterinary Clinical Pathology, 4th Ed. W.B. Saunders Company, London

  • Collier RJ, Collier JL, Rhoads RP, Baumgard LH (2008) Invited review: genes involved in the bovine heat stress response. J Dairy Sci 91(2):445–454

    Article  CAS  Google Scholar 

  • Deka K, Saha S (2018) Regulation of mammalian HSP70 expression and stress response. In: Asea A, Kaur P (eds) Regulation of heat shock protein responses. Heat Shock Proteins, vol 13. Springer, Cham. https://doi.org/10.1007/978-3-319-74715-6_1

  • Desta TT (2012) Introduction of domestic buffalo (Bubalus bubalis) into Ethiopia would be feasible. Renew Agric Food Syst 27(4):305–313

    Article  Google Scholar 

  • Ganaie AH, Shanker G, Bumla NA, Ghasura RS, Mir NA (2013) Biochemical and physiological changes during thermal stress in bovines. J Vet Sci Technol 4:126

    Google Scholar 

  • Gaughan JB, Mader TL, Holt SM, Sullivan ML, Hahn GL (2010) Assessing the heat tolerance of 17 beef cattle genotypes. Int J Biometeorol 54:617–627

  • Hahn GL (1999) Dynamic responses of cattle to thermal heat loads. J Anim Sci 77(suppl. 2):10–20

    CAS  Google Scholar 

  • Haque N, Ludri A, Hossain SA, Ashutosh M (2013) Impact on haematological parameters in young and adult Murrah buffaloes exposed to acute heat stress. Buffalo Bull 32(2):321–326

    Google Scholar 

  • Hooda OK, Singh G (2009) Changes in serum electrolytes, minerals and physiological responses in buffalo heifers exposed to high temperature in a psychrometric chamber. Indian J Anim Nutri 26:327–332

    CAS  Google Scholar 

  • Horowitz M (2001) Heat acclimation: phenotypic plasticity and cues to the underlying molecular mechanisms. J Thermal Biol 26(4–5):357–363

    Article  CAS  Google Scholar 

  • Horowitz M (2002) From molecular and cellular to integrative heat defense during exposure to chronic heat. Comp Biochem Physiol Part A: Mol Integr Physiol 131(3):475–483

    Article  Google Scholar 

  • Horowitz M, Kaspler P, Marmary Y, Oron Y (1996) Evidence for contribution of effector organ cellular responses to the biphasic dynamics of heat acclimation. J Appl Physiol 80(1):77–85

    Article  CAS  Google Scholar 

  • Hu H, Zhang Y, Zheng N, Cheng J, Wang J (2016) The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells. Anim Sci J 87(1):84–91

    Article  CAS  Google Scholar 

  • Jeelani R, Konwar D, Khan A, Kumar D, Chakraborty D, Brahma B (2019) Reassessment of temperature-humidity index for measuring heat stress in crossbred dairy cattle of a sub-tropical region. J Thermal Biol 82:99–106

    Article  Google Scholar 

  • Joo SS, Lee SJ, Park DS, Kim DH, Gu BH, Park YJ, Rim CY, Kim M, Kim ET (2021) Changes in blood metabolites and immune cells in Holstein and Jersey dairy cows by heat stress. Animals 11(4):974

  • Joshi BC, McDowell RE, Sadhu DP (1968) Body surface evaporation rates at low and high temperatures in Murrah buffalo. J Dairy Sci 51(10):1689–1692

    Article  CAS  Google Scholar 

  • Kam**a HH, Hageman J, Vos MJ, Kubota H, Tanguay, et al (2009) Guidelines for the nomenclature of the human heat shock proteins. Cell Stress Chaperones 14:105–111

    Article  CAS  Google Scholar 

  • Kaneco JJ, Harvey JW, Bruss ML (2008) Clinical biochemistry of domestic animals (6 Ed). Academic Press Elsevier, London, 351–379

  • Kapila N, Sharma A, Kishore A, Sodhi M, Tripathi PK, Mohanty AK, Mukesh M (2016) Impact of heat stress on cellular and transcriptional adaptation of mammary epithelial cells in riverine buffalo (Bubalus bubalis). PLoS One 11(9):e0157237

    Article  Google Scholar 

  • Kishore A, Sodhi M, Kumari P, Mohanty AK, Sadana DK, Kapila N, Khate K, Shandilya U, Kataria RS, Mukesh M (2014) Peripheral blood mononuclear cells: a potential cellular system to understand differential heat shock response across native cattle (Bos indicus), exotic cattle (Bos taurus), and riverine buffaloes (Bubalus bubalis) of India. Cell Stress Chaperones 19:613–621

    Article  CAS  Google Scholar 

  • Koga A, Kurata K, Furukawa R, NAKAJiMA M, Hommma H, Kanai Y, Chikamune T (1998) Rectal-skin temperature difference regulated by blood volume in swamp buffaloes in hot conditions: comparative study of thermo-regulation in buffaloes and cattle. AnimSci Technol 69:81-89

  • Korde JP, Singh G, Varshney VP, Shukla DC (2007) Effects of long-term heat exposure on adaptive mechanism of blood acid-base in buffalo calves. Asian-Australas J Anim Sci 13:329–332

    Google Scholar 

  • Krishnawoorthy M, Prabhu A (2011) Anticancer activity of cynodondoctylen L.Extact of enrich ascites carcinoma. J Environ Res Dev 5(3):551–557

  • Kumar SBV, Singh G, Meur SK (2010) Effects of addition of electrolyte and ascorbic acid in feed during heat stress in buffaloes. Asian-Australas J Anim Sci 23:880–888

    Article  Google Scholar 

  • Landaeta-Hernández A, Zambrano-Nava S, Hernández-Fonseca JP, Godoy R, Calles M, Iragorri JL, Añez L, Polanco M, Montero-Urdaneta M, Olson T (2011) Variability of hair coat and skin traits as related to adaptation in Criollo Limonero cattle. Trop Anim Health Prod 43(3):657–663

    Article  Google Scholar 

  • Lemerle C, Goddard ME (1986) Assessment of heat stress in dairy cattle in Papua New Guinea. Trop Anim Health Prod 18(4):232–242

    Article  CAS  Google Scholar 

  • Li GC, Mak JY (1989) Re-induction of hsp70 synthesis: an assay for thermotolerance. Int J Hyperthermia 5(3):389–403

    Article  CAS  Google Scholar 

  • Maloyan A, Horowitz M (2002) β-Adrenergic signaling and thyroid hormones affect HSP72 expression during heat acclimation. J Appl Physiol 93(1):107–115

    Article  CAS  Google Scholar 

  • Marai IFM, Habeeb AAM, Daader AH, Yousef HM (1997) Effects of diet supplementation and body cooling on Friesian calves reared in high ambient temperature in the Eastern Desert of Egypt. Trop Anim Health Prod 4:201–208

    Article  Google Scholar 

  • NRC (1971) A guide to environmental research on animals, vol 12. National Academy of Science, Washington, DC, pp 57–59

    Google Scholar 

  • Pandey P, Hooda OK, Kumar S (2017) Impact of heat stress and hypercapnia on physiological, hematological, and behavioral profile of Tharparkar and Karan Fries heifers. Vet World 10(9):1146

    Article  Google Scholar 

  • Patel JS, Jajane KR, Vadqaria VP, Kullarni VV, Radadia NS (1991) Effect of physiological traits as affected by heat stress (a review). Livestock Prod Sci 78:71–90

    Google Scholar 

  • Patir H, Upadhyay RC (2010) Purification, characterization and expression kinetics of heat shock protein 70 from Bubalus bubalis. Res Vet Sci 88(2):258–262

    Article  CAS  Google Scholar 

  • Paudel TP, Acharya BR, Karki DB, Shrestha BS (2018) Effect of heat stress on crossbred dairy cattle in tropical Nepal: impact on blood parameters. J Agric Nat Resour 1(1):223–230

    Article  Google Scholar 

  • Riley DG, Chase CC Jr, Coleman SW, Olson TA (2012) Genetic assessment of rectal temperature and coat score in Brahman, Angus, and Romosinuano crossbred and straight bred cows and calves under subtropical summer conditions. Livestock Sci 148(1–2):109–118

    Article  Google Scholar 

  • Ronchi B, Bernabucci U, Lacetera NG, Supplizi AV, Nardone A (1999) Distinct and common effects of heat stress and restricted feeding on metabolic status of Holstein heifers. Zootec Nutr Anim 25:11–20

    Google Scholar 

  • Schmittgen T, Livak K (2008) Analyzing real-time PCR data by the comparative CT method. Nat Prot 3:1101–1108

    Article  CAS  Google Scholar 

  • Singh M, Roy PK, Sharma DK, Pal PK (2010) Effect of Non-genetic factors on milk production performance of cows in eastern region. Indian J Dairy Sci 62(4):313–315

    Google Scholar 

  • Swenson MJ (1984) Physiological properties, cellular and chemical constituents of blood. In: Swenson MJ (ed) Dukes’ physiology of domestic animals, 10th edn. Cornstock Publishing Associates, Cornell University Press, Ithaca, London, pp 15–40

    Google Scholar 

  • Umar SIU, Konwar D, Khan A, Bhat MA, Javid F, Jeelani R, Brahma B (2021) Delineation of temperature-humidity index (THI) as indicator of heat stress in riverine buffaloes (Bubalus bubalis) of a sub-tropical Indian region. Cell Stress Chaperones 26(4):657–669

    Article  CAS  Google Scholar 

  • Vaidya M, Kumar P, Singh VS (2010) Circadian variations in heat production and heat loss in Murrah buffaloes during different season. Revista Veterinaria 21(1)

  • Wang Shiying, Edens FW (1998) Heat conditioning induces heat shock proteins in broiler chickens and turkey poults. Poultry Sci 77(11):1636–1645

    Article  CAS  Google Scholar 

  • Zicarelli L (2016) “Estacionalidad Reproductiva en Búfalas”, en: Crudeli, G. et al. (comp.), Reproducción en Búfalas, Ediciones Moglia, Argentina

  • Zulkifli I, Norbaiyah B, Cheah YW, Soleimani AF, Sazili AQ, Goh YM (2010) A note on heat shock protein 70 expression in goats subjected to road transportation under hot, humid tropical conditions. Animal 4:973–976

    Article  CAS  Google Scholar 

Download references

Funding

The authors express their sincere thanks and acknowledge the financial support by Science and Engineering Research Board, Department of Science and Technology, Government of India (Grant no. EMR/2016/002845/AS and CRG/2019/001107), for funding this study.

Author information

Authors and Affiliations

  1. Division of Livestock Production Management, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, R. S. Pura, Jammu & Kashmir, 181102, India

    Sutikshan Sharma, Vaishali Sharma, Dipanjali Konwar, Asma Khan & Biswajit Brahma

  2. Division of Animal Genetics and Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, Jammu, India

    Dhirendra Kumar

Authors
  1. Sutikshan Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vaishali Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dipanjali Konwar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Asma Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dhirendra Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Biswajit Brahma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Biswajit Brahma.

Ethics declarations

The authors declare no financial or non-financial competing interest in publication of this research work. The funding agency has no role in publication of this research. All the authors have read and agreed to submit this work to the journal for publication.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 19 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sharma, S., Sharma, V., Konwar, D. et al. A comparative study on effect of heat stress on physiological and cellular responses of crossbred cattle and riverine buffalo in subtropical region of India. Int J Biometeorol 67, 1619–1628 (2023). https://doi.org/10.1007/s00484-023-02523-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00484-023-02523-2

Keywords

Search

Navigation