Abstract
Southern Corn Leaf Blight (SCLB) is an important disease in warm-temperate and tropical corn-producing areas throughout the world. We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked to the rhm gene for resistance to SCLB. One co-dominant AFLP marker, p7m36, was mapped to a position 1.0 cM from rhm, and we converted this marker to an STS (sequence-tagged site) marker. Combined with the previously identified agrP144, this new marker may be useful for map-based cloning of the rhm gene and marker-assisted selection for rhm.
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Acknowledgements
We thank Dr. H. Morishima (Tokyo University of Agriculture, Tokyo, Japan) and the two anonymous reviewers for their valuable comments on this manuscript. We also acknowledge Dr. A. A. Myburg (North Carolina State University, Raleigh, NC, USA) for his technical advice on AFLP analysis. This study was supported by a grant from the Japan Racing Association
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Communicated by R. Hagemann
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Cai, Hw., Gao, Zs., Yuyama, N. et al. Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis. Mol Gen Genomics 269, 299–303 (2003). https://doi.org/10.1007/s00438-003-0837-z
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DOI: https://doi.org/10.1007/s00438-003-0837-z