Abstract
Cryptosporidium spp. are protozoa commonly found in domestic and wild animals. Limited information is available on Cryptosporidium in deer worldwide. In this study, 201 fecal samples were collected from Alpine musk deer on three farms in Gansu Province, China. Detection and subty** of Cryptosporidium were performed by PCR and sequence analysis of the SSU rRNA and gp60 genes. The prevalence of Cryptosporidium infection in Alpine musk deer was 3.9% (8/201), with infection rates of 1.0% (1/100), 2.8% (1/36), and 9.2% (6/65) in three different farms. All positive samples for Cryptosporidium were from adult deer. Two Cryptosporidium species were identified, including C. parvum (n = 2) and C. xiaoi (n = 6). The C. parvum isolates were subtyped as IIdA15G1, while the C. xiaoi isolates were subtyped as XXIIIa (n = 2) and XXIIIg (n = 4). The IIdA15G1 subtype of C. parvum was found for the first time in deer. These results provide important insights into the identity and human infectious potential of Cryptosporidium in farmed Alpine musk deer.
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Data availability
The gp60 gene sequences generated in this study have been deposited in GenBank under accession numbers PP114495–PP14502.
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Funding
This work was supported by the Program for Young and Middle-aged Leading Science, Technology, and Innovation of the **njiang Production and Construction Group (2018CB034).
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Z. Z.hang and M. Q. conceived and designed the study. M. Q. collected the fecal samples. P. L., B. Z., Y. Z., R. C. and F. Y. carried out the PCR assays and sequence analyses. Z. Z. and P. L. interpreted the results and drafted the manuscript. All authors have reviewed and approved the final version of the manuscript.
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Li, P., Zhang, B., Zhao, Y. et al. Molecular detection of Cryptosporidium in Alpine musk deer (Moschus chrysogaster) in Gansu Province, Northwest China. Parasitol Res 123, 231 (2024). https://doi.org/10.1007/s00436-024-08252-8
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DOI: https://doi.org/10.1007/s00436-024-08252-8