Abstract
Lymnaeids are aquatic snails playing an important role in the transmission of many parasitic trematode species of veterinary and medical importance. In this study, we assessed the presence of cercarial flukes in naturally infected lymnaeid snails from Phayao province, Thailand, and determined the species diversity of both the intermediate snail hosts and parasite larvae. A total of 3,185 lymnaeid snails were collected from paddy fields at 31 sites in eight districts of Phayao province between October 2021 and December 2022. Larval fluke infection was assessed using the cercarial shedding method. The collected snails as well as emerging cercariae were identified at the species level via morphological and molecular methods. The sequences of snail internal transcribed spacer region 2 (ITS2) and cercarial 28S ribosomal RNA gene (28S rDNA) and cytochrome C oxidase1 (Cox1) were determined by PCR amplification and sequencing. Three species of lymnaeid snails were detected in this study, including Radix (Lymnaea) rubiginosa (Michelin, 1831), Radix (Lymnaea) swinhoei (Adams, 1866) and Austropeplea viridis (Quoy & Gaimard, 1832), of which R. rubiginosa was the most abundant, followed by A. viridis and R. swinhoei. The overall rate of trematode cercarial infection in the lymnaeid snails was 2.8% (90/3,185); the cercarial infection rate in R. rubiginosa and A. viridis was 3.5% (60/1,735) and 3.1% (30/981), respectively. No larval fluke infection was observed in the studied R. swinhoei (0/469). Nine morphotypes of cercariae were detected at 15 sites from four districts. The emerging cercariae were molecularly identified as Clinostomum sp., Aporocotylidae sp., Apharyngostrigea sp., Trichobilharzia sp., Apatemon sp., Pegosomum sp., Petasiger sp., Echinostoma revolutum and Plagiorchis sp. These findings emphasize the occurrence and diversity of trematode cercariae among naturally infected lymnaeid snails in Phayao province and could contribute to broadening our understanding of the host-parasite relationships between trematodes and their first intermediate hosts as well as develo** effective interventions to control trematode parasitic diseases.
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Data availability
The sequences generated for the snail ITS2 and trematode 28S rDNA and Cox1 mtDNA in this study have been deposited in GenBank database under the following accession numbers: OR030136-159, OR030291-310 and OR030096-109.
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Acknowledgements
The authors are grateful for the technical assistance provided by Ms Thanawadee Chaiphutorn and Ms Wannapha Kanameung.
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This research project was supported by the Thailand Science Research and Innovation Fund and the University of Phayao (Grant No. FF65-RIM118), Phayao, Thailand.
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OJ conceived and designed the study. OJ, CS and CP surveyed and collected snail samples. OJ, CS, CP and PS performed parasite detection and identification. OJ, CP and TB carried out parasite drawing and imaging. OJ performed molecular work, sequencing and bioinformatics analyses. OJ, CS and CP collected, analysed and interpreted data. OJ wrote the first draft of the manuscript. OJ and CS revised and edited the manuscript. All authors read and approved the final manuscript.
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Suwancharoen, C., Phuangsri, C., Siriwechviriya, P. et al. Diversity of trematode cercariae among naturally infected lymnaeid snails from Phayao, Thailand. Parasitol Res 122, 2691–2708 (2023). https://doi.org/10.1007/s00436-023-07971-8
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DOI: https://doi.org/10.1007/s00436-023-07971-8