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Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation

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Abstract

A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs–Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the first such measurement made for a microbeam irradiation, and a six fold increment of oxygen flux induced during a 15-s period of time has been observed following radiation exposure. The experimental procedure and the results are discussed.

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Acknowledgments

We thank Dr. Alan Bigelow and Professor Charles Geard for their helpful discussions of the experiment and the results. This work was supported by the National Institute of Biomedical Imaging and Bioengineering (NIBIB grant P41EB002033) and by funding from the National Cancer Institute (NCI P01-CA49062-22).

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Authors and Affiliations

  1. Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA

    Yan** Xu, Gerhard Randers-Pehrson & David J. Brenner

  2. Center for Radiological Research, Columbia University, New York, NY, 10032, USA

    Bo Zhang & Tom K. Hei

  3. Biocurrents Research Center, Marine Biological Laboratory, Woods Hole, MA, 02543, USA

    Mark Messerli

  4. Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA

    Mark Messerli

Authors
  1. Yan** Xu
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  2. Bo Zhang
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  3. Mark Messerli
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  4. Gerhard Randers-Pehrson
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  5. Tom K. Hei
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  6. David J. Brenner
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Corresponding author

Correspondence to Yan** Xu.

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Xu, Y., Zhang, B., Messerli, M. et al. Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation. Radiat Environ Biophys 54, 137–144 (2015). https://doi.org/10.1007/s00411-014-0574-1

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  • DOI: https://doi.org/10.1007/s00411-014-0574-1

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