Abstract
Purpose
The changes of leukotriene B4 (LTB4) in the serum and the 5-lipoxygenase (5-LO) in the decidua in different stages of gestation period were investigated.
Methods
The ELISA double antibody sandwich method was used to detect the concentration of LTB4 in the serum in 21 cases and its levels of the different stages of pregnancy were analyzed. The real-time PCR method was used to detect the mRNA expression level of 5-LO in the decidua tissue of 20 healthy women in early pregnancy and 21 healthy women in third trimester and its levels in the two stages of pregnancy were compared.
Results
There were significantly different in the concentration of LTB4 in the serum during the four stages of healthy pregnancy. There were also markedly differences in the mRNA expression of 5-LO in the decidua during the two stages of healthy pregnancy. After pregnancy, the concentration of LTB4 in the serum of the subjects in early pregnancy and second trimester decreased, while in third trimester, its level increased. And after pregnancy, its level decreased. The 5-LO mRNA expression in the decidua in third trimester was obviously higher than the early pregnancy group.
Conclusions
The changes of LTB4 concentration in serum and the mRNA expression level of 5-LO in decidua may play an important role in the success and maintenance of a healthy pregnancy.
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References
Trowsdale J, Betz AG (2006) Mother’s little helpers: mechanisms of maternal-fetal tolerance. Nat Immunol 7(3):241–246
Peters-Golden M, Henderson WR Jr (2007) Leukotrienes. N Engl J Med 357:1841–1854
Hedi H, Norbert G (2004) 5-Lipoxygenase pathway, dendritic cells, and adaptive immunity. J Biomed Biotecnol 2:99–105
Samuelsson B, Dahlen SE, Lindgren JA et al (1987) Leukotrienes and lipoxins: structures, biosynthesis, and biological effects. Science 237(4819):1171–1176
Lewis RA, Austen KF, Soberman RJ (1990) Leukotrienes and other products of the 5-lipoxygenase pathway. Biochemistry and relation to pathobiology in human diseases. N Engl J Med 323(10):645–655
Gelfand EW, Dakhama A (2006) CD8+ T lymphocytes and leukotriene B4: novel interactions in the persistence and progression of asthma. J Allergy Clin Immunol 117(3):577–582
Chen H, Qin J, Wei P et al (2009) Effects of leukotriene B4 and prostaglandin E2 on the differentiation of murine Foxp3+ T regulatory cells and Th17 cells. Prostaglandins Leukot Essent Fatty Acids 80(4):195–200
Kihara Y, Yokomizo T, Kunita A et al (2010) The leukotriene B4 receptor, BLT1, is required for the induction of experimental autoimmune encephalomyelitis. Biochem Biophys Res Commun 394(3):673–678
Toda A, Terawaki K, Yamazaki S et al (2010) Attenuated Th1 induction by dendritic cells from mice deficient in the leukotriene B4 receptor 1. Biochimie 92(6):682–691
Romero R (2005) Novel aspects of neutrophil biology in human pregnancy. Am J Reprod Immunol 53:275
Mor G (2008) Inflammation and pregnancy: the role of toll-like receptors in trophoblast-immune interaction. Ann N Y Acad Sci 1127:121–128
Mor G, Cardenas I (2010) The immune system in pregnancy: a unique complexity. Am J Reprod Immunol 63(6):425–433
Dekel N, Gnainsky Y, Granot I et al (2010) Inflammation and implantation. Am J Reprod Immunol 63:17–21
Romero R, Espinoza J, Kusanovic JP et al (2006) The preterm parturition syndrome. BJOG 113(3):17–42
Romero R, Espinoza J, Goncalves LF et al (2006) Inflammation in preterm and term labour and delivery. Semin Fetal Neonatal Med 11:317–326
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Conflict of interest
This work was supported by grants from the National Natural Science Foundation of China (81070504/H0420).
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Jian, F., Ma, Y., Liu, Z. et al. The change of LTB4 and 5-LO during pregnancy. Arch Gynecol Obstet 288, 769–773 (2013). https://doi.org/10.1007/s00404-013-2823-y
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DOI: https://doi.org/10.1007/s00404-013-2823-y