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Characterization and antioxidant activities of marine pepsin soluble collagen from the skin of yellow goosefish Lophius litulon

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Abstract

Characteristics and antioxidant activities of pepsin-soluble collagen (PSC) from yellow goosefish (Lophius litulon) skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC’s denaturation temperature was ~17.56°C and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH (1–6) or low NaCl concentrations (≤2%). PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.

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Authors and Affiliations

  1. Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, 316000, China

    Bin Zheng  (郑斌), **ngwei **ang  (相兴伟), Hongyu Luo  (罗红宇) & Zhengshun Wen  (闻**顺)

  2. Zhejiang Marine Development Research Institute, Zhoushan, 316000, China

    Bin Zheng  (郑斌), **ngwei **ang  (相兴伟), Yufang Zhou  (周宇芳), Huicheng Yang  (杨会成) & Miaofei Liao  (廖妙飞)

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  1. Bin Zheng  (郑斌)
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  2. **ngwei **ang  (相兴伟)
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  3. Yufang Zhou  (周宇芳)
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  4. Huicheng Yang  (杨会成)
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  5. Hongyu Luo  (罗红宇)
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  6. Miaofei Liao  (廖妙飞)
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  7. Zhengshun Wen  (闻**顺)
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Correspondence to **ngwei **ang  (相兴伟).

Additional information

Supported by the International S&T Cooperation Program of China (No. 2015DFA30980), the Zhejiang Provincial Natural Science Foundation of China (No. LQ14C170001), and the Special Program for the Science and Technology Plan of Zhejiang Province (No. 2011C02003)

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Zheng, B., **ang, X., Zhou, Y. et al. Characterization and antioxidant activities of marine pepsin soluble collagen from the skin of yellow goosefish Lophius litulon . Chin. J. Ocean. Limnol. 35, 634–644 (2017). https://doi.org/10.1007/s00343-017-5377-1

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  • DOI: https://doi.org/10.1007/s00343-017-5377-1

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