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Hyperaccumulation of arsenic by callus, sporophytes and gametophytes of Pteris vittata cultured in vitro

  • Biotic and Abiotic Stress
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Abstract

The callus of Pteris vittata was induced from gametophytes generated from spores in vitro, and grew rapidly with periodical medium change. Arsenic tolerance and accumulation of P. vittata callus were compared with those of Arabidopsis thaliana callus. Cell death was not detected in P. vittata callus even at arsenate concentrations up to 2 mM; however, A. thaliana callus died at low (0.2 mM) arsenate concentrations. Meanwhile, P. vittata callus accumulated almost three times more As than A. thaliana callus when exposed to 0.2 mM arsenate. About 60% of the total As was removed when 7.5 g of P. vittata callus was cultured on 150 ml of half-strength MS liquid medium containing 450 μg As for 2 days. Furthermore, P. vittata callus, sporophytes, and gametophytes all grew well under 1 mM of arsenate and accumulated 1,250; 1,150 and 2,180 mg kg−1 dry weight As when grown on 2 mM arsenate for 15 or 30 days. The characteristics of non-differentiated cells, large biomass, ease of culture, good synchronization, and excellent As sequestering, make the callus of P. vittata a new ideal system to study the mechanisms of As hyperaccumulation and phytoremediation in As-contaminated groundwater.

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Abbreviations

As:

Arsenic

MS:

Murashige and Skoog

DW:

Dry weight

FW:

Fresh weight

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Acknowledgments

This work was supported by the National Science Foundation Council of China (Grant no. 30370127, 30500307 and 30670171).

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Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Bei**g, 100093, People’s Republic of China

    Xuexi Yang, Hui Chen, Wenzhong Xu, Zhenyan He & Mi Ma

  2. The Graduate School of Chinese Academy of Sciences, Bei**g, 100039, People’s Republic of China

    Xuexi Yang

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  1. Xuexi Yang
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  2. Hui Chen
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  3. Wenzhong Xu
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  4. Zhenyan He
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Correspondence to Mi Ma.

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Communicated by L. Peña.

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Yang, X., Chen, H., Xu, W. et al. Hyperaccumulation of arsenic by callus, sporophytes and gametophytes of Pteris vittata cultured in vitro . Plant Cell Rep 26, 1889–1897 (2007). https://doi.org/10.1007/s00299-007-0388-6

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