Abstract
Drug resistance to streptomycin in the clinical isolates of Mycobacterium tuberculosis (MTB) needs special consideration. It can mostly be caused by mutations in four genes with the names rpsL, rrs, gidB, and whiB7. The main objective of this study was the evaluation of the type and frequency of mutations in these mentioned genes using the PCR-sequencing method. This study was performed on 15 streptomycin-resistant and five streptomycin-sensitive isolates. Among resistant isolates, 11 samples contained mutations in codon 43 of the rpsL gene, which caused the lysine to be converted to arginine. Additionally, all of the isolates had mutations in the gidB. Missense mutations in codons 92 and 20 of this gene result in the amino acids Glutamic acid or Arginine being changed to Aspartic acid or Proline, respectively. No mutations in the rrs or whiB7 were found in any of the samples. Simultaneous mutations of rpsL and gidB were found in 10 isolates, the majority of which were Bei**g strain. The results showed that the mutations of rpsL and gidB genes are mostly responsible for the streptomycin resistance in the evaluated MTB isolates. Furthermore, the discovery of dual mutations in Bei**g strains highlights the strain's considerable potential for develo** Tuberculosis drug resistance.
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Acknowledgements
We thank the staff of the Department of Microbiology, which belonged to Golestan University of Medical Sciences, for their kind cooperation.
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The work was supported by Golestan University of Medical Sciences, Gorgan, Iran (grant number: IR.GOUMS.111209).
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(i) The conception or design of the study: EAG, HS, MS. (ii) the acquisition: MS, HS, SZ, KG analysis: HS, MS interpretation of the data: HS, MS, SG, AM, PMK; and (iii) writing of the manuscript: HS, MS, EAG. All authors approved the final version.
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Shafipour, M., Shirzad-Aski, H., Mohammadzadeh, A. et al. Evaluation of Mutations Related to Streptomycin Resistance in Mycobacterium tuberculosis Clinical Isolates. Curr Microbiol 79, 343 (2022). https://doi.org/10.1007/s00284-022-03043-9
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DOI: https://doi.org/10.1007/s00284-022-03043-9