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Mycobacterium tuberculosis ty** using Allele-specific oligonucleotide multiplex PCR (ASO–PCR) method

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Abstract

Mycobacterium tuberculosis (M. tuberculosis) genoty** provides valuable information related to the origin and the evolution of the isolates. This study aimed to evaluate the applicability of single-nucleotide polymorphisms (SNPs) technique for lineages identification of M. tuberculosis and compare it with mycobacterial interspersed repetitive units–variable number of tandem repeats (MIRU–VNTR) method. The lineages of 162 clinically isolates were evaluated using six pair primers by Multiplex-PCR based on SNPs. Among 162 isolates, 70 (43.2%) isolates were lineage 4, following that 62 (38.3%) and 22 (13.6%) isolates were lineage 3 and 2, respectively. The method could not type 8 (4.9%) isolates. Moreover, we could identify 71 out of 79 unknown isolates resulted from the MIRU–VNTR method. The results showed that the SNP ty** method has the potential to determine the lineages of M. tuberculosis as a rapid laboratory screening test.

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The raw PCR pictures will be available upon request.

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Acknowledgements

We thank the staff of the Tuberculosis laboratory of Health care Center, belonged to Golestan University of Medical Sciences, for their kind cooperation.

Funding

The work was supported by Golestan University of Medical Sciences, Gorgan, Iran (grant number: IRGOUMS110702).

Author information

Authors and Affiliations

  1. Infectious Diseases Research Center, Golestan University of Medical Sciences, 49178-67439, Gorgan, Iran

    Maryam Shafipour, Hesamaddin Shirzad-Aski, Ezzat Allah Ghaemi, Ahmad Sohrabi, Maya Babaii Kochaksaraei & Basireh Baei

  2. Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran

    Maryam Shafipour

  3. Cancer Control Research Center, Cancer Control Foundation, Iran University of Medical Sciences, Tehran, Iran

    Ahmad Sohrabi

  4. Tuberculosis Laboratory of Health Care Center, Golestan University of Medical Sciences, Gorgan, Iran

    Masoume Taziki & Somayeh Rahimi

  5. Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Kiarash Ghazvini

Authors
  1. Maryam Shafipour
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  2. Hesamaddin Shirzad-Aski
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  3. Ezzat Allah Ghaemi
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  4. Ahmad Sohrabi
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  5. Maya Babaii Kochaksaraei
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  6. Masoume Taziki
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  7. Somayeh Rahimi
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  8. Kiarash Ghazvini
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  9. Basireh Baei
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Contributions

Conceptualization: HS, MS, EAG. Funding: HS. Resources: MS, EAG, KG. Development and implementation: MBK, MT, SR, BB. Investigation: MS, HS, MT, MBK. Statistical analysis: AS. Writing: HS, MS, EAG. All authors approved the final version.

Corresponding author

Correspondence to Hesamaddin Shirzad-Aski.

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Conflict of interest

The authors have no conflicts of interest to declare for this study.

Ethical Approval

The ethics committee of the Golestan University of Medical Science, Iran, reviewed and approved the study (ethical approval code: IR.GOUMS.REC.1397.300).

Consent to Participate

All the required information was anonymized and the names of patients were de-identified. Samples were obtained with the consent of the patient to identify and treat TB.

Research Involving Animal Participants

Animals, biological materials, plants, algae, and fungi were not used in this study.

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Shafipour, M., Shirzad-Aski, H., Ghaemi, E.A. et al. Mycobacterium tuberculosis ty** using Allele-specific oligonucleotide multiplex PCR (ASO–PCR) method. Curr Microbiol 78, 4009–4013 (2021). https://doi.org/10.1007/s00284-021-02659-7

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  • DOI: https://doi.org/10.1007/s00284-021-02659-7

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