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Rhizobium terrae sp. nov., Isolated from an Oil-Contaminated Soil in China

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A Gram-stain-negative, facultative aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, designated strain NAU-18T was isolated from an oil-contaminated soil in China. Strain NAU-18T could grow at 10–42 °C (optimum, 30 °C), at pH 5.0–8.0 (optimum, 7.0) and in the presence of 0–2.0% (w/v) NaCl (optimum, 0.5% NaCl in R2A). The predominant fatty acids were C18:1ω7c (71.2%) and Summed feature 2 (5.1%), representing 76.3% of the total fatty acids. The major respiratory quinones were Q9 and Q10. The DNA G + C content of strain NAU-18T was 61.4 mol% based on its draft genome sequence. Genome annotation of strain NAU-18T predicted the presence of 6668 genes, of which 6588 are coding proteins and 80 are RNA genes. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NAU-18T was a member of the genus Rhizobium and showed 96.93% (with 93.2% coverage) and 96.81% (with 100% coverage) identities with those of Neorhizobium alkalisoli CCBAU 01393T and Rhizobium oryzicola ZYY136T, respectively. In the phylogenetic analysis, strain NAU-18T and R. oryzicola ZYY136T are consistently placed in the same branch. Strain NAU-18T represents a novel species within the genus Rhizobium, for which the name Rhizobium terrae sp. nov. is proposed, with the type strain NAU-18T (=KCTC 62418T = CCTCC AB 2018075T).

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Authors and Affiliations

  1. Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nan**g Agricultural University, Nan**g, 210095, China

    Zhe-pu Ruan, Wei-miao Cao, ** Zhang, **g-tian-yi Liu, Jian-chun Zhu & Jian-dong Jiang

  2. Experimental Biology Education Center, College of Life Sciences, Nan**g Agricultural University, Nan**g, 210095, China

    Jian-chun Zhu & Bing Hu

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  1. Zhe-pu Ruan
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The GenBank/EMBL/DDBJ accession numbers for the draft genome sequence and 16S rRNA gene, atpD and recA gene sequence of strain NAU-18T are QJKM00000000, MH244125, MH327782 and MH327781, respectively. The annotation information is deposited at the Joint Genomic Institute (JGI), with IMG genome ID 2784132094.

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Ruan, Zp., Cao, Wm., Zhang, X. et al. Rhizobium terrae sp. nov., Isolated from an Oil-Contaminated Soil in China. Curr Microbiol 77, 1117–1124 (2020). https://doi.org/10.1007/s00284-020-01889-5

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