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The Draft Genome Sequence of a Novel High-Efficient Butanol-Producing Bacterium Clostridium Diolis Strain WST

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Abstract

A wild-type solventogenic strain Clostridium diolis WST, isolated from mangrove sediments, was characterized to produce high amount of butanol and acetone with negligible level of ethanol and acids from glucose via a unique acetone-butanol (AB) fermentation pathway. Through the genomic sequencing, the assembled draft genome of strain WST is calculated to be 5.85 Mb with a GC content of 29.69% and contains 5263 genes that contribute to the annotation of 5049 protein-coding sequences. Within these annotated genes, the butanol dehydrogenase gene (bdh) was determined to be in a higher amount from strain WST compared to other Clostridial strains, which is positively related to its high-efficient production of butanol. Therefore, we present a draft genome sequence analysis of strain WST in this article that should facilitate to further understand the solventogenic mechanism of this special microorganism.

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Acknowledgements

This work was financially supported the “Sail Plan” Program for the Introduction of Outstanding Talents of Guangdong Province of China (No. 14600601), the Major University Research Foundation of Guangdong Province of China (No. 2015KQNCX041), the Start-Up Funding of Shantou University (No. NTF15007), the International Cooperation Research Project of Shantou University (No. NC2017001) and the Foundation of Guangdong Provincial Key Laboratory of Marine Biotechnology (No. GPKLMB201702).

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Authors and Affiliations

  1. Department of Biology, Shantou University, Shantou, 515063, Guangdong, China

    Chaoyang Chen, Chongran Sun & Yi-Rui Wu

  2. STU-UNIVPM Joint Algal Research Center, Shantou University, Shantou, 515063, Guangdong, China

    Yi-Rui Wu

  3. Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, Guangdong, China

    Yi-Rui Wu

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  1. Chaoyang Chen
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  2. Chongran Sun
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  3. Yi-Rui Wu
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Correspondence to Yi-Rui Wu.

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The authors have declared there was no conflict of interest.

Additional information

Nucleotide Sequence Accession Numbers The draft genomic sequence of C. diolis strain WST has been deposited into GenBank with the accession number of PRKY00000000.1.

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Chen, C., Sun, C. & Wu, YR. The Draft Genome Sequence of a Novel High-Efficient Butanol-Producing Bacterium Clostridium Diolis Strain WST. Curr Microbiol 75, 1011–1015 (2018). https://doi.org/10.1007/s00284-018-1481-5

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  • DOI: https://doi.org/10.1007/s00284-018-1481-5

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