Abstract
The demand for 1,3-propanediol (1,3-PDO) has increased sharply due to its role as a monomer for the synthesis of polytrimethylene terephthalate (PTT). Although Clostridium butyricum is considered to be one of the most promising bioproducers for 1,3-PDO, its low productivity hinders its application on industrial scale because of the longer time needed for anaerobic cultivation. In this study, an excellent C. butyricum (DL07) strain was obtained with high-level titer and productivity of 1,3-PDO, i.e., 104.8 g/L and 3.38 g/(L•h) vs. 94.2 g/L and 3.04 g/(L•h) using pure or crude glycerol as substrate in fed-batch fermentation, respectively. Furthermore, a novel sequential fed-batch fermentation was investigated, in which the next bioreactor was inoculated by C. butyricum DL07 cells growing at exponential phase in the prior bioreactor. It could run steadily for at least eight cycles. The average concentration of 1,3-PDO in eight cycles was 85 g/L with the average productivity of 3.1 g/(L•h). The sequential fed-batch fermentation could achieve semi-continuous production of 1,3-PDO with higher productivity than repeated fed-batch fermentation and would greatly contribute to the industrial production of 1,3-PDO by C. butyricum.
Key points
• A novel C. butyricum strain was screened to produce 104.8 g/L 1,3-PDO from glycerol.
• Corn steep liquor powder was used as a cheap nitrogen source for 1,3-PDO production.
• A sequential fed-batch fermentation process was established for 1,3-PDO production.
• An automatic glycerol feeding strategy was applied in the production of 1,3-PDO.
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Funding
This work was supported by the National Natural Science Foundation of China (Grant 521 No. 21476042, awarded to Zhi-Long **u) and the Fundamental Research Funds for the Central Universities 522 (Grant No. DUT17ZD209, awarded to Ya-Qin Sun).
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ZLX and XLW contributed conception and design of the study. XLW, JJZ, JTS, and YFZ performed the experiments. XLW, JJZ, and YQS analyzed the data. XLW wrote the manuscript. All authors contributed to manuscript revision, read, and approved the submitted version.
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Wang, XL., Zhou, JJ., Shen, JT. et al. Sequential fed-batch fermentation of 1,3-propanediol from glycerol by Clostridium butyricum DL07. Appl Microbiol Biotechnol 104, 9179–9191 (2020). https://doi.org/10.1007/s00253-020-10931-2
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DOI: https://doi.org/10.1007/s00253-020-10931-2