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The binding characters study of lead removal by Lactobacillus plantarum CCFM8661

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Abstract

The dietary intake of lead (Pb) via contaminated food and drinking water possesses a serious risk to human health. In this study, the Pb2+ binding ability of 25 lactic acid bacteria strains was investigated, and the strain with the highest binding property, Lactobacillus plantarum CCFM8661 (36.66 ± 1.01 %), was selected for further study. The results of electron microscope revealed that most of Pb2+ was bound on the surface of bacterial cells and small amount of Pb2+ was observed in the cells. The comparison of the binding ability of different cellular components also demonstrated that the cell walls (73.79 ± 0.31 %) play a significant role in the Pb2+ binding. Through the study of the binary metals systems, the results showed that L. plantarum CCFM8661 still preferred to bind Pb2+ at the presence of other cations such as Fe2+ and Zn2+. The binding process was well explained by the Langmuir isotherm model (R 2 = 0.97), the Langmuir–Freundlich dual isotherm model (R 2 = 0.99), and the pseudo-second-order kinetic model (R 2 = 0.99).

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Acknowledgments

This work was supported by the National Natural Science Foundation of China Key Program (No. 31530056), the National Science Fund for Distinguished Young Scholars (No. 31125021), the National Natural Science Foundation of China General Program (No. 31470161), the Science and Nature Foundation of Jiangsu Province (No. BK 20131102), the key projects in the National Science & Technology Pillar Program during the twelfth five-year plan period (Nos. 2012BAD12B02, 2012BAD28B08), the National Basic Research Program of China (973 Program No. 2012CB720802), the 111 Project B07019, and the Program for Changjiang Scholars and Innovative Research Team in University (IRT1249) and Young Foundation of Jiangnan University (No. JUSRP115A23). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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    Authors and Affiliations

    1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People’s Republic of China

      Ruijie Yin, Qixiao Zhai, Leilei Yu, Yue **ao, Gang Wang, Ruipeng Yu, Fengwei Tian & Wei Chen

    2. Bei**g Innovation Centre of Food Nutrition and Human Health, Bei**g Technology and Business University, Bei**g, 100048, People’s Republic of China

      Wei Chen

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    1. Ruijie Yin
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    Correspondence to Fengwei Tian or Wei Chen.

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    Ruijie Yin and Qixiao Zhai have contributed equally to this work.

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    Yin, R., Zhai, Q., Yu, L. et al. The binding characters study of lead removal by Lactobacillus plantarum CCFM8661. Eur Food Res Technol 242, 1621–1629 (2016). https://doi.org/10.1007/s00217-016-2661-9

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    • DOI: https://doi.org/10.1007/s00217-016-2661-9

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