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A biochemical system of rapidly detecting bacteria based on ATP bioluminescence technology

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Abstract

A biochemical detection system based on ATP bioluminescence which can detect bacteria and microorganisms automatically and rapidly is proposed. Meanwhile, reagent formulations are optimized. In the detecting system, weak fluorescence loss is calibrated, and receiving efficiency of the photomultiplier tube is improved by making use of optical unit. The results show that rapid detection accuracy can reach 86 % compared with the traditional plate-counting method, the detecting time is controlled within twenty minutes, and detectable ATP concentration range achieves 10−15–10−8 M. Moreover, the series of curves obtained by measurements for the common variety of food (drinks, meat etc.) show that the device has a good performance and gets satisfactory testing results.

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Acknowledgments

This paper was supported by the State Natural Sciences Foundation of China (No. 10874103).

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Authors and Affiliations

  1. College of Physics and Electronics, Shandong Normal University, **an, 250014, People’s Republic of China

    Chunxing Wang, Bin Zhang & **aomei Zhuang

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  1. Chunxing Wang
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  2. Bin Zhang
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  3. **aomei Zhuang
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Correspondence to Chunxing Wang.

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Wang, C., Zhang, B. & Zhuang, X. A biochemical system of rapidly detecting bacteria based on ATP bioluminescence technology. Eur Food Res Technol 236, 41–46 (2013). https://doi.org/10.1007/s00217-012-1854-0

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  • DOI: https://doi.org/10.1007/s00217-012-1854-0

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