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A rapid quantitative method for polysaccharides in green tea and oolong tea

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Abstract

Tea polysaccharides (TPS) from five different kinds of tea contain similar composition of sugars and amino acids. Polysaccharide part of TPS is mainly composed of arabinose, galactose and glucose with the approximate molar ratio of 1:1:0.5. Protein part of TPS is composed of 16 normal amino acids among which Val, Ala, Gly, Glu are the major proportion. Based on the results, the purified TPS was used as the calibration standard in phenol sulfuric acid reaction for the calibration curve that was constructed by plotting absorbance versus mass of TPS with a correlation coefficient of 0.9961. In the developed method, TPS mass recovery ranges from 84.88 to 95.7% with a relative standard deviation of 5.53%. It is found that the developed method is simple, rapid, reliable and is ideally suitable for quantifying TPS from green tea or oolong tea.

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Abbreviations

TPS:

Tea polysaccharides

HPGPC:

High-performance gel-permeation chromatography

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Acknowledgments

This research was supported by Natural Scientific Foundation of Shandong (China) (Z 2004D05).

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Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, People’s Republic of China

    Dongfeng Wang, **aoling Zhou, Lei Li, Yangfeng Hou, Jipeng Sun & **gfeng Wang

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  1. Dongfeng Wang
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  2. **aoling Zhou
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  3. Lei Li
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  4. Yangfeng Hou
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  5. Jipeng Sun
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  6. **gfeng Wang
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Correspondence to Dongfeng Wang.

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Wang, D., Zhou, X., Li, L. et al. A rapid quantitative method for polysaccharides in green tea and oolong tea. Eur Food Res Technol 226, 691–696 (2008). https://doi.org/10.1007/s00217-007-0578-z

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  • DOI: https://doi.org/10.1007/s00217-007-0578-z

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