SpringerLink
Log in

Simple and rapid detection of tyrosinase activity with the adjustable light scattering properties of CoOOH nanoflakes

  • Research Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Tyrosinase (TYR), as an important biological enzyme, has been widely used in synthetic biology, medical hairdressing, environmental detection, biological sensors, and other fields. In clinical practice, tyrosinase activity is an important indicator for detecting melanoma. Therefore, the detection of tyrosinase activity is of great importance. Based on the polyphenol oxidase activity of tyrosinase, a simple and rapid detection method was proposed based on the adjustable light scattering properties of cobalt hydroxyl oxide nanoflakes (CoOOH NFs). It was found that the amount and size of CoOOH NFs decreased due to the redox reaction mediated by catechol (CC), resulting in a lower light scattering signal of CoOOH NFs. However, in the presence of tyrosinase, catechol was oxidized to a quinone structure, resulting in the reduced decomposition of CoOOH NFs and recovered light scattering signal, which was developed for the quantitative detection of tyrosinase activity. It was found that in the range of 10–400 U/L, the light scattering intensity was correlated linearly with tyrosinase activity, and the limit of detection was 6.71 U/L (3σ/k). To verify the feasibility of the proposed method in clinical samples, the spiked recovery experiments were carried out with human serum samples, which showed recovery rates between 93.0% and 104.6%, suggesting the high accuracy. The proposed assay provides a simple and rapid method for detection of a natural enzyme based on the adjustable light scattering properties of CoOOH nanoflakes, which lays the foundation for the development of various enzyme sensing applications in the future.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1.
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Abbreviations

UCNPs:

Upconversion nanoparticles

NPs:

Nanoparticles

APBA-QDs:

3-Aminophenyl boric acid-functionalized quantum dots

PDA:

Polydopamine

References

  1. Noh H, Lee SJ, Jo HJ, Choi HW, Hong S, Kong KH. Histidine residues at the copper-binding site in human tyrosinase are essential for its catalytic activities. J Enzyme Inhib Med Chem. 2020;35:726–32.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Rolff M, Schottenheim J, Decker H, Tuczek F. Copper-O2 reactivity of tyrosinase models towards exteRNAl monophenolic substrates: molecular mechanism and comparison with the enzyme. Chem Soc Rev. 2011;40:4077–98.

    Article  CAS  PubMed  Google Scholar 

  3. Loizzo MR, Tundis R, Menichini F. Natural and synthetic tyrosinase inhibitors as antibrowning agents: an update. Compr Rev Food Sci Food Saf. 2012;11:378–98.

    Article  CAS  Google Scholar 

  4. Li XQ, Li SQ, Liang XP, McClements DJ, Liu XB, Liu FG. Applications of oxidases in modification of food molecules and colloidal systems: laccase, peroxidase and tyrosinase. Trends Food Sci Tech. 2020;103:78–93.

    Article  CAS  Google Scholar 

  5. Yu FF, Lu YS, Zhong ZM, Qu BL, Wang MF, Yu XY, Chen JY. Mitf involved in innate immunity by activating tyrosinase-mediated melanin synthesis in pteria penguin. Front Immunol. 2021;12: 626493.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Jantakee K, Tragoolpua Y. Activities of different types of Thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals. Biol Res. 2015;48:4.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Odonbayar B, Murata T, Batkhuu J, Yasunaga K, Goto R, Sasakit K. Antioxidant flavonols and phenolic compounds from atraphaxis frutescens and their inhibitory activities against insect phenoloxidase and mushroom tyrosinase. J Nat Prod. 2016;79:3065–71.

    Article  CAS  PubMed  Google Scholar 

  8. Pu YY, Zhou BG, **ang HJ, Wu WC, Yin HH, Yue WW, Yin YF, Li HY, Chen Y, Xu HX. Tyrosinase-activated prodrug nanomedicine as oxidative stress amplifier for melanoma-specific treatment. Biomaterials. 2020;259: 120329.

    Article  CAS  PubMed  Google Scholar 

  9. Niu C, Aisa HA. Upregulation of melanogenesis and tyrosinase activity: potential agents for vitiligo. Molecules. 2017;22:1303.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Arrowitz C, Schoelermann AM, Mann T, Jiang LI, Weber T, Kolbe L. Effective tyrosinase inhibition by thiamidol results in significant improvement of mild to moderate melasma. J Invest Dermatol. 2019;139:1691–8.

    Article  CAS  PubMed  Google Scholar 

  11. Yang S, Jiang JX, Zhou AX, Zhou YB, Ye WL, Cao DS, Yang RH. Substrate-photocaged enzymatic fluorogenic probe enabling sequential activation for light-controllable monitoring of intracellular tyrosinase activity. Anal Chem. 2020;92:7194–9.

    Article  CAS  PubMed  Google Scholar 

  12. Wu XF, Li LH, Shi W, Gong QY, Ma HM. Near-infrared fluorescent probe with new recognition moiety for specific detection of tyrosinase activity: design, synthesis, and application in living cells and zebrafish. Angew Chem-In Ed. 2016;55:14728–32.

    Article  CAS  Google Scholar 

  13. Li SQ, Liu D, Wu BY, Sun HP, Liu XY, Zhang HX, Ding NN, Wu L. One-pot synthesis of a peroxidase-like nanozyme and its application in visual assay for tyrosinase activity. Talanta. 2022;239: 123088.

    Article  CAS  PubMed  Google Scholar 

  14. Li J, Wei YY, Liu XP, Xu ZR. Plasmonic photothermal biosensor for visual detection of tyrosinase and dopamine based on manganese dioxide nanosheets-mediated etching of gold nanorods. Sensor Actuat B- Chem. 2022;353: 131139.

    Article  CAS  Google Scholar 

  15. Shelef O, Sedgwick AC, Pozzi S, Green O, Satchi-Fainaro R, Shabat D, Sessler JL. Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols. Chem Commun. 2021;57:11386–9.

    Article  CAS  Google Scholar 

  16. Erkmen C, Demir Y, Kurbanoglu S, Uslu B. Multi-purpose electrochemical tyrosinase nanobiosensor based on poly(3,4 ethylenedioxythiophene) nanoparticles decorated graphene quantum dots: applications to hormone drugs analyses and inhibition studies. Sensor Actuat B-Chem. 2021;343: 130164.

    Article  CAS  Google Scholar 

  17. Zhuang XM, Hu YJ, Wang JJ, Hu JY, Wang Q, Yu XX. A colorimetric and SERS dual-readout sensor for sensitive detection of tyrosinase activity based on 4-mercaptophenyl boronic acid modified AuNPs. Anal Chim Acta. 2021;1188: 339172.

    Article  CAS  PubMed  Google Scholar 

  18. Yildiz HB, Freeman R, Gill R, Willner I. Electrochemical, photoelectrochemical, and piezoelectric analysis of tyrosinase activity by functionalized nanoparticles. Anal Chem. 2008;80:2811–6.

    Article  CAS  PubMed  Google Scholar 

  19. Munyemana JC, Chen J, Tang H, Han YX, Wang JJ, Qiu HD. Discriminative detection of dopamine and tyrosinase based on polydopamine dots triggered by Fenton-like activity of Mn3O4 nanoparticles. ACS Appl Nano Mater. 2021;4:2820–7.

    Article  CAS  Google Scholar 

  20. Lemineur JF, Stockmann TJ, Medard J, Smadja C, Combellas C, Kanoufi F. Optical nanoimpacts of dielectric and metallic nanoparticles on gold surface by reflectance microscopy: adsorption or bouncing? J Anal Test. 2019;3:175–88.

    Article  Google Scholar 

  21. Mao ZY, Zhu LN, Gao J, Liu JJ, Wei YH, Li XY, Yin BC, Wang J. A CoOOH nanoflake-based light scattering probe for the simple and selective detection of uric acid in human serum. Anal Methods. 2018;10:4951–7.

    Article  CAS  Google Scholar 

  22. Wei YH, Li XY, Gao J, Liu JJ, Yuan D, Yin BC, Wang J. Size-dependent modulation of CoOOH nanoflakes light scattering for rapid and selective detection of tetracycline in milk. J Anal Test. 2018;2:332–41.

    Article  Google Scholar 

  23. Zhu LN, Cheng R, Kang KW, Chen MY, Zhan T, Wang J. Size-dependent light scattering of CoOOH nanoflakes for convenient and sensitive detection of alkaline phosphatase in human serum. Luminescence. 2021;36:1317–26.

    Article  CAS  PubMed  Google Scholar 

  24. Li ZM, Zhong XL, Wen SH, Zhang L, Liang RP, Qiu JD. Colorimetric detection of methyltransferase activity based on the enhancement of CoOOH nanozyme activity by ssdna. Sensor Actuat B- Chem. 2019;281:1073–9.

    Article  CAS  Google Scholar 

  25. Wu ZH, Nan DY, Yang H, Pan S, Liu H, Hu XL. A ratiometric fluorescence-scattered light strategy based on MoS2 quantum dots/CoOOH nanoflakes system for ascorbic acid detection. Anal Chim Acta. 2019;1091:59–68.

    Article  CAS  PubMed  Google Scholar 

  26. Prexler SM, Frassek M, Moerschbacher BM, Dirks-Hofmeister ME. Catechol oxidase versus tyrosinase classification revisited by site-directed mutagenesis studies. Angew Chem-In Ed. 2019;58:8757–61.

    Article  CAS  Google Scholar 

  27. Craig DB, Hiebert Z. Analysis of complexes of metabolites with europium tetracycline using capillary electrophoresis coupled with laser-induced luminescence detection. Biometals. 2017;30:449–58.

    Article  CAS  PubMed  Google Scholar 

  28. Chen Z, Wang X, Keßler S, Fan Q, Huang M, Cölfen H. Synthesis of hierarchical transition metal oxyhydroxides in aqueous solution at ambient temperature and their application as OER electrocatalysts. J Energy Chem. 2022;71:89–97.

    Article  CAS  Google Scholar 

  29. Li L, Wang C, Liu K, Wang Y, Liu K, Lin Y. Hexagonal cobalt oxyhydroxide–carbon dots hybridized surface: high sensitive fluorescence turn-on probe for monitoring of ascorbic acid in rat brain following brain ischemia. Anal Chem. 2015;87:3404–11.

    Article  CAS  PubMed  Google Scholar 

  30. Zou BQ, Zhang HZ, Fu Z, Zhan T, Wang J. Size-modulated optical property of gold nanorods for sensitive and colorimetric detection of thiourea in fruit juice. Talanta. 2021;225: 121965.

    Article  CAS  PubMed  Google Scholar 

  31. Liu JJ, Yan HH, Zhang Q, Gao PF, Li CM, Liang GL, Huang CZ, Wang J. High-resolution vertical polarization excited dark-field microscopic imaging of anisotropic gold nanorods for the sensitive detection and spatial imaging of intracellular microRNA-21. Anal Chem. 2020;92:13118–25.

    Article  CAS  PubMed  Google Scholar 

  32. Liu JJ, Wen S, Yan HH, Cheng R, Zhu F, Gao PF, Zou HY, Huang CZ, Wang J. The accurate imaging of collective gold nanorods with a polarization-dependent dark-field light scattering microscope. Anal Chem. 2023;95:1169–75.

    CAS  PubMed  Google Scholar 

  33. **a J, Yang Y, Jiang X, Shabbir M, Luo X. Cobalt oxyhydroxide nanoflakes/cellulose composite membranes with enhanced detection of ascorbic acid. ACS Appl Polym Mater. 2022;4:469–78.

    Article  CAS  Google Scholar 

  34. Munjal N, Sawhney SK. Stability and properties of mushroom tyrosinase entrapped in alginate, poyacrylamide and gelatin gels. Enzyme Microb Technol. 2002;30:613–9.

    Article  CAS  Google Scholar 

  35. Liu G, Zhao J, Lu S, Wang S, Sun J, Yang X. Polymethyldopa nanoparticles-based fluorescent sensor for detection of tyrosinase activity. ACS Sens. 2018;3:1855–62.

    Article  CAS  PubMed  Google Scholar 

  36. Cen Y, Tang J, Kong X-J, Wu S, Yuan J, Yu R-Q, Chu X. A cobalt oxyhydroxide-modified upconversion nanosystem for sensitive fluorescence sensing of ascorbic acid in human plasma. Nanoscale. 2015;7:13951–7.

    Article  CAS  PubMed  Google Scholar 

  37. Wang M, **e JL, Li J, Fan YY, Deng X, Duan HL, Zhang ZQ. 3-Aminophenyl boronic acid functionalized quantum-dot-based ratiometric fluorescence sensor for the highly sensitive detection of tyrosinase activity. ACS Sens. 2020;5:1634–40.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 22174115) and the Graduate Education and Teaching Reform Research Project of Chongqing (No. yjg223038).

This work contains human blood samples from volunteers at Southwest University, which operated according to the ethical standards of the Ethics Committee of Southwest University Hospital.

Author information

Authors and Affiliations

  1. Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, People’s Republic of China

    Meng **ao Li, Kai Wen Kang, Min Huang, Ru Cheng & Jian Wang

  2. The People’s Hospital of Qingdao West Coast New Area, Qingdao, Shandong, 266499, People’s Republic of China

    Wei Wang

  3. Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, 400067, People’s Republic of China

    Jie Gao

Authors
  1. Meng **ao Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kai Wen Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Min Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ru Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jie Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jian Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jian Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Published in the topical collection Young Investigators in (Bio-)Analytical Chemistry 2023 with guest editors Zhi-Yuan Gu, Beatriz Jurado-Sánchez, Thomas H. Linz, Leandro Wang Hantao, Nongnoot Wongkaew, and Peng Wu.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, M.X., Kang, K.W., Huang, M. et al. Simple and rapid detection of tyrosinase activity with the adjustable light scattering properties of CoOOH nanoflakes. Anal Bioanal Chem 415, 4569–4578 (2023). https://doi.org/10.1007/s00216-023-04710-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-023-04710-x

Keywords

Search

Navigation