SpringerLink
Log in

NanoSIMS50 — a powerful tool to elucidate cellular localization of halogenated organic compounds

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Persistent organic pollutants are widely distributed in the environment and lots of toxicological data are available. However, little is known on the intracellular fate of such compounds. Here a method applying secondary ion mass spectrometry is described that can be used to visualize cellular localization of halogenated compounds and to semi-quantitatively calculate concentrations of such compounds. Of the model compounds tested, TBBPA was homogenously distributed in the cell membrane of the H295R cells while PFOS accumulated in very distinct locations in the cell membrane. Relative intracellular concentrations of 4-OH-BDE69 and 4-OH-BDE121 in GH3.TRE were 61 % and 18 %, respectively, compared to the parent compounds. These differences may partly explain that observed effect concentrations for 4-OH-BDEs in in vitro experiments are usually lower than what would be expected based on receptor binding studies. NanoSIMS50 proved to be a powerful tool to describe the cellular distribution of halogenated compounds. The semi-quantitative data that can be obtained may help to further explain results from in vitro or in vivo experiments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Audinot JN, Senou M, Migeon H-N, Many M-C (2008) Visualization of thyroid hormone synthesis by ion imaging. Appl Surf Sci 255:1185–1189

    Article  CAS  Google Scholar 

  2. Audinot J-N, Cabin-Flaman A, Philipp P, Legent G, Wirtz T, Migeon H-N (2011) NanoSIMS50 imaging of thin samples coupled with neutral cesium deposition. Surf Interface Anal 43:302–305

    Article  CAS  Google Scholar 

  3. Bourez S, Le Lay S, van den Daelen C, Louis C, Larondelle Y, Thomé J-P, Schneider YJ, Dugail I, Debier C (2012) Accumulation of polychlorinated biphenyls in adipocytes: selective targeting to lipid droplets and role of caveolin-1. PLoS One 7:e31834

    Article  CAS  Google Scholar 

  4. Eybe T, Audinot J-N, Bohn T, Guignard C, Migeon H-N, Hoffmann L (2008) NanoSIMS 50 elucidation of the natural element composition in structures of cyanobacteria and their exposure to halogen compounds. J Appl Microbiol 105:1502–1510

    Article  CAS  Google Scholar 

  5. Eybe T, Bohn T, Audinot J-N, Udelhoven T, Cauchie HM, Migeon H-N, Hoffmann L (2009) Uptake visualization of deltamethrin by nanoSIMS and acute toxicity to the water flea Daphnia magna. Chemosphere 76:134–140

    Article  CAS  Google Scholar 

  6. Freitas J, Cano P, Craig-Veith C, Goodson ML, Furlow JD, Murk AJ (2011) Detection of thyroid hormone receptor disruptors by a novel stable in vitro reporter gene assay. Toxicol in vitro 25:257–266

    Article  CAS  Google Scholar 

  7. Gracia T, Hilscherova K, Jones PD, Newsted JL, Zhang X, Hecker M, Higley EB, Sanderson JT, Yu RM, Wu RS, Giesy JP (2006) The H295R system for evaluation of endocrine-disrupting effects. Ecotoxicol Environ Saf 65:265–305

    Article  Google Scholar 

  8. Gutleb AC, Meerts IATM, Bergsma JH, Schriks M, Murk AJ (2005) T-screen as a tool to identify thyroid hormone receptor active compounds. Environ Toxicol Pharmacol 19:231–238

    Article  CAS  Google Scholar 

  9. Gutleb AC, Cenijn P, van Velzen M, Lie E, Ropstad E, Skaare JU, Malmberg T, Bergman Å, Gabrielsen GW, Legler J (2010) Complete saturation of thyroid hormone transport capacity of TTR in wild living polar bear (Ursus maritimus) by metabolites of PCBs. Environ Sci Technol 44:3149–3154

    Article  CAS  Google Scholar 

  10. Hecker M, Giesy JP (2008) Novel trends in endocrine disruptor testing: the H295R Steroidigenic Assay for identification of inducers and inhibitors of hormone production. Anal Bioanal Chem 390:287–291

    Article  CAS  Google Scholar 

  11. Hu W-Y, Jones PD, Upham BL, Trosko JE, Lau C, Giesy JP (2002) Inhibition of gap junctional intercellular communication by perfluorinated compounds in rat liver and dolphin kidney epithelial cell lines in vitro and Sprague Dawley rats in vivo. Toxicol Sci 68:429–436

    Article  CAS  Google Scholar 

  12. Hu W-Y, Jones PD, DeCoen W, King L, Fraker P, Newsted J, Giesy JP (2003) Alterations in cell membrane properties caused by perfluorinated compounds. Comp Biochem Physiol C 135:77–88

    Google Scholar 

  13. Kraugerud M, Zimmer KE, Ropstad E, Verhaegen S (2011) Perfluorinated compounds differentially affect steroidogenesis and viability in the human adrenocortical carcinoma (H295R) in vitro cell assay. Toxicol Lett 205:62–68

    Article  CAS  Google Scholar 

  14. Lechene C, Hillion F, McMahon G, Benson D, Kleinfeld AM, Kampf JP, Distel D, Luyten Y, Bonventre J, Hentschel D, Park KM, Ito S, Schwartz M, Benichou G, Slodzian G (2006) High resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry. J Biol 5:20

    Article  Google Scholar 

  15. Lehmler H-J, **e W, Bothun PM, Bummer PM, Knutson BL (2006) Mixing of perfluorooctanesulfonic acid (PFOS) potassium salt with dipalmitoyl phosphatidylcholine (DPCC). Colloids Surf B 51:25–29

    Article  CAS  Google Scholar 

  16. Letcher RJ, Bustnes JO, Dietz R, Jenssen BM, Jørgensen EH, Sonne C, Verreault J, Vijayan MM, Gabrielsen GW (2010) Exposure and effect assessment of persistent organohalogen contaminants in arctic wildlife and fish. Sci Total Environ 408:2995–3043

    Article  CAS  Google Scholar 

  17. Marsh G, Stenutz R, Bergman Å (2003) Synthesis of hydroxylated and methoxylated polybrominated diphenyl ethers – natural products and potential polybrominated diphenyl ether metabolites. Europ J Org Chem 14:2566–2576

    Article  Google Scholar 

  18. Montaño M, Zimmer KE, Dahl E, Berg V, Olsaker I, Skaare JU, Murk AJ, Ropstad E, Verhaegen S (2011) Effects of mixtures of persistent organic pollutants (POPs) derived from cod liver oil on H295R steroidogenesis. Food Chem Toxicol 49:2328–2335

    Article  Google Scholar 

  19. Oskam IC, Lyche JL, Krogenaes A, Thomassen R, Skaare JU, Wiger R, Dahl E, Sweeney T, Stien A, Ropstad E (2005) Effects of long-term maternal exposure to low doses of PCB126 and PCB153 on the reproductive system and related hormones of young male goats. Reproduction 130:731–742

    Article  CAS  Google Scholar 

  20. Rasband WS, ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://rsb.info.nih.gov/ij/, 1997–2008

  21. Scheringer M (2009) Long-range transport of organic chemicals in the environment. Environ Toxicol Chem 28:677–690

    Article  CAS  Google Scholar 

  22. Schriks M, Vrabie CM, Gutleb AC, Faassen EJ, Rietjens IMCM, Murk AJ (2006) T-screen to quantify functional potentiating, antagonistic and thyroid hormone-like activities of polyhalogenated aromatic hydrocarbons (PHAHs). Toxicol in vitro 20:490–498

    Article  CAS  Google Scholar 

  23. **e W, Ludewig G, Wang K, Lehmler H-J (2010) Model and cell membrane partitioning of perfluorooctanesulfonate is independent of the lipid chain length. Colloids Surf B Biointerfaces 76:128–136

    Article  CAS  Google Scholar 

  24. Yeung LWY, Guruge KS, Yamanaka N, Miyakazi S, Lam PKS (2007) Differential expression of chicken hepatic genes responsive to PFOA and PFOS. Toxicology 237:111–125

    Article  CAS  Google Scholar 

  25. Zimmer K, Gutleb AC, Lyche JL, Dahl E, Oskam I, Krogenæs A, Skaare JU, Ropstad E (2008) Altered stress induced cortisol levels in goats exposed to polychlorinated biphenyls (PCB 126 and PCB 153) during foetal and postnatal development. J Toxicol Environ Health A 272:164–172

    Google Scholar 

Download references

Acknowledgments

The studies providing samples for this report received partially funding from ZON MW (JF, TM). Parts of this study performed in Oslo were supported by grant 127534/720 from the Research Council of Norway (RCN). Irene B. Sørvik and Marte B. Tanum are thanked for their assistance with the exposure of the H295R cells. Thanks to Esther Lentzen and Patrick Grysan for producing the NanoSIMS50 images for this publication.

Author information

Authors and Affiliations

  1. Department Environment and Agro-biotechnologies (EVA), Centre de Recherche Public—Gabriel Lippmann, 41, rue de Brill, 4422, Belvaux, Luxembourg

    Arno C. Gutleb, Thomas Udelhoven & Lucien Hoffmann

  2. Toxicology Section, Wageningen University, Tuinlaan 5, 6703 HE, Wageningen, The Netherlands

    Jaime Freitas & Albertinka J. Murk

  3. Wageningen IMARES, P.O. Box 68, 1970 AB, IJmuiden, The Netherlands

    Albertinka J. Murk

  4. Norwegian School of Veterinary Science, 0033, Oslo, Norway

    Steven Verhaegen & Erik Ropstad

  5. Remote Sensing and Geoinformatics Department, Trier University, 54286, Trier, Germany

    Thomas Udelhoven

  6. Département Science et Analyse des Matériaux (SAM), Centre de Recherche Public—Gabriel Lippmann, 41, rue de Brill, 4422, Belvaux, Luxembourg

    Jean-Nicolas Audinot

Authors
  1. Arno C. Gutleb
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jaime Freitas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Albertinka J. Murk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Steven Verhaegen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Erik Ropstad
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Thomas Udelhoven
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lucien Hoffmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jean-Nicolas Audinot
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arno C. Gutleb.

Additional information

Published in the topical collection Emerging Contaminants in Biota with guest editors Yolanda Picó and Damià Barceló.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 156 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gutleb, A.C., Freitas, J., Murk, A.J. et al. NanoSIMS50 — a powerful tool to elucidate cellular localization of halogenated organic compounds. Anal Bioanal Chem 404, 2693–2698 (2012). https://doi.org/10.1007/s00216-012-6066-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-012-6066-8

Keywords

Search

Navigation