Abstract
Arsenic being a toxic metalloid ubiquitously persists in environment and causes several health complications including female reproductive anomalies. Epidemiological studies documented birth anomalies due to arsenic exposure. Augmented reactive oxygen species (ROS) generation and quenched antioxidant pool are foremost consequences of arsenic threat. On the contrary, Vitamin E (VE) and C (VC) are persuasive antioxidants and conventionally used in toxicity management. Present study was designed to explore the extent of efficacy of combined VE and VC (VEC) against Sodium arsenite (NaAsO2) mediated ovarian damage. Thirty-six female Wistar rats were randomly divided into three groups (Grs) and treated for consecutive 30 days; Gr I (control) was vehicle fed, Gr II (treated) was gavaged with NaAsO2 (3 mg/kg/day), Gr III (supplement) was provided with VE (400 mg/kg/day) & VC (200 mg/kg/day) along with NaAsO2. Marked histological alterations were evidenced by disorganization in oocyte, granulosa cells and zona pellucida layers in treated group. Considerable reduction of different growing follicles along with increased atretic follicles was noted in treated group. Altered activities ofΔ5 3β-Hydroxysteroid dehydrogenase and 17β-Hydroxysteroid dehydrogenase accompanied by reduced luteinizing hormone, follicle-stimulating hormone and estradiol levels were observed in treated animals. Irregular estrous cyclicity pattern was also observed due to NaAsO2 threat. Surplus ROS production affected ovarian antioxidant strata as evidenced by altered oxidative stress markers. Provoked oxidative strain further affects DNA status of ovary. However, supplementation with VEC caused notable restoration from such disparaging effects of NaAsO2 toxicities. Antioxidant and antiapoptotic attributes of those vitamins might be liable for such restoration.
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The authors would like to acknowledge Dr. Souryadeep Mukherjee, Department of Life Sciences, Presidency University and Dr. Sumit Mandal, Department of Life Sciences, Presidency University for their help.
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This work was supported by the grant received from Department of Higher Education, Science & Technology and Biotechnology, Government of West Bengal (No. 265(Sanc.) / ST/ P/ S&T/1G-44/2017 dated 25.03.2018). Authors also would like to acknowledge the DST-FIST and DBT BUILDERS grant of the Government of India and FRPDF grant of Presidency University.
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The authors declare that all data were generated in-house and that no paper mill was used. All authors contributed to the study design and experiments. Conceived and designed the experiments: RM, AM, and PKM. Performed the experiments: RM, PP and SB. Analysed the data: RM, PP, SB, AC, AB, AM, and PKM. Wrote the paper: RM. Overall manuscript checked: RM, PP, SB, AC, AB, AM, and PKM. Manuscript critically checked: RM, PP, PKM. Final approval was given by all the authors.
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Mondal, R., Pal, P., Biswas, S. et al. Attenuation of sodium arsenite mediated ovarian DNA damage, follicular atresia, and oxidative injury by combined application of vitamin E and C in post pubertal Wistar rats. Naunyn-Schmiedeberg's Arch Pharmacol 396, 2701–2720 (2023). https://doi.org/10.1007/s00210-023-02491-9
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DOI: https://doi.org/10.1007/s00210-023-02491-9