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Safety evaluation and anti-inflammatory activity of Lactobacillus johnsonii IDCC 9203 isolated from feces of breast-fed infants

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Abstract

This study evaluated the safety of Lactobacillus johnsonii IDCC 9203 and investigated its anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Genomic analysis revealed that this strain has no virulence and antibiotic resistance gene except tetW, which is a tetracycline resistance gene. Minimum inhibitory concentration data showed that the strain is resistant to tetracycline and aminoglycosides. Further analysis indicated that the transferability of the tetW gene is extremely low, and resistance to aminoglycosides is due to the intrinsic resistance of L. johnsonii IDCC 9203. Phenotypic safety assessment showed that the strain has neither β-hemolytic nor β-glucuronidase activity, and no biogenic amine production. When LPS-induced RAW 264.7 cells were treated with L. johnsonii IDCC 9203, the level of nitric oxide and expression of pro-inflammatory cytokines significantly decreased (p < 0.05). Therefore, L. johnsonii IDCC 9203 strain is considered as safe and beneficial probiotic for human consumption.

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Data availability and materials

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Research Foundation of Korea grant funded by the Korean government (Ministry of Science and ICT, MSIT; grant no. 2020R1C1C1005251), and Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through High Value-added Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321033–3).

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Author notes

  1. Olajide Emmanuel Adedeji and Seung A. Chae contributed equally to this study.

Authors and Affiliations

  1. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea

    Olajide Emmanuel Adedeji, O-Hyun Ban, Hyeon Ji Jeon & Young Hoon Jung

  2. Department of Food Science and Technology, Federal University Wukari, P.M.B. 1020, Wukari, Nigeria

    Olajide Emmanuel Adedeji

  3. Ildong Bioscience, Pyeongtaek-si, Gyeonggi-do, 17957, Republic of Korea

    Seung A. Chae, O-Hyun Ban, Won Yeong Bang, Hayoung Kim & Jungwoo Yang

  4. Department of Food Science and Technology, Federal University of Technology Minna, P.M.B 65, Minna, Nigeria

    Chiemela Enyinnaya Chinma

  5. Department of Biotechnology and Food Technology, University of Johannesburg, P.O. Box 17011, Doornfontein Campus, Gauteng, South Africa

    Chiemela Enyinnaya Chinma

Authors
  1. Olajide Emmanuel Adedeji
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  2. Seung A. Chae
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  4. Won Yeong Bang
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  6. Hyeon Ji Jeon
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  7. Chiemela Enyinnaya Chinma
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  8. Jungwoo Yang
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Contributions

OEA conducted statistical analysis, interpreted the data, and wrote the draft manuscript. SAC performed experiment, conducted statistical analysis, and interpreted the data. OB, WYB, HK, and HJJ performed part of the experiment. CEC reviewed and revised the draft manuscript. JY and YHJ conceived the idea, acquired fund, supervised the project, reviewed, and revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Jungwoo Yang or Young Hoon Jung.

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Competing interests

The authors declare no conflict of interest.

Ethical approval

Animal studies were conducted at the Korea Testing and Research Institute (KTRI; Hwasun-gun, Jeollanam-do, Korea) under the Animal Protection Act (no. 14651) and Laboratory Animal Act (no. 15278) of the Korean government.

Additional information

Communicated by Erko Stackebrandt.

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Adedeji, O.E., Chae, S.A., Ban, OH. et al. Safety evaluation and anti-inflammatory activity of Lactobacillus johnsonii IDCC 9203 isolated from feces of breast-fed infants. Arch Microbiol 204, 470 (2022). https://doi.org/10.1007/s00203-022-03097-0

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  • DOI: https://doi.org/10.1007/s00203-022-03097-0

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