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Diversity of soil nitrogen-fixing bacteria in the rhizosphere and non-rhizophere soils of Ebinur Lake Wetland

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Abstract

Ebinur Lake Wetland is an understudied desert wetland ecosystem, particularly regarding nitrogen cycling. This study aimed to ascertain the diversity and richness of nitrogen-fixing bacterial communities in the Ebinur Lake Wetland. The diversity of the nitrogen-fixing bacteria community of nifH genes from the rhizosphere and non-rhizosphere soils of four plants in different seasons were examined using Illumina HiSeq PE250 high-throughput sequencing technology. The correlation between soil environmental factors and diversity and richness of nitrogen-fixing bacteria was studied using the redundancy analysis (RDA). The results showed that the diversity of nitrogen-fixing bacteria in the rhizosphere soil of the constructive plants was higher than that in the non-rhizosphere soil; also, the diversity in July was higher than that in October and April. Geobacter, Pseudomonas and Bradyrhizobium were the dominant common bacteria in different samples of Ebinur Lake Wetland. The RDA showed that the total nitrogen, available potassium and available phosphoruswere significantly correlated with the diversity and richness of nitrogen-fixing bacteria. The diversity and community structure of nitrogen-fixing bacteria in soil samples also changed over time. The community structures of nitrogen-fixing bacteria in the rhizosphere and non-rhizosphere soils of the four plants were not the same during the same period. The correlation between soil environmental factors and the community structure and abundance of nitrogen-fixing bacteria can provide data basis and theoretical support for the degradation and restoration of Ebinur Lake Wetland.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China [Grant number 31560040]. We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work.

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Authors and Affiliations

  1. School of Life Sciences, Shihezi University, Shihezi, 832000, **njiang, People’s Republic of China

    Xue Zhang, Wenge Hu, **tong **, Ting Chen & Yanhui Niu

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  1. Xue Zhang
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  2. Wenge Hu
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  3. **tong **
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Correspondence to Wenge Hu.

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Communicated by Erko Stackebrandt.

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Zhang, X., Hu, W., **, X. et al. Diversity of soil nitrogen-fixing bacteria in the rhizosphere and non-rhizophere soils of Ebinur Lake Wetland. Arch Microbiol 203, 3919–3932 (2021). https://doi.org/10.1007/s00203-021-02363-x

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  • DOI: https://doi.org/10.1007/s00203-021-02363-x

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