SpringerLink
Log in

Rhodohalobacter mucosus sp. nov., isolated from a marine solar saltern

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

A Gram-stain-negative, non-motile, aerobic, rod-shaped and reddish-pigmented bacterium, designated 8A47T, was isolated from a marine solar saltern located in Wendeng, PR China. The novel strain 8A47T grows at 20–42 °C, pH 7.0–9.0, and in the presence of 2.0–14.0% (w/v) NaCl. Optimal growth was observed at 37–40 °C, pH 7.5–8.0, and with 4.0–6.0% (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 8A47T formed an evolutionary lineage with members of the genus Rhodohalobacter. Strain 8A47T exhibited high level of similarity to Rhodohalobacter barlenses MCCC 1K03442T (94.7%) and Rhodohalobacter halophilus JZ3C29T (93.5%). The major fatty acids were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0 and iso-C15:0. The sole respiratory quinone was MK-7. The polar lipids of the new isolate consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid, two unidentified phospholipids and three unidentified glycolipids and. The genomic DNA G + C content of the strain 8A47T was 47.7 mol%. Based on its phenotypic, chemotaxonomic, genotypic and genomic characteristics presented in this study, strain 8A47T is considered to represent a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter mucosus sp. nov. is proposed. The type strain is 8A47T (= KCTC 62603T = MCCC 1H00329T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868

    Article  CAS  Google Scholar 

  • CLSI (2012) Performance standards for antimicrobial susceptibility testing, twenty-second informational supplement CLSI document M100–S22. Clinical and Laboratory Standards Institute, Wayne

    Google Scholar 

  • Cowan ST, Steel KJ (1974) Bacterial characters and characterization, 2nd edn. Cambridge University Press, Cambridge

    Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  CAS  Google Scholar 

  • Fitch W (1971) Towards de ning course of evolution: minimum change for a specified tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P, Tiedje JM (2007) DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57:81–91

    Article  CAS  Google Scholar 

  • Han SB, Yu YH, Ju Z, Li Y, Zhang R, Hou XJ, Ma XY, Yu XY, Sun C, Wu M (2018) Rhodohalobacter barkolensis sp. nov., isolated from a saline lake and emended description of the genus Rhodohalobacter. Int J Syst Evol Microbiol 68:1949–1954

    Article  CAS  Google Scholar 

  • Kates M, Work TS (1972) Techniques of lipidology: isolation, analysis, and identification of lipids. Lab Tech Biochem Mol Biol 3:0151–0155

    Google Scholar 

  • Kim OS, Cho Y-J, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  Google Scholar 

  • Kimura M (1981) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  Google Scholar 

  • Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Lee I, Kim YO, Park SC (2015) OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 66:1100–1103

    Article  Google Scholar 

  • Liu QQ, Li XL, Rooney AP, Du ZJ, Chen GJ (2014) Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae. Int J Syst Evol Microbiol 64:3473–3477

    Article  CAS  Google Scholar 

  • Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60

    Article  Google Scholar 

  • Mu DS, Liang QY, Wang XM, Lu DC, Shi MJ, Chen GJ, Du ZJ (2018) Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 6:230

    Article  Google Scholar 

  • Rodriguez-R LM, Konstantinidis KT (2014) Bypassing cultivation to identify bacterial species. Microbe 9:111–118

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Sasser M (1990) Identification of Bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. Microbial ID Inc, Newark

    Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society For Microbiology, Washington, pp 611–651

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  • Tindall BJ, Sikorski J, Smibert RA, Krieg NR (2007) Phenotypic Characterization and the Principles of Comparative Systematics. Methods for General and Molecular Microbiology, Third Edition. American Society for Microbiology, Washington, DC, pp 330–393

  • **a J, **e ZH, Dunlap CA, Rooney AP, Du ZJ (2017) Rhodohalobacter halophilus gen. nov., sp. nov., a moderately halophilic member of the family Balneolaceae. Int J Syst Evol Microbiol 67:1281–1287

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work of scanning electron microscope was supported by the Physical-Chemical Materials Analytical & Testing Center of Shandong University at Weihai.

Funding

This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).

Author information

Authors and Affiliations

  1. Marine College, Shandong University, Weihai, 264209, Shandong, People’s Republic of China

    Tao-Jie Wang, Zhen-Wei Liu, ** Feng, Qi-Hang Zou & Zong-Jun Du

  2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, People’s Republic of China

    Zong-Jun Du

Authors
  1. Tao-Jie Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhen-Wei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. ** Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qi-Hang Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zong-Jun Du
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zong-Jun Du.

Ethics declarations

Conflict of interest

Authors declare that there are no conflicts of interest. The article does not contain any studies with no conflicts of interest.

Additional information

Communicated by Erko Stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The GenBank accession numbers for the 16S rRNA gene sequence and the draft whole genome data of strain 8A47T are MG696673 and QGGB00000000, respectively.

Supplementary Information

Below is the link to the electronic supplementary material.

203_2021_2254_MOESM1_ESM.pdf

Two supplementary figures and one table with the online version of this paper: The figure of micrograph observed by scanning electron microscope showing the morphology of cells and the polar lipids figure. The table shows all negative traits of strain 8A47T from API 20E, API ZYM, API 50CHB and Biolog GEN III identification systems. (PDF 256 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, TJ., Liu, ZW., Feng, X. et al. Rhodohalobacter mucosus sp. nov., isolated from a marine solar saltern. Arch Microbiol 203, 2419–2424 (2021). https://doi.org/10.1007/s00203-021-02254-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-021-02254-1

Keywords

Search

Navigation