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Bacillus salipaludis sp. nov., isolated from saline–alkaline soil

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Abstract

A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline–alkali wetland soil of Tian**, China. Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%). However, the average nucleotide identity (ANI) and digital DNA–DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species. The strain grew at 19–42 °C (optimally 33–37 °C) in the presence of 3–20% (w/v) NaCl (optimally 8–12%(w/v)), and at pH 6.5–11.0 (optimally 7.5–8.5). The major cellular fatty acids were iso-C15:0 (24.8%) and anteiso-C15:0 (38.9%). The predominant polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp. nov. is proposed. The type strain is WN066T (= KCTC 33953T = ACCC 60085T).

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Acknowledgements

We would like to thank Prof. Aharon Oren for very valuable help in naming the organism.

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Authors and Affiliations

  1. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tian** University, **nan District, Yaguan Road 135, Tian**, 300350, People’s Republic of China

    Limin Xue & Jianjun Qiao

  2. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Bei**g, 100081, People’s Republic of China

    Liu Tang, Juanjuan Zhao, Zhenjuan Fang, Hanxu Liu & Guishan Zhang

Authors
  1. Limin Xue
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  2. Liu Tang
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  3. Juanjuan Zhao
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  4. Zhenjuan Fang
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  5. Hanxu Liu
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  6. Jianjun Qiao
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  7. Guishan Zhang
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Contributions

LX contributed to performing the experiments and writing the initial draft. LT provided samples of experiment and participated in the isolation and cultivation of strains. GZ and JQ contributed to the guidance of experimental operations. JZ and ZF contributed to the morphological analyses. JZ and HL performed genome analysis. GZ and JQ contributed to reagents, instrumentation and the financial support for this work. All the authors approved the manuscript.

Corresponding authors

Correspondence to Jianjun Qiao or Guishan Zhang.

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Communicated by Erko Stackebrandt.

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Xue, L., Tang, L., Zhao, J. et al. Bacillus salipaludis sp. nov., isolated from saline–alkaline soil. Arch Microbiol 203, 2211–2217 (2021). https://doi.org/10.1007/s00203-021-02239-0

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  • DOI: https://doi.org/10.1007/s00203-021-02239-0

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