Abstract
Key message
QPL_6D.1b displayed an additive effect with Rht-B1b and Rht-D1b in reducing wheat plant height and peduncle length, which confers shorter peduncle length and more kernels per spike, and had been broadly selected by Chinese modern wheat cultivars.
Abstract
Peduncle length (PL), as the key component of wheat plant height (PH), plays critical role in determining wheat lodging resistance and wheat pathogen resistance; then, its breeding selection and genetic basis remain largely unclear. Here the PH and PL were investigated in 406 wheat accessions in eight environments. In this study, a PL preferentially QTL QPL_6D.1 was identified in six environments by GWAS, which explained 13.6–24.2% of wheat PL variations in natural population. The allele QPL_6D.1b displayed a significantly additive effect with Rht-B1b and Rht-D1b in controlling PH and PL and could freely combined with Rht-B1b and Rht-D1b in current wheat cultivars. Haplotypic analysis demonstrates the QPL_6D.1b has been selected by Chinese modern wheat cultivar and confers shorter PL and more kernels per spike, highlighting its potentials in wheat breeding.
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Data availability statement
All data, models or code generated or used during the study is available from the corresponding author by request. The phenotypes and SNP data are available in https://iwheat.net/resource/ for free download.
References
Bazhenov MS, Divashuk MG, Amagai Y, Watanabe N, Karlov GI (2015) Isolation of the dwarfing Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker. Mol Breed 35:1–8
Bridgemohan P, Bridgemohan R (2014) Evaluation of anti-lodging plant growth regulators on the growth and development of rice (Oryza sativa). J Cereals Oilseeds 5(3):12–16
Buss W, Ford BA, Foo E, Schnippenkoetter W, Borrill P, Brooks B, Ashton AR, Chandler PM, Spielmeyer W (2020) Overgrowth mutants determine the causal role of gibberellin GA2oxidaseA13 in Rht12 dwarfism of wheat. J Exp Bot 71:7171–7178
Cao W, Somers DJ, Fedak G (2009) A molecular marker closely linked to the region of Rht-D1c and Ms2 genes in common wheat (Triticum aestivum). Genome 52:95–99
Chai L, **n M, Dong C, Chen Z, Zhai H, Zhuang J, Cheng X, Wang N, Geng J, Wang X, Bian R, Yao Y, Guo W, Hu Z, Peng H, Bai G, Sun Q, Su Z, Liu J, Ni Z (2022) A natural variation in Ribonuclease H-like gene underlies Rht8 to confer “Green Revolution” trait in wheat. Mol Plant 15:377–380
Chen S, Gao R, Wang H, Wen M, **ao J, Bian N, Zhang R, Hu W, Cheng S, Bie T, Wang X (2014) Characterization of a novel reduced height gene (Rht23) regulating panicle morphology and plant architecture in bread wheat. Euphytica 203:583–594
Dixon LE, Pasquariello M, Boden SA (2020) TEOSINTE BRANCHED1 regulates height and stem internode length in bread wheat. J Exp Bot 71:4742–4750
Ford BA, Foo E, Sharwood R, Karafiatova M, Vrana J, MacMillan C, Nichols DS, Steuernagel B, Uauy C, Dolezel J, Chandler PM, Spielmeyer W (2018) Rht18 Semidwarfism in wheat is due to increased GA 2-oxidaseA9 expression and reduced GA content. Plant Physiol 177:168–180
Hedden P (2003) The genes of the Green Revolution. Trends Genet TIG 19:5–9
Hirsch CN, Flint-Garcia SA, Beissinger TM, Eichten SR, Deshpande S, Barry K, McMullen MD, Holland JB, Buckler ES, Springer N, Buell CR, de Leon N, Kaeppler SM (2014) Insights into the effects of long-term artificial selection on seed size in maize. Genetics 198:409–421
Li A, Yang W, Guo X, Liu D, Sun J, Zhang A (2012) Isolation of a gibberellin-insensitive dwarfing gene, Rht-B1e, and development of an allele-specific PCR marker. Mol Breed 30:1443–1451
Li R, Zeng Y, Xu J, Wang Q, Wu F, Cao M, Lan H, Liu Y, Lu Y (2015) Genetic variation for maize root architecture in response to drought stress at the seedling stage. Breed Sci 65:298–307
Li F, Wen W, Liu J, Zhang Y, Cao S, He Z, Rasheed A, ** H, Zhang C, Yan J, Zhang P, Wan Y, **a X (2019) Genetic architecture of grain yield in bread wheat based on genome-wide association studies. BMC Plant Biol 19:168
Li C, Tang H, Luo W, Zhang X, Mu Y, Deng M, Liu Y, Jiang Q, Chen G, Wang J, Qi P, Pu Z, Jiang Y, Wei Y, Zheng Y, Lan X, Ma J (2020) A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat. Theor Appl Genet 133:3381–3393
Liu X, Huang M, Fan B, Buckler ES, Zhang Z (2016) Iterative usage of fixed and random effect models for powerful and efficient genome-wide association studies. PLoS Genet 12:e1005767
Liu Z, Hu Z, Lai X, Cao J, Zhang J, Ma X, Zhang X, Wang X, Ji W, Xu S (2022) Multi-environmental population phenoty** suggest the higher risks of wheat Rht-B1b and Rht-D1b cultivars in global warming scenarios. bioRxiv
Lu Y, **ng L, **ng S, Hu P, Cui C, Zhang M, **ao J, Wang H, Zhang R, Wang X, Chen P, Cao A (2015) Characterization of a Putative New Semi-Dominant Reduced Height Gene, Rht_NM9, in Wheat (Triticum aestivum L.). J Genet Genomics 42:685–698
Lujan Basile SM, Ramirez IA, Crescente JM, Conde MB, Demichelis M, Abbate P, Rogers WJ, Pontaroli AC, Helguera M, Vanzetti LS (2019) Haplotype block analysis of an Argentinean hexaploid wheat collection and GWAS for yield components and adaptation. BMC Plant Biol 19:553
Ma J, Ding P, Liu J, Li T, Zou Y, Habib A, Mu Y, Tang H, Jiang Q, Liu Y (2019) Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat. Theor Appl Genet 132:3155–3167
Masubelele NH, Dewitte W, Menges M, Maughan S, Collins C, Huntley R, Nieuwland J, Scofield S, Murray JA (2005) D-type cyclins activate division in the root apex to promote seed germination in Arabidopsis. Proc Natl Acad Sci USA 102:15694–15699
Menges M, Samland AK, Planchais S, Murray JA (2006) The D-type cyclin CYCD3;1 is limiting for the G1-to-S-phase transition in Arabidopsis. Plant Cell 18:893–906
Ohyama A, Tominaga R, Toriba T, Tanaka W (2022) D-type cyclin OsCYCD3;1 is involved in the maintenance of meristem activity to regulate branch formation in rice. J Plant Physiol 270:153634
Pearce S, Saville R, Vaughan SP, Chandler PM, Wilhelm EP, Sparks CA, Al-Kaff N, Korolev A, Boulton MI, Phillips AL, Hedden P, Nicholson P, Thomas SG (2011) Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat. Plant Physiol 157:1820–1831
Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Sudhakar D, Christou P, Snape JW, Gale MD, Harberd NP (1999) “Green revolution” genes encode mutant gibberellin response modulators. Nature 400:256–261
Peng ZS, Li X, Yang ZJ, Liao ML (2011) A new reduced height gene found in the tetraploid semi-dwarf wheat landrace Aiganfanmai. Genet Mol Res 10:2349–2357
Piepho H, Möhring J, Melchinger A, Büchse A (2008) BLUP for phenotypic selection in plant breeding and variety testing. Euphytica 161:209–228
Puran B, Ronell SB (2014) Evaluation of anti-lodging plant growth regulators on the growth and development of rice (Oryza sativa). J Cereals Oilseeds 5:12–16
Reynolds M, Foulkes MJ, Slafer GA, Berry P, Parry MA, Snape JW, Angus WJ (2009) Raising yield potential in wheat. J Exp Bot 60:1899–1918
Sun L, Yang W, Li Y, Shan Q, Ye X, Wang D, Yu K, Lu W, **n P, Pei Z, Guo X, Liu D, Sun J, Zhan K, Chu J, Zhang A (2019) A wheat dominant dwarfing line with Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line. Plant J 97:887–900
Tester M, Langridge P (2010) Breeding technologies to increase crop production in a changing world. Science 327:818–822
Tian X, **a X, Xu D, Liu Y, **e L, Hassan MA, Song J, Li F, Wang D, Zhang Y, Hao Y, Li G, Chu C, He Z, Cao S (2022) Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat. New Phytol 233:738–750
Wang X, Zhao P, Guo X, Liu Z, Ma X, Zhao Y, Lai X, Huang L, Wang W, Han D (2022) Population sequencing reveals Rht-D1b contributing the bigger seedling root to modern wheat cultivars. bioRxiv
Weir BS, Cockerham C (1996) Genetic data analysis II: methods for discrete population genetic data. Sinauer Assoc. Inc, Sunderland
Wu J, Yu R, Wang H, Zhou C, Huang S, Jiao H, Yu S, Nie X, Wang Q, Liu S, Weining S, Singh RP, Bhavani S, Kang Z, Han D, Zeng Q (2021) A large-scale genomic association analysis identifies the candidate causal genes conferring stripe rust resistance under multiple field environments. Plant Biotechnol J 19:177–191
Wurschum T, Langer SM, Longin CFH, Tucker MR, Leiser WL (2017) A modern green revolution gene for reduced height in wheat. Plant J 92:892–903
**ong H, Zhou C, Fu M, Guo H, **e Y, Zhao L, Gu J, Zhao S, Ding Y, Li Y, Zhang J, Wang K, Li X, Liu L (2022) Cloning and functional characterization of Rht8, a “Green Revolution” replacement gene in wheat. Mol Plant 15:373–376
Yu M, Mao S-L, Chen G-Y, Pu Z-E, Wei Y-M, Zheng Y-L (2014) QTLs for uppermost internode and spike length in two wheat RIL populations and their affect upon plant height at an individual QTL level. Euphytica 200:95–108
Zhang Y, Zhu Y, Peng Y, Yan D, Li Q, Wang J, Wang L, He Z (2008) Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice. Cell Res 18:412–421
Zhang N, Fan X, Cui F, Zhao C, Zhang W, Zhao X, Yang L, Pan R, Chen M, Han J, Ji J, Liu D, Zhao Z, Tong Y, Zhang A, Wang T, Li J (2017) Characterization of the temporal and spatial expression of wheat (Triticum aestivum L.) plant height at the QTL level and their influence on yield-related traits. Theor Appl Genet 130:1235–1252
Zhou Y, Zhao X, Li Y, Xu J, Bi A, Kang L, Xu D, Chen H, Wang Y, Wang YG, Liu S, Jiao C, Lu H, Wang J, Yin C, Jiao Y, Lu F (2020) Triticum population sequencing provides insights into wheat adaptation. Nat Genet 52:1412–1422
Zhu Y, Nomura T, Xu Y, Zhang Y, Peng Y, Mao B, Hanada A, Zhou H, Wang R, Li P, Zhu X, Mander LN, Kamiya Y, Yamaguchi S, He Z (2006) ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Plant Cell 18:442–456
Acknowledgements
We thank Pro. Guoyue Chen and Pro. Zhien Pu for field investigations in Sichuan Province.
Funding
This study was funded by the “Integration of Two Chains” Key Research and Development Projects of Shaanxi Province “Wheat Seed Industry Innovation Project” and National Natural Science Foundation of China [31571756 and 31501380] and Key R&D Program of Yangling Seed Industry Innovation Center (Ylzy-xm-01).
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SB Xu and WQ Ji designed the concept and experiments. ZH Liu and XJ Lai performed field investigation. ZH Liu, P Zhao, XM Wang and SB Xu analyzed the data and wrote the manuscript. All the authors were involved in the revision of the manuscript and approved the final manuscript.
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Figure S1
Manhattan and Q–Q plots for plant height and peduncle length. The dash line indicates the P = 1 × 10−4. The black line indicates the P =1 × 10−7. The data come from the BLUP values (n = 406) of eight conditions, as shown in Table S1. (TIF 12538 kb)
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Liu, Z., Zhao, P., Lai, X. et al. The selection and application of peduncle length QTL QPL_6D.1 in modern wheat (Triticum aestivum L.) breeding. Theor Appl Genet 136, 32 (2023). https://doi.org/10.1007/s00122-023-04274-5
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DOI: https://doi.org/10.1007/s00122-023-04274-5