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Pm61: a recessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis

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A single recessive powdery mildew resistance gene Pm61 from wheat landrace Xuxusanyuehuang was mapped within a 0.46-cM genetic interval spanning a 1.3-Mb interval of the genomic region of chromosome arm 4AL.

Abstract

Epidemics of powdery mildew incited by the biotrophic fungus Blumeria graminis f. sp. tritici (Bgt) have caused significant yield reductions in many wheat (Triticum aestivum)-producing regions. Identification of powdery mildew resistance genes is required for sustainable improvement of wheat for disease resistance. Chinese wheat landrace Xuxusanyuehuang was resistant to several Bgt isolates at the seedling stage. Genetic analysis based on the inoculation of Bgt isolate E09 on the F1, F2, and F2:3 populations produced by crossing Xuxusanyuehuang to susceptible cultivar Mingxian 169 revealed that the resistance of Xuxusanyuehuang was controlled by a single recessive gene. Bulked segregant analysis and simple sequence repeat (SSR) map** placed the gene on chromosome bin 4AL-4-0.80-1.00. Comparative genomics analysis was performed to detect the collinear genomic regions of Brachypodium distachyon, rice, sorghum, Aegilops tauschii, T. urartu, and T. turgidum ssp. dicoccoides. Based on the use of 454 contig sequences and the International Wheat Genome Sequence Consortium survey sequence of Chinese Spring wheat, four EST-SSR and seven SSR markers were linked to the gene. An F5 recombinant inbred line population derived from Xuxusanyuehuang × Mingxian 169 cross was used to develop the genetic linkage map. The gene was localized in a 0.46-cM genetic interval between Xgwm160 and **csx79 corresponding to 1.3-Mb interval of the genomic region in wheat genome. This is a new locus for powdery mildew resistance on chromosome arm 4AL and is designated Pm61.

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Acknowledgments

The authors are grateful to Dr. R. L. Conner of Morden Research and Developmental Center, Agriculture and Agri-Food Canada for his critical review of the manuscript, Drs. W. J. Raupp and B. S. Gill of Wheat Genetics Resource Centre, Kansas State University, USA, for providing the Chinese Spring aneuploid and deletion lines, and J Zou for technical assistance. Financial support of this research by the National Natural Science Foundation of China (31501310 and 31471491), the National Key Research and Development Program of China (2017YFD0101000), Scientific and Technological Research Project of Henan Province of China (172102110110), the CAAS Innovation Team and the National Engineering Laboratory of Crop Molecular Breeding, Henan Province Young College Key Teacher Subsidy Program (2017GGJS177) are gratefully appreciated.

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Author notes

  1. Huigai Sun and **ghuang Hu have contributed equally to this work.

Authors and Affiliations

  1. The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Bei**g, 100081, China

    Huigai Sun, **ghuang Hu, Wei Song, Dan Qiu, Peipei Wu, Hongjun Zhang, Hongwei Liu, Li Yang, Yunfeng Qu, Yahui Li, Teng Li, Yang Zhou & Hongjie Li

  2. College of Chemistry and Environment Engineering, **dingshan University, **dingshan, 467000, China

    Wei Cheng & **gting Li

  3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Bei**g, 100101, China

    Zhiyong Liu

  4. College of Life and Environmental Science, Minzu University of China, Bei**g, 100081, China

    Huigai Sun

  5. College of Life Science and Technology, Harbin Normal University, Harbin, 150080, China

    Yunfeng Qu

  6. College of Biology, Hunan University, Changsha, 410082, China

    Wei Song

  7. Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, China

    Lei Cui

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Corresponding authors

Correspondence to Zhiyong Liu, **gting Li or Hongjie Li.

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Additional information

Communicated by Steven S. Xu.

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Sun, H., Hu, J., Song, W. et al. Pm61: a recessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis. Theor Appl Genet 131, 2085–2097 (2018). https://doi.org/10.1007/s00122-018-3135-1

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