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A novel major quantitative trait locus controlling seed development at low temperature in soybean (Glycine max)

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Abstract

Low temperature is among the critical environmental factors that limit soybean production. To elucidate the genetic basis for chilling tolerance and identify useful markers, we conducted quantitative trait loci (QTL) analysis of seed-yielding ability at low temperature in soybean (Glycine max), using artificial climatic environments at usual and low temperatures and recombinant inbred lines derived from a cross between two contrasting cultivars in terms of chilling tolerance. We identified a QTL of a large effect (LOD > 15, r 2 > 0.3) associated with seed-yielding ability only at low temperature. The QTL was mapped near marker Sat_162 on linkage group A2, where no QTL for chilling tolerance has previously been identified. The tolerant genotype did not increase the pod number but maintained the seed number per pod and single seed weight, namely, the efficiency of seed development at low temperature. The effect of the QTL was confirmed in a segregating population of heterogeneous inbred families, which provided near-isogenic lines. The genomic region containing the QTL also influenced the node and pod numbers regardless of temperature condition, although this effect was not primarily associated with chilling tolerance. These results suggest the presence of a new major genetic factor that controls seed development specifically at low temperature. The findings will be useful for marker-assisted selection as well as for understanding of the mechanism underlying chilling tolerance in reproductive organs.

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Acknowledgments

The authors thank Dr. Y. Sato for his encouragement and support for this work. The technical assistance of R. Narita, S. Furuhata, K. Yoshida and R. Sugisawa is gratefully acknowledged. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 18580011) and by a grant from the National Agricultural Research Organization (Development of Innovative Crops through the Molecular Analysis of Useful Genes) to H. F.

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  1. Shizen Ohnishi

    Present address: Hokkaido Central Agricultural Experiment Station, Naganuma, Hokkaido, 069-1395, Japan

Authors and Affiliations

  1. Department of Plant Genetic Resources, Graduate School of Agriculture, Hokkaido University, 9 Kita 9 Nishi, Sapporo, Hokkaido, 060-8589, Japan

    Tatsuya Ikeda & Keisuke Kitamura

  2. Tokachi Agricultural Experiment Station, Shinnsei, Memuro, Hokkaido, 082-0071, Japan

    Shizen Ohnishi & Tomoaki Miyoshi

  3. Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori, 036-8561, Japan

    Mineo Senda

  4. National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Toyohira-Ku, Sapporo, Hokkaido, 062-8555, Japan

    Masao Ishimoto & Hideyuki Funatsuki

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  1. Tatsuya Ikeda
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Correspondence to Hideyuki Funatsuki.

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Communicated by H. T. Nguyen.

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Ikeda, T., Ohnishi, S., Senda, M. et al. A novel major quantitative trait locus controlling seed development at low temperature in soybean (Glycine max). Theor Appl Genet 118, 1477–1488 (2009). https://doi.org/10.1007/s00122-009-0996-3

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  • DOI: https://doi.org/10.1007/s00122-009-0996-3

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