Zusammenfassung
Mit Hilfe einer neuen Technik wird eine azelluläre Matrix aus einem porcinen Dünndarmsegment unter Erhaltung des mesenterialen Arterien- und Venenstamms gewonnen. Eine erneute Besiedelung dieser biologischen vaskularisierten Matrix (BioVaM) mit funktionellen Zellen, d. h. primären glatten Muskel- und Urothelzellen, sowie des Gefäßbetts mit endothelialen Vorläuferzellen resultiert in einem mittels Tissue Engineering gewonnenen und durchbluteten Gewebe für die Rekonstruktion und Augmentation der Harnblase. Erste erfolgreiche Kurzzeitimplantationen dieser Matrix im Schweinemodell zur Beurteilung der frühen Implantatperfusion nach Gefäßanastomose auf den Empfängerorganismus werden vorgestellt.
Abstract
A new technique is presented to harvest an acellular matrix from a porcine small bowel segment preserving the mesenteric arterial and venous pedicles. Reseeding of this biological vascularized matrix (BioVaM) with functional cells, i.e. smooth muscle and urothelial cells isolated from the urinary tract, and resurfacing of its vascular structures with endothelial precursor cells results in a vascularized tissue engineered graft for reconstruction and augmentation of the urinary bladder. First promising short term implantation experiments using a porcine model for the evaluation of early graft perfusion after vascular anastomosis are presented.
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Schultheiss, D., Gabouev, A.I., Kaufmann, P.M. et al. Biologische vaskularisierte Matrix (BioVaM). Urologe [A] 43, 1223–1228 (2004). https://doi.org/10.1007/s00120-004-0702-7
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DOI: https://doi.org/10.1007/s00120-004-0702-7