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Decrease in toll-like receptors 2 and 4 in the spleen of mouse with endotoxic tolerance

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Abstract.

Objective:

Down-regulation of toll-like receptor (TLRs) is present in animals with endotoxin tolerance. The spleen is the largest peripheral lymphatic organ in vivo, and it plays an important role in inflammatory reactivity. In this study we investigated TLR2 and TLR4 proteins in the spleen of endotoxic tolerant mice.

Methods:

The TLR2 and TLR4 proteins were quantitatively detected by Western blotting, and their locations in the spleen were observed by immunohistochemistry. IL1-β mRNA in the spleen was analyzed by real-time quantitative PCR.

Results:

The mice pretreated with 0.5 mg/kg/day of LPS for 14 days exhibited a decrease of TLR2 and TLR4, and presented endotoxin tolerance. In addition, a bolus injection of 10 mg/kg LPS for mice with endotoxic tolerance caused no alteration of TLR2 and TLR4 proteins and no change of IL1-β mRNA expression in the spleen.

Conclusion:

TLR4 is the target of endotoxic tolerance induction. The induction of cross-tolerance of TLR2 following LPS challenge is present in vivo with endotoxin tolerance.

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Authors and Affiliations

  1. Department of Pathogenic Biology, School of Medicine, Suzhou University, 199 Reng-al Roud, Dushu Lake Higher Eduction Town, Suzhou, Jiangsu, 215123, PR China

    B. Zhong & C. Ming **a

  2. The Second Hospital of Nan**g, affiliated with Medical School, Southeast University, 1-1 Zhong-fu Road, Nan**g, Jiangsu, 210003, PR China

    B. Zhong, H. Ying Ma, Q. Yang & G. Qing Yin

  3. Nan**g Agricultural University, 6 Tong-wei Road, Nan**g, Jiangsu, 210095, PR China

    F. Rong Gu

Authors
  1. B. Zhong
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  2. H. Ying Ma
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  3. Q. Yang
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  4. F. Rong Gu
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  5. G. Qing Yin
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  6. C. Ming **a
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Corresponding authors

Correspondence to G. Qing Yin or C. Ming **a.

Additional information

Received 21 May 2007; returned for revision 4 June 2007; returned for final revision 1 October 2007; accepted by V. Kumar 8 December 2007

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Zhong, B., Ma, H.Y., Yang, Q. et al. Decrease in toll-like receptors 2 and 4 in the spleen of mouse with endotoxic tolerance. Inflamm. res. 57, 252–259 (2008). https://doi.org/10.1007/s00011-007-7104-4

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  • DOI: https://doi.org/10.1007/s00011-007-7104-4

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