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Development and validation of a sensitive LC-MS method for the determination of Promethazine hydrochloride in human plasma and urine

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Summary

We developed and validated a sensitive and low sample volume liquid chromatographic-mass spectrometric (LC-MS) method for determination of Promethazine hydrochloride in human plasma (0.5 ml) or urine (0.1 ml). The lower limit of quantification in human plasma and urine was 1.00 ng/ml. The inter- and intra-day precisions (CV %) in both plasma and urine were lower than 10%, the mean method accuracies and recoveries from spiked plasma samples at three concentrations were more than 97%. The developed method was successfully applied to determine Promethazine hydrochloride in human plasma and urine, and proved suitable to clinical pharmacokinetic study.

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Authors and Affiliations

  1. Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, 250012, **an, China

    ** Liu, Ben-Jie Wang & Rui-Chen Guo

  2. Department of Cardiac Surgery, The Second Affiliated Hospital of Shandong Traditional Chinese Medicine University, 250001, **an, China

    Sun Liang

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  1. ** Liu
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  2. Sun Liang
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  3. Ben-Jie Wang
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  4. Rui-Chen Guo
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Correspondence to Rui-Chen Guo.

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Liu, P., Liang, S., Wang, BJ. et al. Development and validation of a sensitive LC-MS method for the determination of Promethazine hydrochloride in human plasma and urine. Eur. J. Drug Metabol. Pharmacokinet. 34, 177–184 (2009). https://doi.org/10.1007/BF03191171

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  • DOI: https://doi.org/10.1007/BF03191171

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