Abstract
A novel method of estimating conformation of protein secondary structure withinFusarium cells under the influence of growth cultures and bodies was studies by Fourier-Transform Infrared (FT-IR) spectroscopy combined with a deconvolution procedure of amide I band. After infrared data collection and curve-fitting process, the percentages of secondary structure components (a-helix, β-sheet, turn, random coil and β-antiparallel) were obtained. It showed structural diversity among differentFusarium species and variations caused by growth cultures and bodies. The method provides a rapidly quantitative technique to study cell structure of fungi.
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Wan, J., Liu, Y., Lan, L. et al. Conformational study of protein in some species ofFusarium by FT-IR spectroscopy. Ann. Microbiol. 58, 169–171 (2008). https://doi.org/10.1007/BF03179463
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DOI: https://doi.org/10.1007/BF03179463