Abstract
The production of the cytokines, interferon-gamma and tumour necrosis factor by human antral mucosa cells and stimulated peripheral blood mononuclear cells were determined by enzyme linked immunosorbant assay and L929 bioassay respectively. Tumour necrosis factor production by peripheral blood mononuclear cells in response toHelicobacter pylori stimulation was depressed inHelicobacter pylori positive individuals, compared toHelicobacter pylori negative individuals (P < 0.05). There was no difference in tumour necrosis factor production by peripheral blood mononuclear cells in response to purified protein derivative. However, tumour necrosis factor production by cells isolated from gastric mucosa during short term culture was significantly higher inHelicobacter pylori positive patients (P < 0.05) than negative patients, indicating a probable macrophage response. Levels of interferon-gamma did not differ significantly in the gastric explant culture from the two groups. The results show thatHelicobacter pylori negative patients have a stronger peripheral cellular immune response toHelicobacter pylori infection. The higher levels of tumour necrosis factor production by antral mucosa cells inHelicobacter pylori positive patients may reflect the infiltration of T lymphocytes and macrophages within the local mucosa.
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References
Marshall, B.J. Unidentified curved bacilli on gastric epithelium in chronic gastritis. Lancet. 1983; 1: 1273–1275.
Goodwin, C. S., Armstrong, A. and Marshall, B.Campylobacter pylori gastritis and peptic ulcer. J. Clin. Microbiol. 1986; 39: 353–356.
Parsonnet, J., Friedman, G. D., Daniel, M. S., Vandersteen, P., Ghang, Y., Vogelman, J. H., Orentreich, D. E. E. N., Sibley, R. K.Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 1991; 325: 1127–31.
Nomura, A., Stemmermann, G. N., Chyou, P. H., Kato, I., Perez-Perez, G. I., Blaser, M. J.Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N. Engl. J. Med. 1991; 325: 1132–36.
Perez-perez, G. I., Workin, D. B., Chodos, M. J. E., Blaser, M. J.Campylobacter pylori antibodies in humans. Ann. Intern. Med. 1988; 109: 11–17.
Karttunen, R., Anderssod, G., Poikonen, K., Kosunen, T. U., Karttunen, T., Juutinen, K., Niemela, S.Helicobacter pylori lymphocyte activation in peripheral blood cultures. Clin. Exp. Immunol. 1990; 82: 485–488.
Crabtree, J. E., Rathbone, B. J., Heatley, R. V., Shallcross, T. M., Wyatt, J. I., Losowsky, M. S. Duodenal secretion ofCampylobacter pylori specific antibodiesin vitro. In: Megraud, F., Lamouliatte, J., eds. Gastroduodenal pathology andCampylobacter pylori. Amsterdam: Excerpta Medica, 1989; 341–4.
Rathbone, B. J., Wyatt, J. I., Worsley, B. W., Shires, S. E., Trejdosiewicz, L. K., Heatley, R. V., Losowsky, M. S. Systemic and local antibody responses to gastricCampylobacter pyloridis in non-ulcer dyspepsia. Gut 1986; 27: 642–7.
Paull, C. S., Armstrong, J. A., Marshall, B. J.Campylobacter pyloridis, gastric and peptic ulceration. J. Clin. Pathol. 1986; 39: 353–365.
Paull, G., Yardley, J. Pathology ofCampylobacter pylori associated gastric and oesophageal lesions. InCampylobacter pylori in Gastritis and Peptic Ulcer Disease. M. J. Blaser, editor. Igaku-Shoin Medical Publishers, New York. 1989; 73–97.
Brenner, D. A., Buck, M., Feitelberg, S. P., Chojkier, M. Tumor Necrosis Factor-α inhibits albumin gene expression in a marine model of cachexia. J.Clin. Invest. 1990; 85: 248–255.
Tracey, Kevin J. and Helen Vlassara. Peptid Regulatory Factors. Cachectin/Tumour Necrosis Factor. Lancet. 1989; 20: 1122–27.
O’Garra, Anne. Peptide Regulatory Factors. Interleukins and the immune system 2. Lancet. 1989; 6: 1003–1005.
Le, J., Vilcek, J. Tumour necrosis factor and interleukin 1: cytokines with multiple overlap** biological activities. Lab Invest. 1987; 56: 234–8.
Smith, K. A. T-cell growth factor. Immunol. Rev. 1980; 51: 337–357.
Frances, R. Balkwill. Peptide Regulatory Factors. Interferon. Lancet 1989; 13: 1060–1063.
Trinchieri, G., Perussia, B. Immune interferon: a pleiotropic lymphokine with multiple effects. Immunol Today. 1985; 6: 131–36.
Westerberg, H., Gustafson, S. A., Nordenskjold, B., Silversward, C., Wallgren, A. Oestrogen receptor level and other factors in early recurrence of breast cancer. Int. J. Cancer. 1980; 26: 429–33.
Green, L. M, Reade, J. L., Ware, C.F. Rapid Colormetric Assay for Cell Viability: Application to the Quantitation of Cytotoxic and Growth Inhibitory Lymphokines. J. Immunol. Methods 1984; 70: 257–268.
Danis, V. A., Harries, A. D., Heatley, R. V.In vitro immunoglobulin secretion by normal human gastrointestinal mucosa tissues, and alterations in patients with inflammatory bowel disease. Clin. Exp. Immunol. 1984; 56: 159–166.
Crabtree, J. E, Heatley, R. V., Losowsky, M. S. Glycoprotein synthesis and secretion by cultured small intestinal mucosa in calico disease. Gut 1989; 30: 1339–1343.
Peterson, G. L. A simplification of the protein assay method of Lowry et al which is more generally applicable. Anal. Biochem. 1987; 83: 346–56.
Crabtree, J. E., Shallcross, T. M., Heatley, R. V., Wyatt, J. I. Mucosa tumour necrosis factor a and interleukin-6 in patients withHelicobacter pylori associated gastritis. Gut 1991; 32: 1473–77.
Meager, A. Antibodies against interferons: characterization of interferons and immunoassays. In: Clemens, M., Morris, A., Gearing, A., eds. Lymphokines and Interferons. IRL Press, Oxford 1987; 105–127.
Shahi, C. N., Fan, X. J., Chua, A., McDevitt, J., Weir, D. G., Keeling, P. W. N., Kelleher, D. Defective antigen-specific responses toHelicobacter pylori antigens in patients with gastricH. pylori colonisation. Gastroenterology 1992; 102: A694.
O’Garra, A. Peptide Regulatory Factors. Interleukins and the immune system 2. Lancet. 1989; 6: 1003–1005.
Mai, U. E. H., Perez-Perez, G. I., Wahl, L. M., Wahl, S. M., Blaser, M. J., Smith, P. D. Soluble surface proteins fromHelicobacter pylori activate monocytes/macrophages by lipopolysaccharide-independent mechanism. J. Clin. Invest. 1991; 87: 894–900.
Strickland, R. G., Mackay, I. R. A reappraisal of the nature and significance of chronic atrophic gastritis. Am. J. Dig. Dis. 1973; 18: 426–440.
Greenlaw, R., Sheahan, D. G., Deluca, V., Melter, D. A., Myerson, D., Myerson, P. Gastroduodenitis: a broader concept of peptic ulcer disease. Dig. Dis. Sci. 1980; 25: 660–672.
Gilman, R. J., Leon-Barua, R., Koch, J. Rapid identification ofpylori Campylobacter in Peruvians with gastritis. Dig. Dis. Sic. 1986; 31: 1089–1094.
Blaser, M.J. Gastric Campylobacter-like organisms, gastritis and peptic ulcer disease. Gastroenterol. 1987; 93: 371–383.
Karttunen, R. Blood lymphocyte proliferation, cytokine secretion and appearance of T cells with activation surface markers in cultures withHelicobacter pylori. Comparison of the responses of subjects with and without antibodies toH. pylori. Clin. Exp. Immunol. 1991; 83: 396–400.
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Fan, X.J., Chua, A., O’Connell, M.A. et al. Interferon-gamma and Tumour Necrosis Factor Production in Patients withHelicobacter pylori Infection. I.J.M.S. 162, 408–411 (1993). https://doi.org/10.1007/BF02996319
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DOI: https://doi.org/10.1007/BF02996319