Abstract
This paper describes the isolation of isoguanosine fromCroton tiglium L. and its cytotoxic effect against several tumor cell lines in culture and newly reports that isoguanosine has an antitumor activity against implanted S-180 ascitic tumor mice. Isoguanosine is effective at the dose of 24 mg/kg/day×5, with T/C value of 168%. Isoguanosine inhibits the growth of S-180 and Ehrlich solid tumor in mice at the optimal doses of 96 mg/kg/day×12 and 48 mg/kg/day×12, with 1-T/C values of 65% and 60% respectively.
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References cited
Buell, M. V. and Perkins, M. E., Cryst. guanine nucleoside.J. Biol. Chem., 72, 745–748 (1927).
Cherbuliez, E. and Bernhard, K., Croton seed(1)crotonoside.Helv. Chim. Acta., 15, 464, 978–982 (1932).
Divaker, K. J., Colin, B. R., Yogesh, S. S. and Karl, A. D., Conversion of guanosine into isoguanosine and derivatives.J. Chem. Soc. Perkin Trans I, 771–774 (1991).
Fuhrman, A. F., Geraldin, J. F., Ronald, J. N. and Harry, S. M., Isoguanosine; isolation from an animal.Science, 212, 557–558 (1981).
Hagen, C., Analysis in the variation in lymphocyte response to PHA (phytohemagglutinin) in normal subjects.Biochem. Biophys. Acta., 293, 105–110 (1973).
Huang, M., Shimizu, H. and Daly, J. W., Accumulation of cyclic adenosine monophosphate in incubated slices of brain tissue.J. Med. Chem., 15, 462–468 (1972).
Karon, M. and Shirakawa, S., Rocus of action of 1-β-D-arabinofuranosyl cytosine in the cell cycles.Cancer Res., 29, 687–696 (1969).
Kim, C. W., Moon, J. C. and Kim, J. B., Cytotoxic effects of extract (CP-2) from the mixture of Coptis andCroton tiglium L. of the various tumor cell-lines.The Korean Central Journal of Medicine, 58(3), 177–184 (1993).
Kim, J, H., Lee, S. J., Han, Y. B. and Kim, J. B., Identification of active compounds fromCroton tiglium andCoptis japonica aqeous mixture (CP2) and studies of it's cytotoxicity.Kor. J. Pharmaeogn., 38(1), 31–37 (1994).
Kyoichi, S., Toshitaka, M. and Sueo, M. U., Antitumor activity and hematotoxicity of a new, substituted dihydrobenzo., FK 973, in mice. Cancer Res., 48, 1168–1172 (1988).
Lowry, B. A. and Brown, G. B., The utilization of purine nucleosides for nucleic acid synthesis in the rat.J. Biol. Chem.,197, 591 (1952).
Mosmann, T. Rapid calorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity.J. Immumol. Methods, 65, 55–63 (1983).
Pike, L. M. and Rottman, F., The determination of 2′-O-methyl nucleosides in RNA.Anal. Biochem., 61, 367–378 (1974).
Skipper, H. E., Montgomery, J. A., Thomson, J. R. and Schabel, F. M., Structure-activity relations and crossresistance observed on evaluation of a series of purine analogs against exptl. neoplasms.Cancer Res., 19, 425 (1959).
Vasu, N. and David, A. Y., A New Synthesis of Isoguanosine.J. Org. Chem., 50, 406–408 (1985).
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Kim, J.H., Lee, S.J., Han, Y.B. et al. Isolation of isoguanosine fromCroton tiglium and its antitumor activity. Arch. Pharm. Res. 17, 115–118 (1994). https://doi.org/10.1007/BF02974234
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DOI: https://doi.org/10.1007/BF02974234