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Fabrication of protein a-viologen hetero Langmuir-Blodgett film for fluorescence immunoassay

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Abstract

Protein A molecular thin film was fabricated as a platform of antibody-based biosensor. For the immobilization of the protein A thin film, a viologen multilayer was built up using the Langmuir-Blodgett (LB) technique, and then, protein A was adsorbed on the viologen LB film by an electrostatic interaction force, which was formed as a hetero-film structure. For the deposition of viologen, surface pressure area (π-A) isotherm was investigated. The fabricated protein A-viologen hetero LB film was investigated using atomic force microscopy (AFM). Using the developed molecular film, antibody immobilization and fluorescence measurement was carried out.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, Korea

    Woochang Lee, Bum Suk Chun, Byung-Keun Oh, Won Hong Lee & Jeong-Woo Choi

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  1. Woochang Lee
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  2. Bum Suk Chun
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  3. Byung-Keun Oh
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  4. Won Hong Lee
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  5. Jeong-Woo Choi
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Correspondence to Jeong-Woo Choi.

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Lee, W., Chun, B.S., Oh, BK. et al. Fabrication of protein a-viologen hetero Langmuir-Blodgett film for fluorescence immunoassay. Biotechnol. Bioprocess Eng. 9, 241–244 (2004). https://doi.org/10.1007/BF02942337

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  • DOI: https://doi.org/10.1007/BF02942337

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