Abstract
The relationship between inflammatory bowel disease and microorganisms was evaluated. The presence ofCandida albicans-specific IgM and IgG antibodies in serum samples and the presence ofC. albicans in stool and colonal mucosa samples of the patients did not exhibit any significant difference between 21 patients in active stage and 15 patients in remission of ulcerative colitis (UC) (compared with 19 control patients). The invasion of yeast cells to the colonal mucosa was demonstrated by detectingC. albicans DNA using specific PCon1, PCon2, and PspA2 primers in PCR assay. Eighteen of 36 patients (50 %) were found to be DNA positive while in 19 controls only 4 (21 %) were found to be positive. The presence of DNA in the association of the positive serological reactivity is suggested as an important diagnostic marker of UC.
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Bernstein C.N., Nayar G., Hamel A., Blanchard J.F.: Study of animal-borne infections in the mucosas of patients with inflammatory bowel disease and population-based controls.J.Clin.Microbiol. 41, 4986–4990 (2003).
Bernstein C.N., Blanchard J.F., Rawsthorne P., Collins M.T.: Population-based case control study of seroprevalence ofMycobacterium paratuberculosis in the patients with Crohn’s disease and ulcerative colitis.J.Clin.Microbiol. 42, 1129–1135 (2004).
Bujdáková H., Melkusová S., Soji I., Mokráš M., Mikami Y.: Discrimination betweenCandida albicans andCandida dubliniensis isolated from HIV-positive patients by using commercial method in comparison with PCR assay.Folia Microbiol. 49, 484–490 (2004).
van Burik J.A., Myerson D., Schreckhise R.W., Bowden R.A.: Panfungal PCR assay for detection of fungal infection in human blood specimens.J.Clin.Microbiol. 30, 1169–1175 (1998).
Burke V., Gracey M.: An experimental model of gastrointestinal candidiasis.J.Med.Microbiol. 13, 193–210 (1980).
Bykov V.L.: Velocity ofCandida albicans invasion into host tissues.Mycoses 34, 293–296 (1991).
Cole G.T., Seshan K.R., Pope L.M., Yancey R.J.: Morphological aspects of gastrointestinal tract invasion byCandida albicans in the infant mouse.J.Med.Vet.Mycol. 26, 173–185 (1988).
Curran F.T., Youngs D.J., Allan R.N., Keighley M.R.B.: Candidacidal activity of Crohn’s disease neutrophils.Gut 32, 55–60 (1991).
Davidson I.W., Lloyd R.S., Whorwell P.J., Wright R.: Antibodies to mouse in patients with Crohn’s disease, ulcerative colitis and celiac disease.Clin.Exp.Immunol. 35, 147–148 (1979).
Einsele H., Hebart H., Roller G., Loffler J., Rothenhofer I., Muller C.A., Bowden R.A., van Burik J.A., Engelhard D., Kanz L., Schumacher U.: Detection and identification of fungal pathogens in blood by using molecular probes.J.Clin.Microbiol. 35, 1353–1360 (1997).
Eisen D.P., Bartley P.B., Hope W., Sigmundsdottir G., Pehrson C., Larsson L., Christensson B.: Urined-arabinitol/l-arabinitol ratio in diagnosingCandida infection in patients with hematological malignancy and HIV infection.Diagn.Microbiol.Infect.Dis. 42, 39–42 (2002).
Hamal P., Dostál J., Raclavský V., Krylová M., Pichová I., Hrušková-Heidingsfeldová O.: Secreted aspartate proteinases, a virulence factor ofCandida spp.: occurrence among clinical isolates.Folia Microbiol. 49, 491–496 (2004).
Hendrickson B.A., Gokhale R., Cho J.H.: Clinical aspects and patholophysiology of inflammatory bowel disease.Clin.Microbiol.Rev. 15, 79–94 (2002).
Hermann P., Forgács K., Gál E., Lenkey B., Nagy G., Rozgonyi F.: Effects of alkali metal ions on some virulence traits ofCandida albicans.Folia Microbiol. 48, 173–176 (2003).
Kalkanci A., Kustimur S., Gunes I., Otlu B.: Diagnosis of invasiveCandida infections in neutropenic patients by polymerase chain reaction. (In Turkish)Turk.J.Infect. 17, 171–174 (2003).
Khatib R., Riederer K.M., Ramanathan J., Baran J. Jr.: Faecal fungal flora in healthy volunteers and inpatients.Mycoses 44, 151–156 (2001).
Kozel T.R., MacGill R.S., Percival A., Zhou Q.: Biological activities of naturally occurring antibodies reactive withCandida albicans mannan.Infect.Immun. 7, 209–218 (2004).
Kroflach P., Park B.H., Cunnigham R., Weiser M.M., Albini B.: Serum antibodies to cow’s milk in ulcerative colitis and Crohn’s disease.Gastroenterology 92, 479–485 (1987).
Kustrzeba-Wojcicka I., Golczak M.: Enolase fromCandida albicans — purification and characterization.Comp.Biochem.Physiol.B Biochem.Mol.Biol. 126, 109–120 (2000).
Main J., McKenzie H., Yeaman G.R., Kerr M.A., Robson D., Pennington C.R., Parratt D.: Antibody toSaccharomyces cerevisiae (baker’s yeast) in Crohn’s disease.Brit.Med.J. 297, 1105–1106 (1988).
McKenzie H., Main J., Pennigton C.R., Parratt D.: Antibody to selected strains ofSaccharomyces cerevisiae (baker’s and brewer’s yeast) andCandida albicans in Crohn’s disease.Gut 31, 536–538 (1990).
McKenzie H., Parratt D., Main J., Pennington C.R.: Antigenic heterogeneity of strains ofSaccharomyces cerevisiae andCandida albicans recognized by serum antibodies from patients with Crohn’s disease.FEMS Microbiol.Immunol. 89, 219–224 (1992).
Nagy F., Szenasi Z., Szöllösy E., Kiss I., Varro V.: Determination and kinetics of antibody response toCandida albicans in ulcerative colitis.Hepato-Gastroenterol. 36, 182–184 (1989).
Nichterlein T., Buchheidt D., Hein A., Becker K.P., Mosbach K., Kretschmar M.: Comparison of glucan detection and galactomannan enzyme immunoassay in gastrointestinal and systemic murine candidiasis.Diagn.Microbiol.Infect.Dis. 46, 103–108 (2003).
Novák A., Vágvölgyi C., Pesti M.: Characterization ofCandida albicans colony-morphology mutants and their hybrids.Folia Microbiol. 48, 203–210 (2003).
Novák A., Vágvölgyi C., Emödy L., Pesti M.: Characterization ofCandida albicans colony morphological mutants and their hybrids by means of RAPD-PCR, isoenzyme analysis and pathogenicity analysis.Folia Microbiol. 49, 527–533 (2004).
Pilipčinec E., Dorko E., Tkáčiková L’., Wantrubová A., Bugarský A.: The detection of anti-Candida antibodies on experimental animal models.Folia Microbiol. 48, 555–560 (2003).
Seibold F., Stich O., Hufnagl R., Kamil S., Scheurlen M.: Anti-Saccharomyces cerevisiae antibodies in inflammatory bowel disease: a family study.Scand.J.Gastroenterol. 36, 196–201 (2001).
Sendid B., Colombel J.F., Jacquinot P.M., Faille C., Fruit J., Cartot A., Lucidarme D., Camus D., Poulain D.: Specific antibody response to oligomannosidic epitopes in Crohn’s disease.Clin.Diagn.Lab.Immunol. 3, 219–226 (1996).
Trost A., Graf B., Eucker J., Sezer O., Possinger K., Gobel U.B., Adam T.: Identification of clinically relevant yeasts by PCR/RFLP.J.Microbiol.Meth. 56, 201–211 (2004).
Verduyn Lunel F.M., Voss A., Kuijper E.J., Gelinck L.B., Hoogerbrugge P.M., Liem K.L., Kullberg B.J., Verweij P.E.: Detection of theCandida-antigen mannan in cerebrospinal fluid specimens from patients suspected of havingCandida meningitis.J.Clin.Microbiol. 42, 867–870 (2004).
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Kalkanci, A., Tuncer, C., Degertekin, B. et al. Detection ofCandida albicans by culture, serology and PCR in clinical specimens from patients with ulcerative colitis: Re-evaluation of an old hypothesis with a new perspective. Folia Microbiol 50, 263–267 (2005). https://doi.org/10.1007/BF02931575
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DOI: https://doi.org/10.1007/BF02931575