Summary
To investigate whether the TGF-β1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-β1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-β1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-β1 protein expression specific for pMAMTGF-β1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-β1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.
Similar content being viewed by others
References
Borra T, Tamm E R, Zigler J S J R. Ocular adenovirus gene transfer varies in efficiency and inflammatory response. Invest Ophthalmol Vis Sic, 1996, 37: 1282
Osusky R, Jiang M, Büchi E R et al. beta-Galactosidase transgene expression in transplanted rabbit retinal pigment epithelial cell in vivo. Graefe's Arch Clin Exp Ophthalmol, 1995, 223: 220–225
Li T, Davidson B L. Phenotype correction in retinal pigment epithelium in murine mucopolysaccharidosis VII by adenovirus-mediated gene transfer. Proc Natl Acad Sci USA, 1995, 92: 7700
Pepose J S, Leib D A. Herpes simplex viral vectors for therapeutic gene delivery to ocular tissues: recent break-throughs in the molecular genetics of ocular diseases. Invest Ophthalmol Vis Sic, 1994, 35: 2662
Crystal R G. Transfer of genes to humans: early lessons and obstacles to success. Science, 1995, 270: 404
Masuda I, Matsuo T, Yasuda T et al. Gene transfer with liposomes to the intraocular tissues by different routes of administration. Invest Ophthalmol Vis Sic, 1996, 37: 1914
Smith J G, Walzem R L, German J B. Liposomes as agents of DNA transfer. Biochem Biophys Acta, 1993, 1154: 327
Farhood P L, Gao X, Son K et al. Cationic liposomes for direct gene transfer in therapy of cancer and other diseases. Ann NY Acad Sci, 1994, 716: 23
Oshima Y, Sakamoto T, Yamanka Tet al. Targeted gene transfer to corneal endothelium in vivo by electric pulse. Gene Ther, 1998, 5: 1347
Kahn CR, Young E, Lee IHet al Human corneal epithelial primary cultures and cell lines with extended life span:in vitro model for ocular studies. Invest Ophthalmol Vis Sci, 1993, 34: 3429
Bradshaw JJ, Obritsch WF, Cho BJet al. Ex vivo transduction of corneal epithelial progenitor cells using a retroviral vector. Invest Ophthalmol Vis Sic, 1999, 40: 230
Tsubota K, Inoue H, Hudo Ket al. Adenoviral-mediated gene transfer to the ocular surface epithelium. Exp Eye Res, 1998, 67: 531
Seitz B, Moreira L, Baktanian Eet al. Retroviral vector- mediated gene transfer into keratocytesin vitro andin vitro. Am J Ophthalmol, 1998, 126: 630.
Hart S L, Arancibia-Carcamo C V, Wolfert M Aet al. Lipid-mediated enhancement of transfection by a nonviral integrin-targeting vector. Hum Gene Ther, 1998, 9: 575
Shewring L, Collins L, Lightman S L et al. A nonviral vector system for efficient gene transfer to corneal endothelial cells via membrane integrins. Transplantation, 1997, 64: 763
Author information
Authors and Affiliations
Additional information
HUANG Qiong, female, born in 1977, Doctor in Charge
This project was supported by a grant from the Natural Sciences Foundation of Hubei Province (N0. 97J070).
Rights and permissions
About this article
Cite this article
Qiong, H., Yanhua, H., Fagang, J. et al. Experimental study of plasmid TGF-β1 DNA gene transfer with lipofectamine into rabbit corneal epithelial cellsin vitro. Current Medical Science 22, 62–65 (2002). https://doi.org/10.1007/BF02904792
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02904792