Abstract
The development of neutralizing antibodies against bacteriophage T 2 in newborn rabbits was investigated. On the seventh day after antigen injection only antibodies of 19 S type were detected following the fractionation of serum on a saccharose density gradient. The neutralizing activity of these antibodies was heavily complement-dependent: the neutralizing capacity was completely destroyed by inactivation at 56° C for 30 min; however, it was possible to renew it by the addition of fresh complement. Antibodies of the 7 S type detected on the fifteenth day after immunization by ultracentrifugation, were also dependent on complement in their neutralizing capacity. Antibodies of the 7 S type produced on the 28th day after immunization were still partially dependent on complement; however, antibodies produced in the secondary response no longer required complement for their neutralizing activity. The obtained results indicate that in the course of the primary and secondary response to bacteriophage T 2, antibodies of 19 S and 7 S are gradually formed in the newborn with higher ability to neutralize the phage particle alone without the potentiating action of complement.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bauer, D. C., Mathies, M. J., Stavitsky, A. B.:Sequences of synthesis of γ-1 macroglobulin and γ-2 globulin antibodies during primary responses to protein, Salmonella antigens and phage. J. exptl. Med. 117: 889, 1963.
Cowan, K. M.:Studies on the coliphage neutralizing activity of normal serum. J. Immunol. 88: 476, 1962.
Hájek, P.:Properties of neutralizing factors against T 2 and ΦX 174 phages present in normal sera. Fol. microbiol. 11: 290, 1966.
Hájek, P., Mandel, L.:Antibody response of young animals to bacteriophages of different immunological behaviour: ΦX 174 and T 2. Fol. microbiol. 11: 282, 1966.
Herriot, R. M., Barlow, J. L.:Preparation, purification and properties of E. coli virus T 2. J. gen. Physiol. 36: 17, 1952.
Kim, Y. B., Bradley, S. G., Watson, D. W.:Ontogeny of immune response. II. Characterization of 19 S γG and 7 S γG-immunoglobulins in the true primary and secondary responses in piglets. J. Immunol. 97: 189, 1966.
Muschel, L. H., Toussaint, A. J.:Studies on the bacteriophage neutralizing activity of serums. II. Comparison of normal and immune phage neutralizing antibodies. J. Immunol. 89: 35, 1962.
Pernis, B., Ghezzi, I., Turri, M.:Properties of phage-neutralizing antibodies produced by new-born rabbits. Nature 197: 807, 1963.
Styk, B.:Cofactor and specific antibodies against influenza viruses. III. The potentiating effect of cofactor on specific antibodies of early immune and of hyperimmune sera and the differences in the character of these antibodies. Acta virol. 6: 327, 1962.
Styk, B., Závada, J., Hána, L.:The role of non-specific serum factors in the antibody response to the ΦX 174 bacteriophage. Fol. microbiol. 9: 263, 1964.
Styk, B., Hána, L.:Formation of 19 S and 7 S type viral antibodies. The role and nature of “antibody cofactor”. In: Molecular and cellular basis of antibody formation. Publ. House Czechoslov. Acad. Sci. Prague 1965.
Svehag, S. E., Mandel, B.:Production and properties of polivirus neutralizing antibody by rabbit origin. Virology 18: 508, 1962.
Šterzl, J., Mandel, L., Miler, I., Říha, I.:Development of immune reactions in the absence or presence of an antigenic stimulus. In: Molecular and cellular basis of antibody formation. Publ. House Czechoslov. Acad. Sci. Prague 1965.
Uhr, J. W., Finkelstein, M. S., Franklin, E. C.:Antibody response to bacteriophage ΦX 174 in non-mammalian vertebrates. Proc. Soc. exptl. Biol. Med. 111: 13, 1962.
Uhr, J. W., Finkelstein, M. S.:Antibody formation. IV. Formation of rapidly and slowly sedimenting antibodies and immunological memory to bacteriophage ΦX 174. J. exptl. Med. 117: 457, 1963.
Yoshino, K., Taniguchi, S.:Studies on the neutralization of herpes simplex virus. I. Appearance of neutralizing antibodies having different grades of complement requirement. Virology 26: 44, 1965.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hájek, P. Neutralization of bacterial viruses by antibodies of young animals. Folia Microbiol 14, 165–170 (1969). https://doi.org/10.1007/BF02892886
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02892886