Summary
The stereological algorithms for the conversion of the profile-size frequency distribution (PSFD) observed in histological section to the spheresize frequency distribution (SSFD) in three-dimensional space was developed to a computer program and its practical application was described in this paper. The reconstructions of SSFD were based on the data of PSFD of A-and B-cells measured in sections of the pancreatic islets from ophiocephalus argus, toad, chicken, cat, rabbit and rat. The sizes of both profile and sphere were expressed by their caliper diameters. The experimental results showed that the patterns of SSFD of A-and B-cells from six animal species were somewhat different, and that all the peaks of them, however, occurred in the range from 6 to 12 Urn in caliper diameter.
Similar content being viewed by others
References
Wickseil SD. The corpuscle problem. A mathematical study of a biometric problem. Biometrica 1926;17:84
Wicksell SD. The corpuscle problem. Second memoir. Case of ellipsoidal corpuscles. Biometrica 1926;18:152
Gruz-Orive LM, Particle size-shape distributions: the general spheroid problem. I. Mathematical model. J Microsc 1976;107:235
Gruz-Orive LM. Farticle size-shape distributions: the general spheroid problem. II. Stochastic model and practical guide. J Microsc 1978;112:153
Weibel ER, Stereological Methods. Vol. I, Practical methods for biological morphometry Academic Press Inc.: London, 1979;189
Helman B. The numerical distribution of the islets of Langerhans at different ages of the rat. Acta Endocrinologica 1959;32:63
Weibel ER, et al. Integrated stereological biochemical studies on hepatocytic membrane. II. Correction of section thickness effect on volume and surface density estimates. J Cell Biol 1978;77: 584
Weibel ER, et al. Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereologigic methods and normal morphometric data for rat liver. J Cell Biol 1969;42:68
Bolender RP, et al. Integrated stereological and biochemical studies en hepatocytic membranes. I. Membrane recoveries in subcellular fractions. J Cell Biol 1978;77:565
Bao HF. Structure characteristics of QMSOC and the relevant operators. J Tongji Med Univ 1989;9:235
Bao HF. The new function of quantitatively medicine simulating and operating by computer (QMSOC). J Tongji Med Univ 1990;10:52
Bao HF. Information medicine and Its primary functions of exploitation of medical resources. J Tongji Med Univ 1991;11:59
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhu-jun, A., Han-fei, B. & Qing-tang, W. Reconstruction of the frequency distribution of A-and B-cell size in the pancreatic islets from several animal species — An important stereological operation in QMSOC. Journal of Tongji Medical University 11, 214–219 (1991). https://doi.org/10.1007/BF02888154
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02888154