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ATP evokes calcium transients in single pulmonary artery endothelial cell in primary cultures

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Summary

Effects of ATP on cytosolic free calcium ([Ca2+]i) in single porcine pulmonary artery endothelial cell were studied. Using a dual-wavelength excitation microflurometry. it was found that ATP evoked a rapid transient in [Ca2+]i which was then followed by a maintained elevation of [Ca2+]i. The removal of extracellular Ca2+ abolished the maintained plateform, but exerted no obvious effect on the initial-transient. These results suggest that ATP stimulates both calcium release from intracellular calcium pool(s) and calcium influx across the plasma membrane from extracellular space.

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References

  1. Martin W, Cusack N J, Carleton J Set al. Specificity of P2-purinoreceptor that mediates endothelium-dependent relaxation of the pig aorta. Eur J Pharmacol, 1985; 108: 295

    Article  PubMed  CAS  Google Scholar 

  2. Peach M J, Singer H A, Izzo N Jet al. Role of calcium in endothelium dependent relaxation of arterial smooth muscle. Am J Cardiol, 1987; 59: 35A

    Article  PubMed  CAS  Google Scholar 

  3. 1992; 8(4): 447

  4. Grynkiewicz G, Poenie M, Tsien R Y. A new generation of Ca2+ indicators with greatly improved fluorescent properties. J Biol Chem, 1985; 260: 3440

    PubMed  CAS  Google Scholar 

  5. Hu Qing-hua, Wang Di-xun. Hypoxia increases cytosolic free calcium in porcine pulmonary artery endothelial cells. J Tongji Med Uniy, 1993; 13(1): 14

    CAS  Google Scholar 

  6. Elliott S J, Schilling W P. Carmustine augments the effects of tert-Buty(hydro-peroxide) on calcium signaling in cultured pulmonary artery endothelial cells. J Biol Chem, 1990; 265 (1): 103

    PubMed  CAS  Google Scholar 

  7. Danthuluri N R, Cybulsky M I, Broch T A. Ach-induced calcium transients in primary cultures of rabbit aortic endothelial cells. Am J Physiol, 1988; (Heart. Circ Physiol 24): H1549

    Google Scholar 

  8. Clotilde Randriamampita, Tsien R Y. Emptying of intracellular Ca2+ stores releases a novel small messenger that stimulates Ca2+ influx. Nature, 1993; 364: 809

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathophysiology, Tongji Medical University, Wuhan

    Hu Qing-hua, Wang Di-xun, Zhang Yu, Wang **-xi & Chen Rong-shan

  2. Department of Chemistry, Nan** University, Nan**

    Zhang Yu, Wang **-xi & Chen Rong-shan

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  1. Hu Qing-hua
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  2. Wang Di-xun
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  3. Zhang Yu
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  4. Wang **-xi
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  5. Chen Rong-shan
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Qing-hua, H., Di-xun, W., Yu, Z. et al. ATP evokes calcium transients in single pulmonary artery endothelial cell in primary cultures. Journal of Tongji Medical University 14, 42–44 (1994). https://doi.org/10.1007/BF02888057

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  • DOI: https://doi.org/10.1007/BF02888057

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