Summary
In order to explore the effects of pressure on trabecular meshwork cells, bovine trabecular meshwork cells were culturedin vitro and subjected to different levels of hydrostatic pressure. The cellular morphology, ultrastructure and phagocytosis were studied with inverted phase-contrast microscopy, light microscopy and transmission electron microscopy, etc. It was found that the cells subjected to 2. 0 kPa or 2. 67 kPa for 48 h had no remarkable difference as compared with the controls in terms of parameters observed. Those under 4. 0 kPa for 24 h showed slight changes in structure and a mild decrease in phagocytic function. The damage appeared more severe if the pressure was higher or lasted longer. From the above we conclude that trabecular meshwork cells can only bear pressure below a certain level. They may be destroyed structurally or impaired functionally by pressure over this level.
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Dandona L, Hendrickson A, Quigley H A. Selective effects of experimental glaucoma on axonal transport by retinal ganglion cells to the dorsal lateral geniculate nucleus. Invest Ophthalmol Vis Sci, 1991, 32: 1593
Knorr H L, Handel A, Naumann G O. Morphometric and qualitative changes in corneal endothelium in primary chronic open angle glaucoma. Fortschr Ophthalmol, 1991, 88: 118
Johnstone M A, Grant W M. Pressure-dependent change in structure of the aqueous outflow system of human and monkey eye. Am J Ophthalmol, 1973, 75:365
Drecoll L, Shimizu T. Quantitative analysis of “plague material” in the inner and outer wall of Schlemm’s cannai in normal and glaucomatous eye. Exp Eye Res, 1986, 42:443
Tanihara H, Ohuchi T, Yoshimura Net al. Heterogeneous response in calcium signaling by adrenergic and cholinergic stimulation in cultured bovine trabecular cells. Exp Eye Res, 1991, 52:393
Grierson I, Day J, Unger W Get al. Phagocytosis of latex microspheres by bovine meshwork cells in culture. Graefe’s Arch Clin Exp Ophthalmol, 1986, 224:536
Qiang W, Houren W, Zhgenjun Fet al. Effects of norfloxacin and clondine in human trabecular meshwork cells in vitro. Graefe’s Arch Clin Exp Ophthalmol, 1994, 232:566
Alvarado J, Murphy C, Huster R. Trabecular meshwork cellularity in primary open angle glaucoma and nonglaucomatous normals. Ophthamology, 1984, 91: 564
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Fagang, J., Houren, W., Yuanshu, L. et al. Effects of hydrostatic pressure on cultured bovine trabecular meshwork cells. Current Medical Science 19, 319–322 (1999). https://doi.org/10.1007/BF02886973
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DOI: https://doi.org/10.1007/BF02886973