Abstract
The iodination efficiency of salmon GH(sGH) was 38.82%, using a modification of the chloramine-T method. The specific activity of the125I-sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low-capacity binding site in flounder liver. Long-term administration with exogenous GH can induce the up-regulation of hepatic GH receptor in total binding capacity though there was no significant difference in capacity of free binding sites of livers from control and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish, had more occupied binding sites.
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Contribution No.247 of Experimental Marine Biology Laboratory and 3798 of the Institute of Oceanology, Chinese Academy of Sciences.
Project 96-C01-05-04 supported by National “Ninth Five Year Plan Key Problem”.
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Liu, Zz., Wang, Jb., Xu, Yl. et al. Up-regulation of hepatic receptor for growth hormone in the flounder (Paralichthys olivaceus) after oral administration with exogenous GH. Chin. J. Ocean. Limnol. 19, 135–140 (2001). https://doi.org/10.1007/BF02863037
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DOI: https://doi.org/10.1007/BF02863037