SpringerLink
Log in

Plantlets regeneration from root explants ofRobinia pseudoacacia L. by suspension culture

  • Original Articles
  • Published:
Journal of Forest Research

Abstract

A rapid and efficient method for the regeneration of plantlets from root explants ofRobinia pseudoacacia L. by suspension culture was established. The roots taken from aseptically grown 15-day-old seedlings were used as explants. It was determined that photoperiodicity was necessary for root proliferation, and that the promotive effect of IAA (3-indoleacetic acid) on root proliferation was better than that of IBA (3-indolebutyric acid). The roots cultured in 1/2 MS liquid medium containing 3 µM IAA and 1% sucrose at 25°C under 16-hour photoperiod with 50 µmol m−2s−1 PPFD (photosynthetic photon flux density) shaking at 100 times/min reciprocally showed high efficiency for root proliferation. BAP (6-benzylaminopurine) was found to be essential to induce adventitious shoots from the roots, and the roots cultured in the medium supplied with 3 µM BAP combined with 1–6 µM IAA for 3 weeks under the same conditions as in the root proliferation period were most suitable for adventitious shoot inducement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (France)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  • Arrillaga, I. (1993) Regenerating plant fromin vitro culture of black locust cotyledon and leaf explants. Hort. Sci. 28: 942–945.

    Google Scholar 

  • Arrillaga, I., Tobolski, J. J., and Merkle, S. A. (1994) Advances in somatic embryogenesis and plant production of black locust. Plant Cell Rep. 13: 171–175.

    Article  CAS  Google Scholar 

  • Barghchi, M. (1987) Mass clonal propagation ofRobinia pseudoacacia. Plant Sci. 53: 183–189.

    Article  CAS  Google Scholar 

  • Cella, R. and Iadarola, P. (1983) Characterization of carrot cell lines resistant to 5-methyltryptophan obtained by irradiating suspension culture with UV-light. Plant Sci. Lett. 29: 327–337.

    Article  CAS  Google Scholar 

  • Chalupa, V. (1983)In vitro propagation of willows (Salix spp.), European mountain ash (Sorbus ancuparia L.) and black locust (Robinia pseudoacacia L.). Biol. Plant 25: 305–307.

    Google Scholar 

  • Chalupa, V. (1987a) Effect of bezylaminopurine and thidiazuron onin vitro shoot proliferation ofTilia cordata Mill.,Sorbus ancuparia L. andRobinia pseudoacacia L. Biol. Plant 29: 425–429.

    CAS  Google Scholar 

  • Chalupa, V. (1987b) Vegetative propagation of broad-leaved woody species by cuttings and by anin vitro method. Lesnictví 33: 501–510.

    Google Scholar 

  • Chalupa, V. (1988)In vitro propagation of small-leaved linden (Tilia cordata), black locust (Robinia pseudoacacia) and mountain ash (Sorbus ancuparia) and growth of trees cultivatedin vitro. Lesnictví 34: 705–720.

    Google Scholar 

  • Chalupa, V. (1991) Somatic embryogenesis and plant regeneration inPicea, Quercus, Betula, Robinia, Fagus andAesculus. Commun. Inst. For. Cechosloveniae 15: 133–148.

    Google Scholar 

  • Chen, R., Gyokusen, K., and Saito, A. (1996) Screening of NaCl-tolerantRobinia pseudoacacia L. callus throughin vitro culture. Bull. Kyusyu Univ. For. 75: 15–26. (in Japanese with English summary)

    Google Scholar 

  • Civínova, B. and Sladký, Z. (1990) Stimulation of the regeneration capacity of tree shoot segment explantsin vitro. Biol. Plant. 32: 407–413.

    Google Scholar 

  • Davis, J. M. and Keathley, D. E. (1985) Regeneration of shoots from leaf disk explants of black locust,Robinia pseudoacacia L. Proceedings 4th North Central Tree Improvement Conference, East Lansing, MI, Ang. 12–14, 29–34.

  • Davis, J. M. and Keathley, D. E. (1987a) Toward efficient clonal propagation of mature black locust trees using tissue culture. Nitrogen Fixing Tree Res. Rep. 5: 57–58.

    Google Scholar 

  • Davis, J. M. and Keathley, D. E. (1987b) Differential responses toin vitro bud culture in matureRobinia pseudoacacia L. (black locust). Plant Cell Rep. 6: 431–434.

    CAS  Google Scholar 

  • Davis, J. M. and Keathley, D. E. (1988)In vitro propagation of a black locust tree with an unusual phenotype. Nitrogen Fixing Tree Res. Rep. 6: 65–67.

    Google Scholar 

  • Davis, J. M. and Keathley, D. E. (1989) Detection and analysis of T-DNA in crown gall tumors and kanamycin-resistant callus ofRobinia pseudoacacia. Can. J. For. Res. 19: 1118–1123.

    Article  CAS  Google Scholar 

  • Deguchi, M., Gyokusen, K., and Saito, A. (1994) Propagation of plantlets by the culture of multiple shoots and roots induced from callus originating in the cambium ofRobinia hispida L. J. Jpn. For. Soc. 74: 346–354. (in Japanese with English summary)

    Google Scholar 

  • Flowers, T. J., Hajibagheri, M. A., and Clipson, N. J. W. (1986) Halophytes. The Q. Rev. Biol. 61: 313–337.

    Article  Google Scholar 

  • Fujiwara, A. and Ojima, K. (1955) Physiological studies of plant roots (part 1) — Influences of some environmental condition on growth of isolated roots of rice plant and wheat. J. Sci. Soil Manure, Jpn. 26: 295–298. (in Japanese with English summary)

    Google Scholar 

  • Fukuda, T. (1994) Tissue culture of black locust. Plant Cell Technol. 16: 61–65. (in Japanese with English summary)

    Google Scholar 

  • Fukuda, T. and Koumoto, M. (1986) Studies on tissue culture of tree — cambium X: Lignin-carbohydrate complex in suspension-cultured cells ofRobinia pseudoacacia. Mokuzai Gakkaishi Jpn. 32: 827–832.

    Google Scholar 

  • Gebhardt, C., Schnebli, V., and King, P. J. (1981) Isolation of biochemical mutants using haploid mesophyll protoplasts ofHyoscyamus muticus. Planta 153: 81–89.

    Article  CAS  Google Scholar 

  • Han, K. H. and Keathley, D. E. (1988) Isolation and culture of protoplasts from tissue of black locust (Robinia pseudoacacia L.). Nitrogen Fixing Tree Res. Rep. 6: 68–70.

    Google Scholar 

  • Han, K. H., Davis, J. M., and Keathley, D. E. (1990) Differential responses persist in shoot explants regenerated from callus of two mature black locust trees. Tree Physiol. 6: 235–240.

    PubMed  Google Scholar 

  • Han, K. H., Keathley, D. E., and Gordon, M. P. (1993a) Cambial tissue culture and subsequent shoot regeneration from mature black locust (Robinia pseudoacacia L.). Plant Cell Rep. 12: 185–188.

    Article  Google Scholar 

  • Han, K. H., Keathley, D. E., Davis, J. M., and Gordon, M. P. (1993b) Regeneration of a transgenic woody legume (Robinia pseudoacacia L., black locust) and morphological alterations induced byAgrobacterium rhizogenes mediated transformation. Plant Sci. 88: 149–157.

    Article  Google Scholar 

  • Hu, Q. J. and Han, Y. F. (1985) A study on induction of plantlets from mature leaves ofRobinia pseudoacacia. Hereditas, China 7 (4): 20–21. (in Chinese with English summary)

    Google Scholar 

  • Kang, J. M., Kojima, K., Ide, Y., and Sasaki, S. (1996) Growth response to the stress of low osmotic potential, salinity and high pH in culture shoot of Chinese poplars. J. For. Res. 1: 27–29.

    Article  Google Scholar 

  • Kochba, J., Ben-Hayyim, G., Spiegel-Roy, P., Saad, S., and Neumann, H. (1982) Selection of stable salt-tolerant callus cell lines and embryos inCitrus sinensis andC. aurantiom. Z. Pflanzenphysiol. Bd. 106s: 111–118.

    Google Scholar 

  • Kuraishi, S. and Nishinari, N. (1973) An introduction to the principles of plant physiology. 626pp, Tokyo University, Tokyo. 379–389 (in Japanese)

    Google Scholar 

  • Merkle, S. A. and Wiecko, A. T. (1989) Regeneration ofRobinia pseudoacacia via somatic embryogenesis. Can. J. For. Res. 19: 285–288.

    Google Scholar 

  • Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  • Singh, J. and Siminovitch, D. (1976) A reliable method for the estimation of DNA in higher plant tissues. Anal. Biochem. 71: 308–317.

    Article  PubMed  CAS  Google Scholar 

  • Tarczynski, M. C., Jensen, R. G., and Bohnert, H. J. (1993) Stress protection of transgenic tobacco by production of the osmolyte mannitol. Science 259: 508–510.

    Article  PubMed  CAS  Google Scholar 

  • Vértesy, J. and Balla, G. (1985) Micropropagation ofRobinia pseudoacacia. MÉN Inform. Központja: 63–71.

  • Zou, D. B., Gyokusen, K., and Yahata, H. (1994) Regeneration of plantlets by callus culture ofRobinia pseudoacacia L. Sci. Bull. Fac. Agric. Kyushu Univ. 49: 1–7. (in Japanese with English summary)

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Forestry, Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan

    Ren Chen, Koichiro Gyokusen & Akira Saito

Authors
  1. Ren Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Koichiro Gyokusen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akira Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Chen, R., Gyokusen, K. & Saito, A. Plantlets regeneration from root explants ofRobinia pseudoacacia L. by suspension culture. J For Res 3, 161–165 (1998). https://doi.org/10.1007/BF02762138

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02762138

Key words

Search

Navigation