Abstract
The fiber-optic biosensor consisted of tubular enzyme reactor and two sensing parts was developed for the detection of organophosphorus compounds in a contaminated water. The organophosphorus compounds was measured by acetylcholinesterase entrapped by Ca-alginate gel on a silicon tube. The litmus dye was used as the indicator of color change due to the inhibition of organophosphorus compounds on the acetylcholinesterase. Transmittance change of light at the input and output parts of tubular enzyme reactor was detected. The biosensor has the linear analytical range of 0–1.0 ppm with response time of 5 minutes. The proposed kinetics for irreversible inhibition of organophosphorus compounds on AChE agreed well with the experimental data. The theoretical model of diffusion and reaction in enzyme membrane was presented to analyze the response of sensor signal. Based on the simulation results using the model, the optimal amount of enzyme loaded and substrate was obtained as 80 μg and 6 mM, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdel-Latif, M.S. and Guidbault, G.G.,“Fiber Optic-Based Biosensors Utilizing Immobilized Enzyme Systems”, Biosensor Technology: Fundamentals and Applications, Buck, R. P., Hatfield, W. E., Umana, M. and Bowden, E. F., eds., Marcel Dekker, New York, 1990.
Alfthan, K., Kenttamaa, H. and Zukale, T.,“Characterization and Semiquantitative Estimation of Organophosphorus Compounds Based on Inhibition of Cholinesterases”,Analytica Chimica Acta,217, 43 (1989).
Carome, E. F., Coghlan, G.A., Sukenik, C.N. and Zull, J.E., “Fiber Optic Evanescent Wave Sensing of Antigen-Antibody Binding”,Sensors and Actuators B,13–14, 732 (1993).
Choi, U. S. and Cho, K. M.,“Environmental Engineering”, Cheongmungak, Seoul, 1990.
Dumschat, C., Müller, H., Stein, K. and Schwedt, G.,“PesticideSensitive ISFET Based on Enzyme Inhibition”,Analytica Chimica Acta,252, 7 (1991).
Klaimer, S. M., Thomas, J. R. and Franels, J. C.,“Fiber-Optic Chemical Sensors Offer a Realistic Solution to Environmental Monitoring Needs”,Sensors and Actuators B,11, 81 (1993).
Leon-Gonzalez, M. E. and Townshend, A.,“Flow-Injection Determination of Paraoxon by Inhibition of Immobilized Acetylcholinesterase”,Analytica Chimica Acta,236, 267 (1990).
Maron, M. J.,“Numerical Analysis; A Practical Approach”, Macmillan, New York, 1982.
Metzler, C.M., Elfring, G.L. and McEwen, A.J.,“A Package of Computer Programs for Pharmacokinetic Modeling”,Biometrics,30, 562 (1974).
Perry, R. H. and Green, D.,“Perry’s Chemical Engineering’s Handbook”, McGraw-Hill, New York, 1984.
Skladal, P.,“Determination of Organophosphate and Carbamate Pesticides Using a Cobalt Phthalocyanine Modified Carbon Paste Electrode and a Cholinesterase Enzyme Membrane”,Analytica Chimica Acta,252, 11 (1991).
Takruni, I.A., Almuaibed, A.M. and Townshend, A.,“Flow-Injection Study of Inhibition and Reactivation of Immobilized Acetylcholinesterase: Determination of the Pesticides Paraoxon and Carbamoylcholin”,Analytica Chimica Acta,282, 307 (1993).
Trettnak, W., Hofer, M. and Wolfbeis, O. S.,“Applications of Optochemical Sensors for Measuring Environmental and Biochemical Quantities”, Sensors, Vol. 3, Gopel, W., Hesse, J. and Zemel, J. N., eds.,VCH, New York, 1991.
Trettnak, W., Reininger, F., Zinterl, E. and Wolfbeis, O.S., Fiber-Optic Remote Detection of Pesticides and Related Inhibitors of the Enzyme Acetylcholineesterase”,Sensors and Actuators B,11, 87 (1993).
Tyrrell, H. I. V. and Harris, K. R.,“Diffusion on Liquids”, Butterworths, London, 1984.
Villadsen, J. and Michelsen, M. L.,“Solution of Differential Equation Models by Polynomial Approximation”, Prentice-Hall, Englewood Cliffs, 1978.
Wittmann, C. and Schmid, R. D.,“Application of an Automated Quasi-Continuous Immuno Flow Injection System to the Analysis of Pesticide Residues in Environmental Water Samples”,Sensors and Actuators B,15–16, 119 (1993).
Westrin, B. A. and Axelsson, A.,“Diffusion in Gels Containing Immobilized Cells; A Critical Review”,Biotech. Bioeng.,38, 439 (1991).
Wolfbeis, O. S. and Koller, E.,“Fiber Optic Detection of Pesticides in Drinking Water”, Biosensors Application in Medicine, Environmental Protection and Process Control, Schmid, R. D. and Scheller, F., eds., GBF, New York, 1989.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Choi, JW., Min, J. & Lee, WH. Signal analysis of fiber-optic biosensor for the detection of organophosphorus compounds in the contaminated water. Korean J. Chem. Eng. 14, 101–108 (1997). https://doi.org/10.1007/BF02706068
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02706068