Abstract
A mathematical kinetic model was proposed to describe the cell growth and the emulsan production in batch cultivations ofAcinetobacter calcoaceticus RAG-1. Ethanol and phosphate concentrations were chosen as the key variables, which affected the cell growth and emulsan production in the batch cultivations. The cell growth was inhibited by high concentrations of ethanol and was slightly affected by intracellular phosphate level. And the emulsan production was related to the intracellular phosphate level dependent upon the extracellular phosphate concentration. Kinetic model for the cell growth was formulated using the ethanol inhibition term and the intracellular phosphate level. The relationship between extracellular and intracelluiar phosphate level was expressed by the concept of active transport. Kinetic model for the emulsan production was represented using growth-associated term and intracellular phosphate inhibition term. Release of emulsan was presumed as the primary release from the surface of viable cell and the secondary release by cell lysis. The model predicted the experimental results with good agreement.
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References
Abbott, B. J.,“Ethanol Inhibition of a Bacterium(Acinetobacter calcoaceticus) in Chemostat Culture”,J. Gen. Microbiol.,75, 383 (1973).
Abbott, B. J., Laskin, A. I. and McCoy, C. J.,“Growth ofAcinetobacter calcoaceticus on Ethanol,Appl. Microbiol.,25, 787 (1973).
Bramble, J. L., Graves, D. J. and Brodelius, P.,“Calcium and Phosphate Effects on Growth and Alkaloid Production inCoffea arabica: Experimental Results and Mathematical Model”,Biotechnol. Bioeng.,37, 859 (1991).
Choi, J. W., Choi, H. G. and Ixe, W. H.,“Effects of Ethanol and Phosphate on Emulsan Production byAcinetobacter calcoaceticus RAG-1”,J. Biotechnol.,45, 217 (1996a).
Choi, J. W., Choi, H. G., Lee, K. S. and Lee, W. H.,“Control of Substrate Feed Rate in a Fed-batch Cultivation ofAcinetobacter calcoaceticus RAG-1 Using Feedback-assisted Iterative Learning Algorithm ,.J. Biotechnol., in press (1996b).
Dubois, M., Gillies, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F., Colorimetric Method for Determination of Sugars and Related Substances,Anal. Chem.,28, 350 (1959).
Gutnick, D. L, Bayer, E. A., Rubinowitz, C., Pines, O., Shabtai, Y., Goldman, S. and Rosenberg, E.,“Emulsan Production inAcinetobacter RAG-1”, Proceeding of the Sixth International Fermentation Symposium, Advances in Biotechnology, Vol. 3, Fermentation Product, Moo-Young, M., Robinson, C. W. and Vezina, C., eds., Pergamon Press, Toronto, (1980).
Gutnick, D. L. and Shabtai, Y.,“Exopolysaccharide Bioemulsifier”, Biosurfactants and Biotechnology, Kosaric, N., Cairns, W. L. and Gray, N. C. C., eds., Marcel Dekker, Inc., New York, (1987).
IBM Corporation, Technical Publications Department, System/ 360 Scientific Subroutine Package Programmer’s Manual”, 5th ed., New York, (1970).
Jarman, T. R. and Pace, G. W.,“Energy Requirement for Microbial Exopolysaccharide Synthesis”,Arch. Microbiol.,137, 231 (1984).
Kosaric, N., Cairns, W. L. and Gray, N. C. C., Biotechnology and Surfactant Industry, Biosurfactants and Biotechnology, Kosaric, N., Cairns, W. L. and Gray, N. C. C., eds.. Marcel Dekker, Inc., New York, (1987).
Luong, J. H. T.,“Generalization of Monod Kinetics for Analysis of Growth Data with Substrate Inhibition”,Biotechnol. Bioeng.,29, 242 (1987).
Maron, M. J.,“Numerical Analysis: A Practical Approach’, Macmillan Publishing Co., New York, (1982).
Metzler, C. M., Elfring, G. L. and McEwen, A. J.,“A Package of Computer Programs for Pharmacokinetic Modeling”,Biometric.,30, 562(1974).
Mulchandani, A. and Luong, J. H. T., Microbial Kinetics Revisited”,Enzyme Microb. Technol,11,66(1989).
Pazoutova, S., Votruba, J. and Rehacek, Z.,“A Mathematical Model of Growth and Alkaloid Production in the Submerged Culture ofClaviceps purpurea”,Biotechnol. Bioeng.,23, 2837 (1981).
Reisfeld, A., Rosenberg, E. and Gutnick, D. L,“Microbial Degradation of Crude Oil: Factors Affecting the Dispersion in Sea Water by Mixed and Pure Culture”,Appl. Microbiol.,24, 363 (1972).
Rosenberg, E., Zuckerberg, A., Rubinowitz, C. and Gutnick, D. L.,“Emulsifier ofArthrobacter RAG-1: Isolation and Emulsifying Properties”,Appl Environ. MicrobioL,37, 402 (1979a).
Rosenberg, E., Perry, A., Gibson, D. T. and Gutnick, D. L.,“Emulsifier ofArthrobacter RAG-1: Specificity Hydrocarbon Substrate”,Appl. Environ. MicrobioL,37, 409 (1979b).
Rosenberg, E., Zosim, Z., Belsky, I. and Gutnick, D. L.,“Interaction ofAcinetobacter RAG-1 Emulsan with Hydrocarbon”, Proceeding of the Sixth International Fermentation Symposium, Advances in Biotechnology, Vol. 3, Fermentaton Product, Moo-Young, M., Robinson, C. W. and Vezina, C., eds., Pergamon Press, Toronto, (1980).
Schaefer, E., Synthesis and Release of Emulsan byAcinetobacter calcoaceticus”, Ph.D. Thesis, Massachusetts Institute of Technology, Cambridge, MA (1985).
Sutherland, I. W. and Norval, M.,“The Synthesis of Exopolysaccharide byKlebsiella aerogenes Membrane Preparations and the Involvement of Lipid Intermediates”,Biochem. J.,120, 567 (1970).
Toda, K. and Yabe, I.,“Mathematical Model of Cell Growth and Phosphastase Biosynthesis inSaccharomyces carlsbergensis under Phosphate Limitation”,Biotechnol. Bioeng.,21, 487 (1979).
Troy, F. A., Frerman, F. E. and Heath, E. C.,“The Biosynthesis of Capsular Polysaccharide inAerobacter aerogenes , J. Biol. Chem.,246, 118 (1971).
Wang, S. D. and Wang, D. I. C.,“Cell Adsorption and Local Accumulation of Extracellular Polysaccharide in an ImmobilizedAcinetobacter calcoaceticus System”,Biotechnol. Bioeng.,34, 1261 (1989).
Zuckerberg, A., Diver, A., Perry, Z., Gutnick, D. L. and Rosenberg, E.,“Emulsifier of Arthrobacter RAG-1: Chemical and Physical Properties”,Appl. Environ. MicrobioL,37, 414 (1979).
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Choi, JW., Choi, HG., Lee, SB. et al. Kinetic model for effects of ethanol and phosphate on cell growth and emulsan production inAcinetobacter calcoaceticus RAG-1. Korean J. Chem. Eng. 13, 266–274 (1996). https://doi.org/10.1007/BF02705949
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DOI: https://doi.org/10.1007/BF02705949