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Oxysterols found in opacified cornea of fish

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Lipids

Abstract

We describe a new concept implicating oxidized cholesterol derivatives and very long-chain fatty acids as possible factors in the development of corneal opacification after death. Corneal tissues, removed from both fresh and stale fish eyes, were examined for cholesterol derivatives and fatty acids after methanolysis of lipids. Cholesta-3,5-dien-7-one, cholest-4-en-3-one, hexacosanoic acid, and hexacosenoic acid were identifiedvia gas chromatography/mass spectrometry in opacified corneas, but not in significant amounts in fresh ones. The present study confirmed the presence of lipid hydrolysis and a peroxidation process in the opacified cornea.

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Abbreviations

El:

electron impact

GC/MC:

gas chromatography/mass spectrometry

Rt:

retention time

SIM:

selected-ion-monitoring

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Authors and Affiliations

  1. Department of Legal Medicine, Kobe University School of Medicine, Chuo-ku, 650, Kobe, Japan

    Junko Adachi & Yoshitsugu Tatsuno

  2. Kobe Pharmaceutical University, 658, Kobe, Japan

    Keiko Hojo & Takeaki Naito

Authors
  1. Junko Adachi
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  2. Keiko Hojo
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  3. Takeaki Naito
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  4. Yoshitsugu Tatsuno
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Adachi, J., Hojo, K., Naito, T. et al. Oxysterols found in opacified cornea of fish. Lipids 31, 1039–1043 (1996). https://doi.org/10.1007/BF02522460

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  • DOI: https://doi.org/10.1007/BF02522460

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