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Laterally attached liquid-crystalline polymers as stationary phases. Effect of temperature in RP HPLC and comparison with a commercial C18 stationary phase

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Summary

Specific side-on-fixed liquid-crystalline polymers (SOLCP) have been synthesized for use in silica-modified stationary phases in high-performance liquid chromatography (HPLC). The mesogenic side group of the SOLCP is composed of three benzoate-type phenyl rings with terminal alkoxy chains and is laterally linked to a polysiloxane backbone via an alkyl ester spacer arm. The dependence of the logarithm of the retention factor on the reciprocal temperature showed that the liquid-crystalline anisotropic order was conserved in the small pores (200 Å diameter) of the silica gel. The first-order nematic-isotropic transition is lost and probably becomes second-order. Adsorption enthalpies for the liquid-crystalline stationary phases have been measurement for three polycyclic aromatic hydrocarbon isomers (ortho-terphenyl, triphenylene, and chrysene) and compared with those for a commercial C18 phase. The adsorption enthalpies never exceeded 30 kJ mol−1, i.e. ten times the thermal agitation energy,RT. They were always less on the SOLCP stationary phase than on the C18 column, emphasizing the more rigid structure of the liquid crystalline phase and its mechanism based upon adsorption. Better separation of steroids, pesticides and amino acids were obtained with the LCP-coated silica than the commercial bonded C18 column. Four small peptides were successfully separated by using pure water as mobile phase.

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References

  1. Kelker, H.Z.Anal. Chem. 1963,198, 254.

    Article  CAS  Google Scholar 

  2. Dewar, M.J.S.; Schröeder, J.P.J. Am. Chem. Soc. 1964,86, 5235.

    Article  CAS  Google Scholar 

  3. Kelker, H.; Von Schivizhoffen, E.Adv. Chromatogr. 1968,6, 247.

    CAS  Google Scholar 

  4. Schroeder, J.P. InLiquid Crystals and Plastic Crystals, Vol. 1: Gray, G.W.; Winsor P.A., Eds, Ellis Horwood, Holsted Press, New York,1974, Chap. 7, p. 356.

    Google Scholar 

  5. Janini, G.M.Adv. Chromatogr. 1979,17, 231.

    CAS  Google Scholar 

  6. Witkiewicz, Z.J. Chromatogr. 1982,251, 311.

    Article  CAS  Google Scholar 

  7. Witkiewicz, Z.J. Chromatogr. 1989,466, 37.

    Article  CAS  Google Scholar 

  8. Witkiewicz, Z.; Mazur, J.LC-GC 1990,8, 224.

    CAS  Google Scholar 

  9. Berezkin, V.G.Gazo-Zihidko-Tvierdofaznaya Chromatografiya, Khimia, Moscow,1986.

    Google Scholar 

  10. Mada, H.; Kobayashi, S.Mol. Cryst. Liq. Cryst. 1981,66, 57.

    Google Scholar 

  11. Marciniak, W.; Witkiewicz, Z.J. Chromatogr. 1985,324, 299.

    Article  CAS  Google Scholar 

  12. Marciniak, W.; Witkiewicz, Z.Biul. Wojsk. Akad. Tech. 1986,35(1), 105.

    Google Scholar 

  13. Marciniak, W.; Witkiewicz, Z.Biul. Wojsk. Akad. Tech. 1986,35(4), 37.

    Google Scholar 

  14. Janini, G.M.; Ubeid, M.T.J. Chromatogr. 1982,248, 217.

    Article  CAS  Google Scholar 

  15. Moncton, D.E.; Pindak, R.Phys. Rev. Lett. 1979,43, 701.

    Article  CAS  Google Scholar 

  16. Pershan, P.S.Structure of Liquid Crystal Phases, World Scientific, Singapore,1988.

    Google Scholar 

  17. Bahr, C.; Fliegner, D.Phys. Rev. A 1992,46, 7657.

    Article  CAS  Google Scholar 

  18. Bishop, D.J.; Sprenger, W.O.; Pindak, R.; Neubert, M.E.Phys. Rev. Lett. 1982,49, 1861.

    Article  CAS  Google Scholar 

  19. Rayss, J.; Waksmundski, A.J. Chromatogr. 1984,292, 207.

    Article  CAS  Google Scholar 

  20. Marciniak, W., PhD Thesis, Military Technical Academy, Warsaw,1984.

  21. Nuzzo, R.G.; Allara, D.L.J. Am. Chem. Soc. 1983,105, 4481.

    Article  CAS  Google Scholar 

  22. Ulman, A.Chem. Rev. 1996,96, 1533.

    Article  CAS  Google Scholar 

  23. Wasserman, S.R.; Whitesides, G.M.; Tidswell, I.M.; Ocko, B.M.; Pershan, P.S.; Axe, J.D.J. Am. Chem. Soc. 1989,111, 5852.

    Article  CAS  Google Scholar 

  24. Maoz, R.; Sagiv, J.J. Colloid Interface Sci. 1984,100, 465.

    Article  CAS  Google Scholar 

  25. Badia, A.; Gao, W.; Singh, S.; Demers, L.; Cuccia, L.; Reven, L.Langmuir 1996,12, 1262.

    Article  CAS  Google Scholar 

  26. Pursch, M.; Vanderhart, D.L.; Sander, L.C.; Gu, X.; Nguyen, T.; Wise, S.A.; Gajewski, D.A.,J. Am. Chem. Soc. 2000,122, 6997.

    Article  CAS  Google Scholar 

  27. Albert, K.Trends Anal. Chem. 1998,17, 648.

    Article  CAS  Google Scholar 

  28. Pesek, J.J.; Cash, T.Chromatographia 1989,27, 559.

    Article  CAS  Google Scholar 

  29. Pesek, J.J.; Matyska, M.; Williamsen, E.J.; Tam, R.; Wang, Z.J. Liq. Chromatogr. Related Technol. 1998,21, 2747.

    CAS  Google Scholar 

  30. Delaurent, C.; Tomao, V.; Siouffi, A.M.Chromatographia 1997,47, 355.

    Google Scholar 

  31. Gritti, F.; Félix, G.; Achard, M.-F.; Hardouin, F.J. Chromatogr. A 2000,893, 359.

    Article  CAS  Google Scholar 

  32. Gilpin, R.K.J. Chromatogr. Sci. 1984,22, 371.

    CAS  Google Scholar 

  33. Pesek, J.J.; Siouffi, A.M.Anal. Chem. 1989,61, 1928.

    Article  CAS  Google Scholar 

  34. Ferroukhi, O.; Guermouche, S.; Guermouche, M.H.; Berdagué, P.; Bayle, J.P.; Lafontaine, E.Chromatographia 1998,48, 823.

    Article  CAS  Google Scholar 

  35. Klein, B.H.; Springer, J.J. Liq. Chromatogr. 1991,14, 1519.

    CAS  Google Scholar 

  36. Gritti, F.; Félix, G.; Achard, M.-F., Hardouin, F.Chromatographia 2001,53, 201.

    Article  CAS  Google Scholar 

  37. Terrien, I., Ph.D.Thesis, Université Bordeaux I, No. 1911,1998.

  38. Terrien, I.; Achard, M.-F.; Félix, G.; Hardouin, F.J. Chromatogr. A 1998,810, 19.

    Article  CAS  Google Scholar 

  39. Terrien, I.; Félix, G.; Laguerre, M.; Achard, M.-F.; Hardouin, F.Mol. Cryst. Liq. Cryst. 1999,331, 431.

    Google Scholar 

  40. Gritti, F.; Félix, G.; Achard, M.-F.; Hardouin, F.;J. Chromatogr. A 2000,897, 131.

    Article  CAS  Google Scholar 

  41. Gritti, F.; Félix, G.; Achard, M.-F.; Hardouin, F.J. Chromatogr. A 2001,913, 147.

    Article  CAS  Google Scholar 

  42. Gritti, F.; Félix, G.; Achard, M.-F.; Hardouin, F.J. Chromatogr. A 2001,922, 37.

    Article  CAS  Google Scholar 

  43. Gritti, F.; Félix, G.; Achard, M.-F., Hardouin, F.;J. Chromatogr. A 2001,922, 51.

    Article  CAS  Google Scholar 

  44. Keller, P.; Hardouin, F.; Mauzac, M.Mol. Cryst. Liq. Cryst. 1988,155, 171.

    CAS  Google Scholar 

  45. Mauzac, M.; Hardouin, F.; Richard, H.; Achard, M.-F.; Sigaud, G.; Gasparoux, H.,Eur. Polym. J. 1986,22, 137.

    Article  CAS  Google Scholar 

  46. Gray, G.W.; Hill, J.S.; Lacey, D.Angew. Chem. Int. Engl. Adv. Mater. 1989,28, 1120.

    Article  Google Scholar 

  47. Lecommandoux, S., PhDThesis, Université Bordeaux I, No. 1610,1996.

  48. Rekker, R.F. InThe Hydrophobic Fragmental Constant, Elsevier, Amsterdam,1977.

    Google Scholar 

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Authors and Affiliations

  1. E.N.S.C.P.B., Université Bordeaux I, Avenue Pey-Berland, 33607, Pessac, France

    F. Girtti & G. Félix

  2. C.R.P.P.-CNRS, Université Bordeaux I, Avenue du Dr Schweitzer, 33600, Pessac, France

    F. Girtti, M. -F. Achard & F. Hardouin

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  1. F. Girtti
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  2. G. Félix
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  3. M. -F. Achard
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  4. F. Hardouin
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Girtti, F., Félix, G., Achard, M.F. et al. Laterally attached liquid-crystalline polymers as stationary phases. Effect of temperature in RP HPLC and comparison with a commercial C18 stationary phase. Chromatographia 56, 9–17 (2002). https://doi.org/10.1007/BF02490240

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  • DOI: https://doi.org/10.1007/BF02490240

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