Abstract
The use of the low-angle x-ray diffraction method in biology is described. Some x-ray diffraction patterns have been known since the early 1930's but, in the last decade, many new patterns, which show more reflections than previously, have been recorded. An interpretation of these improved patterns of biological systems provides detailed information on the molecular structure of living cells. The theory and the current status of the low-angle x-ray diffraction method is presented. A brief account of the x-ray studies on collagen, muscle, nerve and membranes is given. Emphasis is given to the history of ideas underlying progress in this area and the current unsolved problems in this field of biophysical research are indicated and discussed.
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References
H. E. Huxley,Ph.D. thesis., (1952) University of Cambridge, England.
C. R. Worthington,Ph.D. thesis., (1955) Adelaide University, Australia.
W. Ehrenberg and W. E. Spear,Proc. Phys. Soc.,64 (1951) 67.
G. F. Elliott and C. R. Worthington,J. Ultrastruct. Res.,91 (1963) 166.
H. E. Huxley and W. Brown,J. Mol. Biol.,30 (1967) 383.
C. R. Worthington,Probes of Structure and Function of Macromolecules and Membranes, Eds. B. Chance, C. P. Lee and J. K. Blasie, Vol. 1 (Academic Press, New York, 1971) 179.
G. Rosenbaum, K. C. Holmes and J. Witz,Nature 230 (1971) 434.
Y. Dupont, A. Gabriel, M. Chambre, T. Gulik-Krzywicki and E. Shackter,Nature 238 (1972) 331.
C. J. Borkowski and M. K. Kopp,Rev. Sci. Inst.,39 (1968) 1515.
M. Chambre and A. Caraggioni,Nature 244 (1973) 118.
A. E. Blaurock and C. R. Worthington,Biophys. J. (1966).
C. R. Worthington,Exp. Eye Res., (1973) in press.
C. R. Worthington, G. I. King and T. J. McIntosh,Biophys. J.,13 (1973) 480.
C. R. Worthington,Curr. Top. Bioenerg.,5 (1973) 1.
W. T. Astbury,Trans. Far. Soc.,34 (1938) 378.
W. T. Astbury,J. Intern. Soc. Leather Trades' Chim.,24 (1940) 69.
L. Pauling and R. B. Corey,Proc. Nat. Acad. Sci.,37 (1951) 272.
G. N. Ramachandran and G. Kartha,Nature 176 (1955) 593.
A. Rich and F. H. C. Crick,Nature 176 (1955) 915.
P. M. Cowan, S. McGavin and A. T. C. North,Nature 176 (1955) 1062.
R. E. Burge,Structure and Function of Connective and Skeletal Tissue, Eds. S. F. Jackson, R. D. Harkness, S. M. Partridge and G. R. Tristram (Butterworth's London, 1965).
R. S. Bear,J. Am. Chem. Soc.,64 (1942) 727.
F. O. Schmitt, C. E. Hall and M. A. Jakus,J. Am. Chem. Soc.,64 (1942) 1234.
S. G. Tomlin and C. R. Worthington,Proc. Roy. Soc. A.,235 (1956) 189.
D. O. Ellis and S. McGavin,J. Ultrastr. Res.,32 (1970) 191.
R. S. Bear,J. Am. Chem. Soc.,66 (1944) 1297.
P. Kaesberg and M. M. Shurman,Biochim. Biophys. Acta,2 (1953) 1.
C. R. Worthington and S. G. Tomlin,Nature 175 (1955) 811.
J. Hanson,Quart. Rev. of Biophysics,1 (1968) 177.
G. F. Elliott, J. Lowy and B. M. Millman,Nature 206 (1965) 1357.
H. E. Huxley, W. Brown and K. C. Holmes,Nature 206 (1965) 1358.
C. R. Worthington,J. Mol. Biol.,3 (1961) 618.
H. E. Huxley and J. Hanson,Nature 173 (1954) 973.
A. F. Huxley and R. Niedergerke,Nature 173 (1954) 971.
H. E. Huxley,Proc. Roy. Soc. B.,141 (1953) 59.
H. E. Huxley,Biochem. Biophys. Acta,12 (1953) 387.
H. E. Huxley,J. Biophys. Biochem. Cytol.,3 (1957) 631.
W. T. Astbury and L. C. Spark,Biochim. Biophys. Acta,1 (1947) 388.
R. S. Bear,J. Amer. Chem. Soc.,67 (1945) 1625.
C. C. Selby and R. S. Bear,J. Biophys. Biochem. Cytol.,2 (1956) 71.
C. R. Worthington,J. Mol. Biol.,1 (1959) 398.
J. Hanson and J. Lowy,J. Mol. Biol.,6 (1963) 46.
H. E. Huxley and W. Brown,J. Mol. Biol.,30 (1967) 383.
B. M. Millman, G. F. Elliott and J. Lowy,Nature 213 (1967) 356.
G. F. Elliott,Proc. Roy. Soc. B.,160 (1964) 467.
A. Miller and R. T. Tregear,J. Mol. Biol.,70 (1972) 85.
G. F. Elliott, J. Lowy and C. R. Worthington,J. Mol. Biol.,6 (1963) 295.
G. F. Elliott,J. Theoret. Biol.,21 (1968) 71.
E. Rome,J. Mol. Biol.,37 (1968) 331.
G. F. Elliott, J. Lowy and C. R. Worthington,J. Mol. Biol.,25 (1967) 31.
H. E. Huxley,Proc. Roy. Soc. B.,178 (1971) 131.
M. Spencer and C. R. Worthington,Nature 187 (1960) 388.
W. J. Schmidt,Z. Zellforsch. Mikroskop. Anat.,23 (1936) 657.
G. Boehm,Kolloid Z.,62 (1933) 22.
F. O. Schmitt, R. S. Bear and G. L. Clark,Radiology,25 (1935) 131.
A. E. Blaurock and C. R. Worthington,Biochim. Biophys. Acta,173 (1969) 419.
F. O. Schmitt, R. S. Bear and K. J. Palmer,J. Cell Comp. Physiol.,18 (1941) 31.
C. R. Worthington and A. E. Blaurock,Nature 218 (1968) 87.
J. B. Finean and P. F. Millington,J. Biophys. Biochem. Cytol.,3 (1957) 89.
J. B. Finean and R. E. Burge,J. Mol. Biol.,7 (1963) 672.
M. R. Moody,Science,142 (1963) 1173.
C. R. Worthington and A. E. Blaurock,Biochim. Biophys. Acta,173 (1969) 427.
T. J. McIntosh and C. R. Worthington,Biophys. J., (1974) in press.
C. R. Worthington,Proc. Nat. Acad. Sci.,63 (1969) 604.
C. R. Worthington,Ann. N. Y. Acad. Sci.,195 (1972) 293.
C. R. Worthington and T. J. McIntosh,Nature 245 (1973) 97.
J. B. Finean,Quart. Rev. Biophys.,2 (1969) 1.
C. R. Worthington,Biophysics and Physiology of Excitable Membranes, Ed. W. J. Adelman (Van Nostrand-Reinhold, New Jersey, 1971).
Y. K. Levine,Progr. in Biophys. and Mol. Biol.,24 (1972) 1.
G. G. Shipley,Biological Membranes, Ed. D. Chapman, Vol. II (Academic Press, New York, 1973).
J. B. Finean, R. Coleman, W. G. Green and A. R. Limbrick,J. Cell Sci.,1 (1966) 287.
J. B. Finean, R. Coleman, S. Knutton, A. R. Limbrick and J. E. Thompson,J. Gen. Physiol.,51 (1968) 190.
M. H. F. Wilkins, A. E. Blaurock and D. M. Engelman,Nature 230 (1971) 72.
J. K. Blasie, M. M. Dewey, A. E. Blaurock and C. R. Worthington,J. Mol. Biol.,14 (1965) 143.
J. K. Blasie, C. R. Worthington and M. M. Dewey,J. Mol. Biol.,39 (1969) 407.
J. K. Blasie and C. R. Worthington,J. Mol. Biol.,39 (1969) 417.
W. J. Gras and C. R. Worthington,Proc. Nat. Acad. Sci.,63 (1969) 233.
A. E. Blaurock and M. H. F. Wilkins,Nature 223 (1969) 906.
J. M. Corless,Nature 237 (1972) 229.
C. R. Worthington,J. Mol. Biol.,2 (1960) 327.
J. E. Thompson, R. Coleman and J. B. Finean,Biochim. Biophys. Acta,150 (1968) 405.
A. R. Limbrick and J. B. Finean,J. Cell Sci.,7 (1970) 373.
D. A. Goodenough and W. Stoeckenius,J. Cell Biol.,54 (1972) 646.
D. M. Engelman,J. Mol. Biol.,58 (1971) 153.
A. E. Blaurock and W. Stoeckenius,Nature 233 (1971) 152.
S. C. Harrison, D. L. D. Caspar, R. D. Camerini-Otero and R. M. Franklin,Nature 229 (1971) 197.
C. R. Worthington and S. S. Liu,Arch. Biochem. Biophys.,157 (1973) 573.
Y. Dupont, S. C. Harrison and W. Hasselback,Nature 244 (1973) 555.
E. Gorter and R. Grendel,J. Exptl. Med.,41 (1925) 439.
J. F. Danielli and H. A. Davson,J. Cell. Comp. Physiol.,5 (1935) 495.
J. D. Robertson,Biochem. Soc. Symposia,16 (1959) 3.
C. R. Worthington,Fed. Proc.,30 (1971) 57.
L. Warren and M. C. Glick,Biomembranes, Ed. L. A. Manson (Plenum Press, London, 1971).
J. D. Watson and F. H. C. Crick,Nature 171 (1953) 737.
M. H. F. Wilkins, A. R. Stokes and H. R. Wilson,Nature 171 (1953) 737.
S. Arnott,Progr. in Biophys. and Mol. Biol.,22 (1971) 181.
W. T. Astbury,Proc. Roy. Soc. B.,134 (1947) 303.
N. R. Silvester,J. Mol. Biol.,8 (1964) 11.
J. N. Champness,J. Mol. Biol.,56 (1971) 295.
C. Cohen,Principles of Biomolecules Organization, Ed. G. E. W. Wolstenholme and M. O'Connor (Churchill, London, 1965).
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Worthington, C.R. Low angle x-ray diffraction and biology. J Biol Phys 2, 1–25 (1974). https://doi.org/10.1007/BF02309370
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DOI: https://doi.org/10.1007/BF02309370