Summary
A new amperometric detector with two sensor electrodes for high-performance liquid chromatography has been designed and tested. Its construction was based on the design of our earlier polarographic flow-through detector [1] and contains two flow-through micro cells, connected in series, machined in the detector body. Upstream is a thin-layer cell with a glassy carbon or carbon paste electrode and downstream the polarographic micro cell with the rapidly drop** mercury electrode (RDME). This arrangement of the first, integrating and the second, nonintegrating sensors is the reason why extra-column effects caused by the detection are small. These extra-column effects were estimated by analysing retention data for a mixture of o-, m-, p-nitrophenols and o-, m-, p-nitroanilines, that is model compounds with similar diffusion coefficients, electroactive at both sensor electrodes. This new detector when combined with a bipotentiostat and a dual-pen recorder may be used for simultaneous detection of compounds in the eluate that can either be reduced at negative potentials at the RDME or oxidized at positive potentials at the carbon electrode. The detector was applied for simultaneous detection of nitrofuran derivatives used as bacteriostatic drugs and their potential metabolites, in reversed-phase HPLC.
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Presented at the 14th International Symposium on Chromatography, London, September, 1982.
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Kutner, W., Kemula, W. High-performance LC Bi-electrode amperometric flow-through detector with a carbon-drop** mercury sensor-electrode system. Chromatographia 17, 322–327 (1983). https://doi.org/10.1007/BF02270667
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DOI: https://doi.org/10.1007/BF02270667