Summary
Primary cultures of rat myocardial cells which were subjected to oxygen and glucose deprivation, 2 conditions associated with ischemia, were evaluated for alterations in lysosomal integrity. A photometric technique measured changes in latent acid phosphatase activity and lysosomal membrane permeability.
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References
M. A. Ricciutti, Am. J. Cardiol.30, 492 (1972).
S. Hoffstein, G. Weissmann and A. C. Fox, Circulation53, 1 (1976).
R. S. Decker, A. R. Poole, E. E. Griffin, J. T. Dingle and K. Wildenthal, J. clin. Invest.59, 911 (1977).
D. Acosta and M. Puckett, In Vitro13, 168 (1977).
D. Acosta and M. Puckett, In Vitro13, 818 (1978).
A. A. Aikman and E. D. Wills, Radiat. Res.57, 403 (1974).
D. G. Wenzel, J. W. Wheatley and G. D. Byrd, Toxic. appl. Pharmac.11, 215 (1970).
D. Acosta and D. G. Wenzel, Atherosclerosis20, 417 (1974).
B. L. Reed and D. G. Wenzel, Histochem. J.7, 115 (1975).
L. Bitensky, R. G. Butcher and J. Chayen, in: Lysosomes in Biology and Pathology, vol. 3, p. 465. Ed. J. T. Dingle. North-Holland Publishing Co., Amsterdam 1973.
L. D. Hillis and E. Braunwald, New Engl. J. Med.296, 971 (1977).
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Acknowledgments: The authors thank Dora Fernandez for skillful technical assistance. Research was supported by a grant from the National Heart, Lung, and Blood Institute, HL 18647.
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Acosta, D., Puckett, M. & McMillin, R. Ischemic myocardial injury in cultured heart cells: In situ lysosomal damage. Experientia 34, 1388–1389 (1978). https://doi.org/10.1007/BF01981493
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DOI: https://doi.org/10.1007/BF01981493