Abstract
Indirect immunofluorescence assays to detect antibodies toCandida albicans blastospore and germ tube were performed in sera of 29 bone marrow transplant patients. Antibodies to germ tube were present in the sera of six patients, in four of whom aCandida albicans infection was highly probable, while in the other two patients it was not possible to determine the previous course. No healthy blood donors had these antibodies. On the other hand, detection of antibodies toCandida albicans blastospore showed low specificity in the diagnosis of systemic candidiasis. These preliminary findings suggest that the detection of antibodies toCandida albicans germ tube may be an important aid in the diagnosis of systemic candidiasis in bone marrow transplant patients.
Similar content being viewed by others
References
Verfaillie C, Weisdorf D, Haake R, Hostetter M, Ramsay NKC, McGlave P Candida infection in bone marrow transplant recipients. Bone Marrow Transplantation 1991, 8: 177–184.
Goodrich JM, Reed EC, Mori M, Fisher LD, Skerret S, Dandliker PS, Klis B, Counts GW, Meyers JD Clinical features and analysis of risk factors for invasive candidal infection after marrow transplantation. Journal of Infectious Diseases 1991, 164: 731–740.
de Repentigny L Serological techniques for diagnosis of fungal infection. European Journal of Clinical Microbiology & Infectious Diseases 1989, 8: 362–373.
Hopwood V, Warnock DW New developments in the diagnosis of opportunistic fungal infection. European Journal of Clinical Microbiology 1986, 5: 379–388.
Sundstrom PM, Kenny GE Characterization of antigens specific to the surface of germ tubes ofCandida albicans by immunofluorescence. Infection and Immunity 1984, 43: 850–855.
Pontón J, Jones JM Analysis of cell wall extracts ofCandida albicans by sodium dodecyl sulfate-polycrylamide gel electrophoresis and Western blot techniques. Infection and Immunity 1986, 53: 565–572.
Quindós G, Pontón J, Cisterna R Detection of antibodies toCandida albicans germ tube in the diagnosis of systemic candidiasis. European Journal of Clinical Microbiology 1987, 6: 142–146.
Esterly NB Serum antibody titers toCandida albicans utilizing an immunofluorescent technique. American Journal of Clinical Pathology 1979, 32: 399–405.
Ho YM, Ng MH, Huang CT Antibodies to germinating and yeast cells ofCandida albicans in human and rabbit sera. Journal of Clinical Pathology 1979, 32: 399–405.
Evans EGV, Richardson MD, Odds FC, Holland KT Relevance of antigenicity ofCandida albicans growth phases to diagnosis of systemic candidosis. British Medical Journal 1973, 4: 86–87.
Pontón J, Jones JM Identification of two germ-tubespecific cell wall antigens ofCandida albicans. Infection and Immunity 1986, 54: 864–868.
Quindós G, Pontón J, Cisterna R, Mackenzie DWR Value of detection of antibodies toCandida albicans germ tube in the diagnosis of systemic candidiasis. European Journal of Clinical Microbiology & Infectious Diseases 1990, 9: 178–183.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Villalba, R., González, A.I., Linares, M.J. et al. Detection of antibodies toCandida albicans germ tube as a possible aid in diagnosing systemic candidiasis in bone marrow transplant patients. Eur. J. Clin. Microbiol. Infect. Dis. 12, 347–349 (1993). https://doi.org/10.1007/BF01964431
Issue Date:
DOI: https://doi.org/10.1007/BF01964431