Summary
Variations in membrane potential difference (PD) were observed between root cortical cells ofAllium porrum plants, uninfected and infected withGlomus E3: cell membrane potential was constantly hyperpolarised in the latter during the time course of infection and at all degrees of infection. This hyperpolarisation persisted in the presence of an inhibitor (2,4-dinitrophenol) and of an activator (fusicoccin) of transport membrane processes, suggesting that long-distance signals generated by the fungus could be involved.
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Abbreviations
- PD:
-
membrane potential difference
- VAM:
-
vesicular arbuscular mycorrhizae
- DNP:
-
2,4-dinitrophenol
- FC:
-
fusicoccin
- EB:
-
erythrosin
References
Berta G, Fusconi A, Trotta A, Scannerini S (1990) Morphogenetic modifications induced by the mycorrhizal fungusGlomus strain E3 in the root system ofAllium porrum L. New Phytol 114: 207–215
—, Tagliasacchi AM, Fusconi A, Gerlero D, Trotta A, Scannerini S (1991) The mitotic cycle in root apical meristems ofAllium porrum L. is controlled by the endomycorrhizal fungusGlomus sp. strain E3. Protoplasma 161: 12–16
Bonfante-Fasolo P, Perotto S (1992) Plants and endomycorrhizal fungi: the cellular and molecular basis of their interaction. In: Verma DPS (ed) Molecular signals in plant-microbe communications. CRC Press, Boca Raton, pp 445–470
Cocucci MC, Marré E (1986) Erythrosin B as an effective inhibitor of electrogenic H+ extrusion. Plant Cell Environ 9: 676–679
Fusconi A, Berta G, Scannerini S, Trotta A (1986) Meristematic activity in mycorrhizal and uninfected roots ofAllium porrum L. In: Gianinazzi-Pearson V, Gianinazzi S (eds) Physiological and genetical aspects of mycorrhizae. INRA Press, Paris, pp 667–672
Gianinazzi-Pearson V, Gianinazzi S (1988) Morphological integration and functional compatibility between symbionts in vesicular arbuscolar endomycorrhizal associations. In: Scannerini S, Smith D, Bonfante-Fasolo P, Gianinazzi-Pearson V (eds) Cell to cell signals in plant, animal and microbial symbiosis. Springer, Berlin Heidelberg New York Tokyo, pp 73–84
—, Smith SE, Gianinazzi S, Smith FA (1991) Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhizas V. Is the H+-ATPase a component of ATP-hydrolysing enzyme activities in plant-fungus interfaces? New Phytol 117: 61–74
Hepper CM (1977) A colorimetric method for estimating vesiculararbuscular mycorrhizal infection in roots. Soil Biol Biochem 9: 15–18
Lehninger AL (1977) Biochemistry. Worth, New York
Marrè E (1979) Fusicoccin: a tool in plant physiology. Annu Rev Plant Physiol 30: 273–288
—, Ballarin-Denti A (1985) Proton pumps of plasmalemma and tonoplast. J Bioenerg Biomemb 17: 1–21
—, Lado P, Rasi-Caldogno F, Colombo R, Cocucci M, De Michelis MT (1975) Regulation of proton extrusion by plant hormones and cell elongation. Physiol Veg 13: 797–811
Pellissier B, Thibaud JB, Grignon C, Esqerré-Tugayé MT (1986) Cell surfaces in plant-microorganism interactions. VII. Elicitor preparations from two fungal pathogens depolarize plant membranes. Plant Sci 46: 103–109
Romani G, Marré MT, Bellando M, Alloatti G, Marré E (1985) H+ extrusion and K+ uptake associated with potential hyperpolarisation in maize and wheat root segments treated with permanent weak acids. Plant Physiol 79: 734–739
Scannerini S (1991) Recognition and cell signals in mutualistic symbioses. In: Neuhoff V, Friend J (eds) Cell to cell signals in plants and animals. Springer, Berlin Heidelberg New York Tokyo, pp 39–60 (NATO ASI Series, series H, vol 51)
Serrano R (1985) Plasma membrane ATPase of plants and fungi. CRC Press, Boca Raton
Sijmons PC, Lanfermeijer FC, De Boer AH, Prins HBA, Bienfait HF (1984) Depolarization of cell membrane potential during trans-plasma membrane electron transfer to electron acceptors in iron-deficit roots ofPhaseolus vulgaris L. Plant Physiol 76: 943–946
Smith SE, Gianinazzi-Pearson V (1988) Physiological interactions between symbionts in vesicular-arbuscolar mycorrhizal plants. Annu Rev Plant Physiol Plant Mol Biol 39: 221–244
—, Smith FA (1990) Structure and function of the interfaces in biotrophic symbioses as they relate to nutrient transport. New Phytol 114: 1–38
Spanu P, Bonfante-Fasolo P (1988) Cell-wall-bound peroxidase activity in roots of mycorrhizalAllium porrum. New Phytol 109: 119–124
—, Boiler T, Ludwig A, Wiemken A, Faccio A, Bonfante-Fasolo P (1989) Chitinase in roots of mycorrhizalAllium porrum: regulation and localization. Planta 117: 447–455
Ullrich CL, Novacky AJ (1991) Electrical membrane properties of leaves roots and single root cap cells of susceptibleAvena saliva. Plant Physiol 95: 675–681
Wieser U, Pankow W, Wiemken A (1986) The adenylate energy charge of vesicular-arbuscularmycorrhiza of onions (Allium cepa L.). J Plant Physiol 124: 181–186
Zocchi G, Cocucci S (1989) Fe uptake mechanism in Fe-efficient cucumber roots. Plant Physiol 92: 908–916
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Fieschi, M., Alloatti, G., Sacco, S. et al. Membrane potential hyperpolarisation in vesicular arbuscular mycorrhizae ofAllium porrum L.: a non-nutritional long-distance effect of the fungus. Protoplasma 168, 136–140 (1992). https://doi.org/10.1007/BF01666259
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DOI: https://doi.org/10.1007/BF01666259